5wtw - Revision history

OCA at 16:53, 8 November 2023

2023-11-08T16:53:55Z

←Older revision		Revision as of 16:53, 8 November 2023
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	<StructureSection load='5wtw' size='340' side='right'caption='[[5wtw]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='5wtw' size='340' side='right'caption='[[5wtw]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wtw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hbv Hbv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wtw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_B_virus Hepatitis B virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.623Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtw OCA], [~~http~~://pdbe.org/5wtw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wtw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wtw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtw ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtw OCA], [https://pdbe.org/5wtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtw RCSB], [https://www.ebi.ac.uk/pdbsum/5wtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CAPSD_HBVD1 CAPSD_HBVD1] Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions (By similarity).<ref>PMID:7711014</ref> Encapsidates hepatitis delta genome (By similarity).<ref>PMID:7711014</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hbv~~]]	+	[[Category: Hepatitis B virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Xu~~, Z H~~]]	+	[[Category: Xu ZH]]
-	[[Category: Zhou, Z]]	+	[[Category: Zhou Z]]
-	~~[[Category: Capsid]]~~	+
-	~~[[Category: Core protein]]~~	+
-	~~[[Category: Hepatitis b virus]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Y132a dimer]]~~	+
-	~~[[Category: Y132a hexamer~~]]	+

OCA at 10:02, 1 January 2020

2020-01-01T10:02:18Z

←Older revision		Revision as of 10:02, 1 January 2020
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	==Hepatitis B virus core protein Y132A mutant in P 41 21 2 Space Group==		==Hepatitis B virus core protein Y132A mutant in P 41 21 2 Space Group==
-	<StructureSection load='5wtw' size='340' side='right' caption='[[5wtw]], [[Resolution\|resolution]] 2.62Å' scene=''>	+	<StructureSection load='5wtw' size='340' side='right'caption='[[5wtw]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wtw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wtw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hbv Hbv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtw OCA], [http://pdbe.org/5wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtw RCSB], [http://www.ebi.ac.uk/pdbsum/5wtw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtw OCA], [http://pdbe.org/5wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtw RCSB], [http://www.ebi.ac.uk/pdbsum/5wtw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heteroaryldihydropyrimidine (HAP) and sulfamoylbenzamide (SBA) are promising non-nucleos(t)ide HBV replication inhibitors. HAPs are known to promote core protein mis-assembly, but the molecular mechanism of abnormal assembly is still elusive. Likewise, the assembly status of core protein induced by SBA remains unknown. Here we show that SBA, unlike HAP, does not promote core protein mis-assembly. Interestingly, two reference compounds HAP_R01 and SBA_R01 bind to the same pocket at the dimer-dimer interface in the crystal structures of core protein Y132A hexamer. The striking difference lies in a unique hydrophobic subpocket that is occupied by the thiazole group of HAP_R01, but is unperturbed by SBA_R01. Photoaffinity labeling confirms the HAP_R01 binding pose at the dimer-dimer interface on capsid and suggests a new mechanism of HAP-induced mis-assembly. Based on the common features in crystal structures we predict that T33 mutations generate similar susceptibility changes to both compounds. In contrast, mutations at positions in close contact with HAP-specific groups (P25A, P25S, or V124F) only reduce susceptibility to HAP_R01, but not to SBA_R01. Thus, HAP and SBA are likely to have distinctive resistance profiles. Notably, P25S and V124F substitutions exist in low-abundance quasispecies in treatment-naive patients, suggesting potential clinical relevance.
		+
		+	Heteroaryldihydropyrimidine (HAP) and Sulfamoylbenzamide (SBA) Inhibit Hepatitis B Virus Replication by Different Molecular Mechanisms.,Zhou Z, Hu T, Zhou X, Wildum S, Garcia-Alcalde F, Xu Z, Wu D, Mao Y, Tian X, Zhou Y, Shen F, Zhang Z, Tang G, Najera I, Yang G, Shen HC, Young JA, Qin N Sci Rep. 2017 Feb 13;7:42374. doi: 10.1038/srep42374. PMID:28205569<ref>PMID:28205569</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wtw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hbv]]
		+	[[Category: Large Structures]]
	[[Category: Xu, Z H]]		[[Category: Xu, Z H]]
	[[Category: Zhou, Z]]		[[Category: Zhou, Z]]
	[[Category: Capsid]]		[[Category: Capsid]]
	[[Category: Core protein]]		[[Category: Core protein]]
-	[[Category: Hbv]]
	[[Category: Hepatitis b virus]]		[[Category: Hepatitis b virus]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
	[[Category: Y132a dimer]]		[[Category: Y132a dimer]]
	[[Category: Y132a hexamer]]		[[Category: Y132a hexamer]]

OCA at 01:46, 11 March 2017

2017-03-11T01:46:20Z

←Older revision		Revision as of 01:46, 11 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5wtw is ~~ON HOLD~~	+	==Hepatitis B virus core protein Y132A mutant in P 41 21 2 Space Group==
-		+	<StructureSection load='5wtw' size='340' side='right' caption='[[5wtw]], [[Resolution\|resolution]] 2.62Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5wtw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTW FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtw OCA], [http://pdbe.org/5wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtw RCSB], [http://www.ebi.ac.uk/pdbsum/5wtw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtw ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Xu, Z H]]
		+	[[Category: Zhou, Z]]
		+	[[Category: Capsid]]
		+	[[Category: Core protein]]
		+	[[Category: Hbv]]
		+	[[Category: Hepatitis b virus]]
		+	[[Category: Viral protein]]
		+	[[Category: Y132a dimer]]
		+	[[Category: Y132a hexamer]]

OCA: Protected "5wtw" [edit=sysop:move=sysop]

2016-12-22T20:42:37Z

Protected "5wtw" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:42, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5wtw is ON HOLD Authors: Description: Category: Unreleased Structures

2016-12-22T20:42:36Z

New page: '''Unreleased structure''' The entry 5wtw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5wtw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]