5wm9 - Revision history

OCA at 14:16, 13 March 2024

2024-03-13T14:16:29Z

←Older revision		Revision as of 14:16, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='5wm9' size='340' side='right'caption='[[5wm9]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='5wm9' size='340' side='right'caption='[[5wm9]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wm9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WM9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wm9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WM9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8500029Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Rv0078 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83332 MYCTU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm9 OCA], [~~http~~://pdbe.org/5wm9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wm9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wm9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm9 OCA], [https://pdbe.org/5wm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wm9 RCSB], [https://www.ebi.ac.uk/pdbsum/5wm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm9 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/O53623_MYCTU O53623_MYCTU]
-	It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. M. tuberculosis is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of M. tuberculosis While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect M. tuberculosis acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae.IMPORTANCE Cytokinins have only previously been known as plant hormones. ~~The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life~~.	+

-	Cytokinin Signaling in Mycobacterium tuberculosis.,Samanovic MI, Hsu HC, Jones MB, Jones V, McNeil MR, Becker SH, Jordan AT, Strnad M, Xu C, Jackson M, Li H, Darwin KH MBio. 2018 Jun 19;9(3). pii: mBio.00989-18. doi: 10.1128/mBio.00989-18. PMID:29921668<ref>PMID:29921668</ref>	+	==See Also==
-		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5wm9" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Hsu~~, H C~~]]	+	[[Category: Hsu HC]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Rv0078]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 06:31, 17 April 2019

2019-04-17T06:31:57Z

←Older revision		Revision as of 06:31, 17 April 2019
Line 1:		Line 1:

	==Crystal Structure of TetR family regulator Rv0078 from Mycobacterium tuberculosis==		==Crystal Structure of TetR family regulator Rv0078 from Mycobacterium tuberculosis==
-	<StructureSection load='5wm9' size='340' side='right' caption='[[5wm9]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='5wm9' size='340' side='right'caption='[[5wm9]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wm9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WM9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wm9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WM9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv0078 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm9 OCA], [http://pdbe.org/5wm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wm9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm9 OCA], [http://pdbe.org/5wm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wm9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm9 ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Hsu, H C]]		[[Category: Hsu, H C]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 07:05, 4 July 2018

2018-07-04T07:05:14Z

←Older revision		Revision as of 07:05, 4 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wm9 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of TetR family regulator Rv0078 from Mycobacterium tuberculosis==
		+	<StructureSection load='5wm9' size='340' side='right' caption='[[5wm9]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wm9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WM9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wm9 OCA], [http://pdbe.org/5wm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wm9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wm9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. M. tuberculosis is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of M. tuberculosis While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect M. tuberculosis acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae.IMPORTANCE Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.

-	~~Authors:~~ Hsu, H.C., Li, H.	+	Cytokinin Signaling in Mycobacterium tuberculosis.,Samanovic MI, Hsu HC, Jones MB, Jones V, McNeil MR, Becker SH, Jordan AT, Strnad M, Xu C, Jackson M, Li H, Darwin KH MBio. 2018 Jun 19;9(3). pii: mBio.00989-18. doi: 10.1128/mBio.00989-18. PMID:29921668<ref>PMID:29921668</ref>

-	~~Description: Crystal Structure~~ of ~~TetR family regulator Rv0078 from Mycobacterium tuberculosis~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Hsu, H.C]]	+	<div class="pdbe-citations 5wm9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hsu, H C]]
	[[Category: Li, H]]		[[Category: Li, H]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Rv0078]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA: Protected "5wm9" [edit=sysop:move=sysop]

2017-09-27T14:58:42Z

Protected "5wm9" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:58, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5wm9 is ON HOLD until Paper Publication Authors: Hsu, H.C., Li, H. Description: Crystal Structure of TetR family regulator Rv0078 from Mycobacteriu...

2017-09-27T14:58:41Z

New page: '''Unreleased structure''' The entry 5wm9 is ON HOLD until Paper Publication Authors: Hsu, H.C., Li, H. Description: Crystal Structure of TetR family regulator Rv0078 from Mycobacteriu...

New page

'''Unreleased structure'''

The entry 5wm9 is ON HOLD until Paper Publication

Authors: Hsu, H.C., Li, H.

Description: Crystal Structure of TetR family regulator Rv0078 from Mycobacterium tuberculosis
[[Category: Unreleased Structures]]
[[Category: Hsu, H.C]]
[[Category: Li, H]]