5w9p - Revision history

OCA at 22:20, 10 April 2020

2020-04-10T22:20:48Z

OCA at 19:56, 6 March 2020

2020-03-06T19:56:55Z

OCA at 09:48, 1 January 2020

2020-01-01T09:48:24Z

OCA at 10:27, 8 November 2017

2017-11-08T10:27:56Z

←Older revision

Revision as of 10:27, 8 November 2017

OCA: Protected "5w9p" [edit=sysop:move=sysop]

2017-08-30T04:07:50Z

Protected "5w9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 30 August 2017

OCA: New page: ==MERS S ectodomain trimer in complex with variable domain of neutralizing antibody G4== == Structural highlights ==
<table><tr><td colspan='2'>[[5w9p]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W9P FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w9p OCA], [http://pdbe.org/5w9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w9p RCSB], [http://www.ebi.ac.uk/pdbsum/5w9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w9p ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Middle East respiratory syndrome coronavirus (MERS-CoV) is a lineage C betacoronavirus that since its emergence in 2012 has caused outbreaks in human populations with case-fatality rates of approximately 36%. As in other coronaviruses, the spike (S) glycoprotein of MERS-CoV mediates receptor recognition and membrane fusion and is the primary target of the humoral immune response during infection. Here we use structure-based design to develop a generalizable strategy for retaining coronavirus S proteins in the antigenically optimal prefusion conformation and demonstrate that our engineered immunogen is able to elicit high neutralizing antibody titers against MERS-CoV. We also determined high-resolution structures of the trimeric MERS-CoV S ectodomain in complex with G4, a stem-directed neutralizing antibody. The structures reveal that G4 recognizes a glycosylated loop that is variable among coronaviruses and they define four conformational states of the trimer wherein each receptor-binding domain is either tightly packed at the membrane-distal apex or rotated into a receptor-accessible conformation. Our studies suggest a potential mechanism for fusion initiation through sequential receptor-binding events and provide a foundation for the structure-based design of coronavirus vaccines.

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen.,Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS Proc Natl Acad Sci U S A. 2017 Aug 14. pii: 201707304. doi:, 10.1073/pnas.1707304114. PMID:28807998<ref>PMID:28807998</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5w9p" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Pallesen, J]]
[[Category: Ward, A B]]
[[Category: Antibody]]
[[Category: G4]]
[[Category: Immunogen]]
[[Category: Mer]]
[[Category: Mers s protein]]
[[Category: Peplomer]]
[[Category: Viral protein]]
[[Category: Viral spike]]