5w7f - Revision history

OCA at 08:29, 8 January 2020

2020-01-08T08:29:21Z

←Older revision		Revision as of 08:29, 8 January 2020
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	==Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with lipid A==		==Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with lipid A==
-	<StructureSection load='5w7f' size='340' side='right' caption='[[5w7f]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5w7f' size='340' side='right'caption='[[5w7f]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5w7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[5w7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7F FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Acyloxyacyl hydrolase]]		[[Category: Acyloxyacyl hydrolase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Gorelik, A]]		[[Category: Gorelik, A]]

OCA at 07:08, 31 January 2018

2018-01-31T07:08:18Z

←Older revision		Revision as of 07:08, 31 January 2018
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/AOAH_MOUSE AOAH_MOUSE]] Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS) (PubMed:12810692, PubMed:15155618, PubMed:17322564, PubMed:19860560). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (PubMed:17322564, PubMed:19860560, PubMed:28622363). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (PubMed:18779055).<ref>PMID:12810692</ref> <ref>PMID:15155618</ref> <ref>PMID:17322564</ref> <ref>PMID:18779055</ref> <ref>PMID:19860560</ref> <ref>PMID:28622363</ref>		[[http://www.uniprot.org/uniprot/AOAH_MOUSE AOAH_MOUSE]] Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS) (PubMed:12810692, PubMed:15155618, PubMed:17322564, PubMed:19860560). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (PubMed:17322564, PubMed:19860560, PubMed:28622363). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (PubMed:18779055).<ref>PMID:12810692</ref> <ref>PMID:15155618</ref> <ref>PMID:17322564</ref> <ref>PMID:18779055</ref> <ref>PMID:19860560</ref> <ref>PMID:28622363</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	LPS is a potent bacterial endotoxin that triggers the innate immune system. Proper recognition of LPS by pattern-recognition receptors requires a full complement of typically six acyl chains in the lipid portion. Acyloxyacyl hydrolase (AOAH) is a host enzyme that removes secondary (acyloxyacyl-linked) fatty acids from LPS, rendering it immunologically inert. This activity is critical for recovery from immune tolerance that follows Gram-negative infection. To understand the molecular mechanism of AOAH function, we determined its crystal structure and its complex with LPS. The substrate's lipid moiety is accommodated in a large hydrophobic pocket formed by the saposin and catalytic domains with a secondary acyl chain inserted into a narrow lateral hydrophobic tunnel at the active site. The enzyme establishes dispensable contacts with the phosphate groups of LPS but does not interact with its oligosaccharide portion. Proteolytic processing allows movement of an amphipathic helix possibly involved in substrate access at membranes.
		+
		+	Crystal structure of the mammalian lipopolysaccharide detoxifier.,Gorelik A, Illes K, Nagar B Proc Natl Acad Sci U S A. 2018 Jan 17. pii: 1719834115. doi:, 10.1073/pnas.1719834115. PMID:29343645<ref>PMID:29343645</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5w7f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:16, 17 January 2018

2018-01-17T08:16:55Z

←Older revision		Revision as of 08:16, 17 January 2018
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	<StructureSection load='5w7f' size='340' side='right' caption='[[5w7f]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5w7f' size='340' side='right' caption='[[5w7f]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w7f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w7f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=FTT:3-HYDROXY-TETRADECANOIC+ACID'>FTT</scene>, <scene name='pdbligand=GCS:D-GLUCOSAMINE'>GCS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=FTT:3-HYDROXY-TETRADECANOIC+ACID'>FTT</scene>, <scene name='pdbligand=GCS:D-GLUCOSAMINE'>GCS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aoah ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acyloxyacyl_hydrolase Acyloxyacyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.77 3.1.1.77] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acyloxyacyl_hydrolase Acyloxyacyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.77 3.1.1.77] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w7f OCA], [http://pdbe.org/5w7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w7f RCSB], [http://www.ebi.ac.uk/pdbsum/5w7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w7f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w7f OCA], [http://pdbe.org/5w7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w7f RCSB], [http://www.ebi.ac.uk/pdbsum/5w7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w7f ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Acyloxyacyl hydrolase]]		[[Category: Acyloxyacyl hydrolase]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Gorelik, A]]		[[Category: Gorelik, A]]
	[[Category: Illes, K]]		[[Category: Illes, K]]

OCA at 05:47, 3 January 2018

2018-01-03T05:47:39Z

←Older revision		Revision as of 05:47, 3 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5w7f is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with lipid A==
-		+	<StructureSection load='5w7f' size='340' side='right' caption='[[5w7f]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5w7f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W7F FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=FTT:3-HYDROXY-TETRADECANOIC+ACID'>FTT</scene>, <scene name='pdbligand=GCS:D-GLUCOSAMINE'>GCS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acyloxyacyl_hydrolase Acyloxyacyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.77 3.1.1.77] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w7f OCA], [http://pdbe.org/5w7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w7f RCSB], [http://www.ebi.ac.uk/pdbsum/5w7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w7f ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AOAH_MOUSE AOAH_MOUSE]] Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS) (PubMed:12810692, PubMed:15155618, PubMed:17322564, PubMed:19860560). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (PubMed:17322564, PubMed:19860560, PubMed:28622363). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (PubMed:18779055).<ref>PMID:12810692</ref> <ref>PMID:15155618</ref> <ref>PMID:17322564</ref> <ref>PMID:18779055</ref> <ref>PMID:19860560</ref> <ref>PMID:28622363</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acyloxyacyl hydrolase]]
		+	[[Category: Gorelik, A]]
		+	[[Category: Illes, K]]
		+	[[Category: Nagar, B]]
		+	[[Category: Gdsl esterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lipopolysaccharide]]
		+	[[Category: Lp]]
		+	[[Category: Saposin]]

OCA: Protected "5w7f" [edit=sysop:move=sysop]

2017-09-27T14:32:08Z

Protected "5w7f" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:32, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5w7f is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2017-09-27T14:32:06Z

New page: '''Unreleased structure''' The entry 5w7f is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5w7f is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]