5wpz - Revision history

OCA at 16:51, 8 November 2023

2023-11-08T16:51:03Z

←Older revision		Revision as of 16:51, 8 November 2023
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	<StructureSection load='5wpz' size='340' side='right'caption='[[5wpz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5wpz' size='340' side='right'caption='[[5wpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wpz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WPZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wpz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WPZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5h7n\|5h7n]]~~, ~~[[5h7q\|5h7q]], [[5wq6\|5wq6]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wpz OCA], [~~http~~://pdbe.org/5wpz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wpz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wpz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wpz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wpz OCA], [https://pdbe.org/5wpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wpz RCSB], [https://www.ebi.ac.uk/pdbsum/5wpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wpz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/MNDA_HUMAN MNDA_HUMAN] May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jin~~, T C~~]]	+	[[Category: Jin TC]]
-	[[Category: Xiao~~, T S]]~~	+	[[Category: Xiao TS]]
-	~~[[Category: Crystallization tag]]~~	+
-	~~[[Category: Death fold]]~~	+
-	~~[[Category: Mbp tag]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 07:14, 7 October 2020

2020-10-07T07:14:08Z

←Older revision		Revision as of 07:14, 7 October 2020
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	==Crystal structure of MNDA PYD with MBP tag==		==Crystal structure of MNDA PYD with MBP tag==
-	<StructureSection load='5wpz' size='340' side='right' caption='[[5wpz]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5wpz' size='340' side='right'caption='[[5wpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wpz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WPZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wpz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WPZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WPZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MAL~~:~~MALTOSE~~'>~~MAL~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wq6\|5wq6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wq6\|5wq6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wpz OCA], [http://pdbe.org/5wpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wpz RCSB], [http://www.ebi.ac.uk/pdbsum/5wpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wpz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wpz OCA], [http://pdbe.org/5wpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wpz RCSB], [http://www.ebi.ac.uk/pdbsum/5wpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wpz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Jin, T C]]		[[Category: Jin, T C]]
	[[Category: Xiao, T S]]		[[Category: Xiao, T S]]

OCA at 17:47, 10 March 2017

2017-03-10T17:47:53Z

←Older revision		Revision as of 17:47, 10 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5wpz is ~~ON HOLD~~	+	==Crystal structure of MNDA PYD with MBP tag==
		+	<StructureSection load='5wpz' size='340' side='right' caption='[[5wpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wpz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WPZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h7n\|5h7n]], [[5h7q\|5h7q]], [[5wq6\|5wq6]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wpz OCA], [http://pdbe.org/5wpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wpz RCSB], [http://www.ebi.ac.uk/pdbsum/5wpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wpz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallization chaperones have been used to facilitate the crystallization of challenging proteins. Even though the maltose-binding protein (MBP) is one of the most commonly used crystallization chaperones, the design of optimal expression constructs for crystallization of MBP fusion proteins remains a challenge. To increase the success rate of MBP-facilitated crystallization, a series of expression vectors have been designed with either a short flexible linker or a set of rigid helical linkers. Seven death domain superfamily members were tested for crystallization with this set of vectors, six of which had never been crystallized before. All of the seven targets were crystallized, and their structures were determined using at least one of the vectors. Our successful crystallization of all of the targets demonstrates the validity of our approach and expands the arsenal of the crystallization chaperone toolkit, which may be applicable to crystallization of other difficult protein targets, as well as to other crystallization chaperones.

-	~~Authors: Jin~~, T~~.C.~~, ~~Xiao~~, T.S.	+	Design of an expression system to enhance MBP-mediated crystallization.,Jin T, Chuenchor W, Jiang J, Cheng J, Li Y, Fang K, Huang M, Smith P, Xiao TS Sci Rep. 2017 Jan 23;7:40991. doi: 10.1038/srep40991. PMID:28112203<ref>PMID:28112203</ref>

-	~~Description: Crystal structure~~ of ~~MNDA PYD with MBP tag~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Jin, T.C]]	+	<div class="pdbe-citations 5wpz" style="background-color:#fffaf0;"></div>
-	[[Category: Xiao, T.S]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jin, T C]]
		+	[[Category: Xiao, T S]]
		+	[[Category: Crystallization tag]]
		+	[[Category: Death fold]]
		+	[[Category: Mbp tag]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "5wpz" [edit=sysop:move=sysop]

2016-12-09T18:16:17Z

Protected "5wpz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:16, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wpz is ON HOLD Authors: Jin, T.C., Xiao, T.S. Description: Crystal structure of MNDA PYD with MBP tag Category: Unreleased Structures [[Catego...

2016-12-09T18:16:16Z

New page: '''Unreleased structure''' The entry 5wpz is ON HOLD Authors: Jin, T.C., Xiao, T.S. Description: Crystal structure of MNDA PYD with MBP tag Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 5wpz is ON HOLD

Authors: Jin, T.C., Xiao, T.S.

Description: Crystal structure of MNDA PYD with MBP tag
[[Category: Unreleased Structures]]
[[Category: Jin, T.C]]
[[Category: Xiao, T.S]]