5wwk - Revision history

OCA at 07:49, 22 November 2023

2023-11-22T07:49:57Z

←Older revision		Revision as of 07:49, 22 November 2023
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	<StructureSection load='5wwk' size='340' side='right'caption='[[5wwk]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5wwk' size='340' side='right'caption='[[5wwk]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wwk]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wwk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WWK FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.199Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwk OCA], [~~http~~://pdbe.org/5wwk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wwk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wwk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwk ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwk OCA], [https://pdbe.org/5wwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wwk RCSB], [https://www.ebi.ac.uk/pdbsum/5wwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A059PIQ0_AEQVI A0A059PIQ0_AEQVI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ayyadurai, N]]	+	[[Category: Ayyadurai N]]
-	[[Category: Easwaramoorthi, S]]	+	[[Category: Easwaramoorthi S]]
-	[[Category: Ganesh, S]]	+	[[Category: Ganesh S]]
-	[[Category: George, A]]	+	[[Category: George A]]
-	[[Category: Gunasekaran, K]]	+	[[Category: Gunasekaran K]]
-	[[Category: Jaimohan~~, S M~~]]	+	[[Category: Jaimohan SM]]
-	[[Category: Kamini~~, N R~~]]	+	[[Category: Kamini NR]]
-	[[Category: Kesavan, M]]	+	[[Category: Kesavan M]]
-	[[Category: Sriram, R]]	+	[[Category: Sriram R]]
-	~~[[Category: Chromophore modification]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Thermal stability fluorescence protein sensor~~]]	+

OCA at 17:14, 14 August 2019

2019-08-14T17:14:08Z

←Older revision		Revision as of 17:14, 14 August 2019
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	==Highly stable green fluorescent protein==		==Highly stable green fluorescent protein==
-	<StructureSection load='5wwk' size='340' side='right' caption='[[5wwk]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5wwk' size='340' side='right'caption='[[5wwk]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wwk]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wwk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWK FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwk OCA], [http://pdbe.org/5wwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wwk RCSB], [http://www.ebi.ac.uk/pdbsum/5wwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwk OCA], [http://pdbe.org/5wwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wwk RCSB], [http://www.ebi.ac.uk/pdbsum/5wwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwk ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Red fluorescent proteins with a large Stokes shift offer a limited autofluorescence background and are used in deep tissue imaging. Here, by introducing the free amino group in Aequorea victoria, the electrostatic charges of the p-hydroxybenzylidene imidazolinone chromophore of green fluorescent protein (GFP) have been altered resulting in an unusual, 85 nm red-shifted fluorescence. The structural and biophysical analysis suggested that the red shift is due to positional shift occupancy of Glu222 and Arg96, resulting in extended conjugation and a relaxed chromophore. Femtosecond transient absorption spectra exhibited that the excited state relaxation dynamics of red-shifted GFP (rGFP) (tau4 = 234 ps) are faster compared to the A. victoria green fluorescent protein (tau4 = 3.0 ns). The nanosecond time-resolved emission spectra of rGFP reveal the continuous spectral shift during emission by a solvent reorientation in the chromophore. Finally, the molecular dynamics simulations revealed the rearrangement of the hydrogen bond interactions in the chromophore vicinity, reshaping the symmetric distribution of van der Waals space to fine tune the GFP structure resulting from highly red-shifted rGFP.
		+
		+	Excited State Electronic Interconversion and Structural Transformation of Engineered Red-Emitting Green Fluorescent Protein Mutant.,Augustine G, Raghavan S, NumbiRamudu K, Easwaramoorthi S, Shanmugam G, Seetharani Murugaiyan J, Gunasekaran K, Govind C, Karunakaran V, Ayyadurai N J Phys Chem B. 2019 Mar 14;123(10):2316-2324. doi: 10.1021/acs.jpcb.8b10516. Epub, 2019 Mar 6. PMID:30789731<ref>PMID:30789731</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5wwk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Ayyadurai, N]]		[[Category: Ayyadurai, N]]
	[[Category: Easwaramoorthi, S]]		[[Category: Easwaramoorthi, S]]

OCA at 07:00, 13 December 2017

2017-12-13T07:00:17Z

←Older revision		Revision as of 07:00, 13 December 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5wwk ~~is ON HOLD until Paper Publication~~	+	==Highly stable green fluorescent protein==
-		+	<StructureSection load='5wwk' size='340' side='right' caption='[[5wwk]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~: ~~Sriram, R~~.~~, George, A~~.~~, Kesavan, M~~., ~~Jaimohan, S~~.M., ~~Kamini, N~~.R., ~~Easwaramoorthi, S~~., ~~Ganesh, S~~., ~~Gunasekaran, K~~., ~~Ayyadurai, N~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5wwk]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WWK FirstGlance]. <br>
-	~~Description: Highly stable green fluorescent protein~~	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
-	~~[[Category: Unreleased Structures]]~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wwk OCA], [http://pdbe.org/5wwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wwk RCSB], [http://www.ebi.ac.uk/pdbsum/5wwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wwk ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ayyadurai, N]]		[[Category: Ayyadurai, N]]
-	[[Category: Kamini, N.R]]
-	[[Category: Sriram, R]]
	[[Category: Easwaramoorthi, S]]		[[Category: Easwaramoorthi, S]]
	[[Category: Ganesh, S]]		[[Category: Ganesh, S]]
		+	[[Category: George, A]]
	[[Category: Gunasekaran, K]]		[[Category: Gunasekaran, K]]
		+	[[Category: Jaimohan, S M]]
		+	[[Category: Kamini, N R]]
	[[Category: Kesavan, M]]		[[Category: Kesavan, M]]
-	[[Category: ~~Jaimohan~~, ~~S.M~~]]	+	[[Category: Sriram, R]]
-	[[Category: ~~George, A~~]]	+	[[Category: Chromophore modification]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Thermal stability fluorescence protein sensor]]

OCA at 15:09, 27 September 2017

2017-09-27T15:09:49Z

←Older revision		Revision as of 15:09, 27 September 2017
Line 7:		Line 7:
	Description: Highly stable green fluorescent protein		Description: Highly stable green fluorescent protein
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Easwaramoorthi, S]]
	[[Category: Ayyadurai, N]]		[[Category: Ayyadurai, N]]
		+	[[Category: Kamini, N.R]]
		+	[[Category: Sriram, R]]
		+	[[Category: Easwaramoorthi, S]]
	[[Category: Ganesh, S]]		[[Category: Ganesh, S]]
	[[Category: Gunasekaran, K]]		[[Category: Gunasekaran, K]]
-	[[Category: Kamini, N.R]]
	[[Category: Kesavan, M]]		[[Category: Kesavan, M]]
-	[[Category: Sriram, R]]
	[[Category: Jaimohan, S.M]]		[[Category: Jaimohan, S.M]]
	[[Category: George, A]]		[[Category: George, A]]

OCA at 19:55, 15 March 2017

2017-03-15T19:55:57Z

←Older revision		Revision as of 19:55, 15 March 2017
Line 7:		Line 7:
	Description: Highly stable green fluorescent protein		Description: Highly stable green fluorescent protein
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ayyadurai, N]]
-	[[Category: Kamini, N.R]]
-	[[Category: Sriram, R]]
	[[Category: Easwaramoorthi, S]]		[[Category: Easwaramoorthi, S]]
		+	[[Category: Ayyadurai, N]]
	[[Category: Ganesh, S]]		[[Category: Ganesh, S]]
	[[Category: Gunasekaran, K]]		[[Category: Gunasekaran, K]]
		+	[[Category: Kamini, N.R]]
	[[Category: Kesavan, M]]		[[Category: Kesavan, M]]
		+	[[Category: Sriram, R]]
	[[Category: Jaimohan, S.M]]		[[Category: Jaimohan, S.M]]
	[[Category: George, A]]		[[Category: George, A]]

OCA at 15:25, 9 March 2017

2017-03-09T15:25:28Z

←Older revision		Revision as of 15:25, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5wwk is ON HOLD	+	The entry 5wwk is ON HOLD until Paper Publication

	Authors: Sriram, R., George, A., Kesavan, M., Jaimohan, S.M., Kamini, N.R., Easwaramoorthi, S., Ganesh, S., Gunasekaran, K., Ayyadurai, N.		Authors: Sriram, R., George, A., Kesavan, M., Jaimohan, S.M., Kamini, N.R., Easwaramoorthi, S., Ganesh, S., Gunasekaran, K., Ayyadurai, N.

OCA: Protected "5wwk" [edit=sysop:move=sysop]

2017-01-18T15:16:39Z

Protected "5wwk" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:16, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5wwk is ON HOLD Authors: Sriram, R., George, A., Kesavan, M., Jaimohan, S.M., Kamini, N.R., Easwaramoorthi, S., Ganesh, S., Gunasekaran, K., Ayyadur...

2017-01-18T15:16:38Z

New page: '''Unreleased structure''' The entry 5wwk is ON HOLD Authors: Sriram, R., George, A., Kesavan, M., Jaimohan, S.M., Kamini, N.R., Easwaramoorthi, S., Ganesh, S., Gunasekaran, K., Ayyadur...

New page

'''Unreleased structure'''

The entry 5wwk is ON HOLD

Authors: Sriram, R., George, A., Kesavan, M., Jaimohan, S.M., Kamini, N.R., Easwaramoorthi, S., Ganesh, S., Gunasekaran, K., Ayyadurai, N.

Description: Highly stable green fluorescent protein
[[Category: Unreleased Structures]]
[[Category: Ayyadurai, N]]
[[Category: Kamini, N.R]]
[[Category: Sriram, R]]
[[Category: Easwaramoorthi, S]]
[[Category: Ganesh, S]]
[[Category: Gunasekaran, K]]
[[Category: Kesavan, M]]
[[Category: Jaimohan, S.M]]
[[Category: George, A]]