5wj9 - Revision history

OCA at 22:24, 10 April 2020

2020-04-10T22:24:10Z

←Older revision		Revision as of 22:24, 10 April 2020
Line 3:		Line 3:
	<SX load='5wj9' size='340' side='right' viewer='molstar' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>		<SX load='5wj9' size='340' side='right' viewer='molstar' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AQV:2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione'>AQV</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AQV:2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione'>AQV</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCOLN1, ML4, MSTP080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCOLN1, ML4, MSTP080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj9 OCA], [http://pdbe.org/5wj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj9 OCA], [http://pdbe.org/5wj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj9 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 19:58, 6 March 2020

2020-03-06T19:58:27Z

←Older revision		Revision as of 19:58, 6 March 2020
Line 1:		Line 1:

	==Human TRPML1 channel structure in agonist-bound open conformation==		==Human TRPML1 channel structure in agonist-bound open conformation==
-	<~~StructureSection~~ load='5wj9' size='340' side='right'caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>	+	<SX load='5wj9' size='340' side='right' viewer='molstar' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>
Line 24:		Line 24:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 09:11, 18 December 2019

2019-12-18T09:11:18Z

←Older revision		Revision as of 09:11, 18 December 2019
Line 1:		Line 1:

	==Human TRPML1 channel structure in agonist-bound open conformation==		==Human TRPML1 channel structure in agonist-bound open conformation==
-	<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>	+	<StructureSection load='5wj9' size='340' side='right'caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Li, X]]		[[Category: Li, X]]
	[[Category: Schmiege, P]]		[[Category: Schmiege, P]]
	[[Category: Human trpml1]]		[[Category: Human trpml1]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 09:28, 1 November 2017

2017-11-01T09:28:47Z

←Older revision		Revision as of 09:28, 1 November 2017
Line 1:		Line 1:

-	==Human TRPML1 channel ~~structures~~ in agonist-bound open ~~conformations~~==	+	==Human TRPML1 channel structure in agonist-bound open conformation==
	<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>		<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AQV:2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione'>AQV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AQV:2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione'>AQV</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCOLN1, ML4, MSTP080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj9 OCA], [http://pdbe.org/5wj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj9 OCA], [http://pdbe.org/5wj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj9 ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Li, X]]		[[Category: Li, X]]
	[[Category: Schmiege, P]]		[[Category: Schmiege, P]]
	[[Category: Human trpml1]]		[[Category: Human trpml1]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 06:56, 25 October 2017

2017-10-25T06:56:30Z

←Older revision		Revision as of 06:56, 25 October 2017
Line 1:		Line 1:

-	==~~cryo~~-~~EM of a lysosomal protein~~==	+	==Human TRPML1 channel structures in agonist-bound open conformations==
	<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>		<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MCLN1_HUMAN MCLN1_HUMAN]] Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.<ref>PMID:12459486</ref> <ref>PMID:14749347</ref>		[[http://www.uniprot.org/uniprot/MCLN1_HUMAN MCLN1_HUMAN]] Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.<ref>PMID:12459486</ref> <ref>PMID:14749347</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transient receptor potential mucolipin 1 (TRPML1) is a Ca2+-releasing cation channel that mediates the calcium signalling and homeostasis of lysosomes. Mutations in TRPML1 lead to mucolipidosis type IV, a severe lysosomal storage disorder. Here we report two electron cryo-microscopy structures of full-length human TRPML1: a 3.72-A apo structure at pH 7.0 in the closed state, and a 3.49-A agonist-bound structure at pH 6.0 in an open state. Several aromatic and hydrophobic residues in pore helix 1, helices S5 and S6, and helix S6 of a neighbouring subunit, form a hydrophobic cavity to house the agonist, suggesting a distinct agonist-binding site from that found in TRPV1, a TRP channel from a different subfamily. The opening of TRPML1 is associated with distinct dilations of its lower gate together with a slight structural movement of pore helix 1. Our work reveals the regulatory mechanism of TRPML channels, facilitates better understanding of TRP channel activation, and provides insights into the molecular basis of mucolipidosis type IV pathogenesis.
		+
		+	Human TRPML1 channel structures in open and closed conformations.,Schmiege P, Fine M, Blobel G, Li X Nature. 2017 Oct 19;550(7676):366-370. doi: 10.1038/nature24036. Epub 2017 Oct, 11. PMID:29019983<ref>PMID:29019983</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wj9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:19, 18 October 2017

2017-10-18T07:19:21Z

←Older revision		Revision as of 07:19, 18 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wj9 is ~~ON HOLD until Paper Publication~~	+	==cryo-EM of a lysosomal protein==
-		+	<StructureSection load='5wj9' size='340' side='right' caption='[[5wj9]], [[Resolution\|resolution]] 3.49Å' scene=''>
-	~~Authors~~: ~~Schmiege~~, P., Li, X.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5wj9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJ9 FirstGlance]. <br>
-	~~Description~~: ~~cryo-EM~~ of a lysosomal ~~protein~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AQV:2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione'>AQV</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wj9 OCA], [http://pdbe.org/5wj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wj9 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/MCLN1_HUMAN MCLN1_HUMAN]] Mucolipidosis type 4. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCLN1_HUMAN MCLN1_HUMAN]] Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca(2+) transport regulating lysosomal exocytosis.<ref>PMID:12459486</ref> <ref>PMID:14749347</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Li, X]]
	[[Category: Schmiege, P]]		[[Category: Schmiege, P]]
-	[[Category: ~~Li, X~~]]	+	[[Category: Human trpml1]]
		+	[[Category: Membrane protein]]

OCA: Protected "5wj9" [edit=sysop:move=sysop]

2017-09-27T14:53:21Z

Protected "5wj9" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:53, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wj9 is ON HOLD until Paper Publication Authors: Schmiege, P., Li, X. Description: cryo-EM of a lysosomal protein [[Category: Unreleased Structures...

2017-09-27T14:53:20Z

New page: '''Unreleased structure''' The entry 5wj9 is ON HOLD until Paper Publication Authors: Schmiege, P., Li, X. Description: cryo-EM of a lysosomal protein [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 5wj9 is ON HOLD until Paper Publication

Authors: Schmiege, P., Li, X.

Description: cryo-EM of a lysosomal protein
[[Category: Unreleased Structures]]
[[Category: Schmiege, P]]
[[Category: Li, X]]