5wy3 - Revision history

OCA at 09:19, 20 March 2024

2024-03-20T09:19:20Z

←Older revision		Revision as of 09:19, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='5wy3' size='340' side='right'caption='[[5wy3]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5wy3' size='340' side='right'caption='[[5wy3]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5WY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wy3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WY3 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~5wy4~~\|~~5wy4~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5wy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy3 OCA], [~~http~~://pdbe.org/5wy3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5wy3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5wy3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy3 OCA], [https://pdbe.org/5wy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wy3 RCSB], [https://www.ebi.ac.uk/pdbsum/5wy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy3 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/G0S5L1_CHATD G0S5L1_CHATD]
-	Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.	+
-		+
-	~~Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii~~: ~~e22086. doi: 10.7554~~/~~eLife.22086. PMID:28244370<ref>PMID:28244370<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5wy3" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, R]]	+	[[Category: Chen R]]
-	[[Category: Ye, K]]	+	[[Category: Ye K]]
-	[[Category: Zhu, X]]	+	[[Category: Zhu X]]
-	~~[[Category: Components of 90s preribosome]]~~	+
-	~~[[Category: Nuclear protein]]~~	+
-	~~[[Category: Nucleolar protein]]~~	+
-	~~[[Category: Ribosomal protein~~]]	+

OCA at 07:19, 7 October 2020

2020-10-07T07:19:42Z

←Older revision		Revision as of 07:19, 7 October 2020
Line 1:		Line 1:

	==Crystal structure of Chaetomium thermophilum Utp10 middle domain==		==Crystal structure of Chaetomium thermophilum Utp10 middle domain==
-	<StructureSection load='5wy3' size='340' side='right' caption='[[5wy3]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5wy3' size='340' side='right'caption='[[5wy3]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5wy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5WY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5wy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WY3 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wy4\|5wy4]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wy4\|5wy4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5wy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy3 OCA], [http://pdbe.org/5wy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5wy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy3 OCA], [http://pdbe.org/5wy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5wy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chen, R]]		[[Category: Chen, R]]
	[[Category: Ye, K]]		[[Category: Ye, K]]

OCA at 03:59, 30 August 2017

2017-08-30T03:59:14Z

←Older revision		Revision as of 03:59, 30 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5wy3 is ~~ON HOLD until Jan 10 2019~~	+	==Crystal structure of Chaetomium thermophilum Utp10 middle domain==
		+	<StructureSection load='5wy3' size='340' side='right' caption='[[5wy3]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5wy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WY3 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wy4\|5wy4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy3 OCA], [http://pdbe.org/5wy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5wy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.

-	~~Authors: Chen, R~~., Zhu, X., Ye, K.	+	Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370<ref>PMID:28244370</ref>

-	~~Description: Crystal structure~~ of ~~Chaetomium thermophilum Utp10 middle domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5wy3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, R]]
	[[Category: Ye, K]]		[[Category: Ye, K]]
	[[Category: Zhu, X]]		[[Category: Zhu, X]]
-	[[Category: ~~Chen, R~~]]	+	[[Category: Components of 90s preribosome]]
		+	[[Category: Nuclear protein]]
		+	[[Category: Nucleolar protein]]
		+	[[Category: Ribosomal protein]]

OCA at 19:58, 15 March 2017

2017-03-15T19:58:37Z

←Older revision		Revision as of 19:58, 15 March 2017
Line 7:		Line 7:
	Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain		Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Chen, R]]
	[[Category: Ye, K]]		[[Category: Ye, K]]
	[[Category: Zhu, X]]		[[Category: Zhu, X]]
		+	[[Category: Chen, R]]

OCA at 22:34, 25 January 2017

2017-01-25T22:34:23Z

←Older revision		Revision as of 22:34, 25 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5wy3 is ON HOLD	+	The entry 5wy3 is ON HOLD until Jan 10 2019

-	Authors: Ye, K., ~~Chen~~, R.	+	Authors: Chen, R., Zhu, X., Ye, K.

	Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain		Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain
Line 9:		Line 9:
	[[Category: Chen, R]]		[[Category: Chen, R]]
	[[Category: Ye, K]]		[[Category: Ye, K]]
		+	[[Category: Zhu, X]]

OCA: Protected "5wy3" [edit=sysop:move=sysop]

2017-01-18T15:20:39Z

Protected "5wy3" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:20, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5wy3 is ON HOLD Authors: Ye, K., Chen, R. Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain [[Category: Unreleased Stru...

2017-01-18T15:20:37Z

New page: '''Unreleased structure''' The entry 5wy3 is ON HOLD Authors: Ye, K., Chen, R. Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 5wy3 is ON HOLD

Authors: Ye, K., Chen, R.

Description: Crystal structure of Chaetomium thermophilum Utp10 middle domain
[[Category: Unreleased Structures]]
[[Category: Chen, R]]
[[Category: Ye, K]]