5x2e - Revision history

OCA at 10:16, 27 March 2024

2024-03-27T10:16:53Z

←Older revision		Revision as of 10:16, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='5x2e' size='340' side='right'caption='[[5x2e]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='5x2e' size='340' side='right'caption='[[5x2e]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5X2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clonorchis_sinensis Clonorchis sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X2E FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.299Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5x2d\|5x2d]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5x2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2e OCA], [~~http~~://pdbe.org/5x2e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5x2e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5x2e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2e OCA], [https://pdbe.org/5x2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x2e RCSB], [https://www.ebi.ac.uk/pdbsum/5x2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2e ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q2PMV7_CLOSI Q2PMV7_CLOSI]
-	Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (DLC)-like domain (aa 83-177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3). The calmodulin like domain has two Ca2+-binding sites (named CB1 and CB2), but Ca2+ binds to only one site, CB1. The DLC-like domain has a dimeric conformation; the interface is formed mainly by hydrogen bonds between the main chain atoms. In addition, we have determined full-length structure of CsTAL3 in solution and showed the conformational change of CsTAL3 induced by Ca2+ ion binding using small-angle X-ray scattering analysis and molecular dynamics simulations. The Ca2+-bound form has a more extended conformation than the Ca2+-free from does. These structural and biochemical analyses will advance the understanding of the biology of this liver fluke and may contribute to our understanding of the molecular mechanism of calcium-responsive and tegumental-allergen-like proteins.	+
-		+
-	Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis.,Jo CH, Son J, Kim S, Oda T, Kim J, Lee MR, Sato M, Kim HT, Unzai S, Park SY, Hwang KY Sci Rep. 2017 May 11;7(1):~~1764. doi: 10.1038~~/~~s41598-017-02044-0. PMID:28496122<ref>PMID:28496122</ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5x2e" style="background-color:#fffaf0;"><~~/~~div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Large Structures]]
-	[[Category: Hwang, K Y]]
-	[[Category: Jo, C H]]
-	[[Category: Calcium-binding protein]]
-	[[Category: Calmodulin]]
-	[[Category: Cell adhesion]]
	[[Category: Clonorchis sinensis]]		[[Category: Clonorchis sinensis]]
-	[[Category: ~~Cstal3~~]]	+	[[Category: Large Structures]]
		+	[[Category: Hwang KY]]
		+	[[Category: Jo CH]]

OCA at 07:22, 7 October 2020

2020-10-07T07:22:19Z

←Older revision		Revision as of 07:22, 7 October 2020
Line 1:		Line 1:

	==Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa)==		==Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa)==
-	<StructureSection load='5x2e' size='340' side='right' caption='[[5x2e]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='5x2e' size='340' side='right'caption='[[5x2e]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5x2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5X2E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5x2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X2E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x2d\|5x2d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x2d\|5x2d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5x2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2e OCA], [http://pdbe.org/5x2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x2e RCSB], [http://www.ebi.ac.uk/pdbsum/5x2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2e OCA], [http://pdbe.org/5x2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x2e RCSB], [http://www.ebi.ac.uk/pdbsum/5x2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2e ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hwang, K Y]]		[[Category: Hwang, K Y]]
	[[Category: Jo, C H]]		[[Category: Jo, C H]]

OCA at 04:07, 30 August 2017

2017-08-30T04:07:29Z

←Older revision		Revision as of 04:07, 30 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5x2e is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa)==
		+	<StructureSection load='5x2e' size='340' side='right' caption='[[5x2e]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5x2e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X2E FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5x2d\|5x2d]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2e OCA], [http://pdbe.org/5x2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x2e RCSB], [http://www.ebi.ac.uk/pdbsum/5x2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2e ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (DLC)-like domain (aa 83-177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3). The calmodulin like domain has two Ca2+-binding sites (named CB1 and CB2), but Ca2+ binds to only one site, CB1. The DLC-like domain has a dimeric conformation; the interface is formed mainly by hydrogen bonds between the main chain atoms. In addition, we have determined full-length structure of CsTAL3 in solution and showed the conformational change of CsTAL3 induced by Ca2+ ion binding using small-angle X-ray scattering analysis and molecular dynamics simulations. The Ca2+-bound form has a more extended conformation than the Ca2+-free from does. These structural and biochemical analyses will advance the understanding of the biology of this liver fluke and may contribute to our understanding of the molecular mechanism of calcium-responsive and tegumental-allergen-like proteins.

-	~~Authors: Jo, C~~.H., ~~Hwang~~, K.Y.	+	Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis.,Jo CH, Son J, Kim S, Oda T, Kim J, Lee MR, Sato M, Kim HT, Unzai S, Park SY, Hwang KY Sci Rep. 2017 May 11;7(1):1764. doi: 10.1038/s41598-017-02044-0. PMID:28496122<ref>PMID:28496122</ref>

-	~~Description: Crystal structure~~ of ~~Calmodulin like domain~~ of ~~CsTAL3 (1-81aa)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Hwang, K.Y]]	+	<div class="pdbe-citations 5x2e" style="background-color:#fffaf0;"></div>
-	[[Category: Jo, C.H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hwang, K Y]]
		+	[[Category: Jo, C H]]
		+	[[Category: Calcium-binding protein]]
		+	[[Category: Calmodulin]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Clonorchis sinensis]]
		+	[[Category: Cstal3]]

OCA: Protected "5x2e" [edit=sysop:move=sysop]

2017-03-09T15:36:40Z

Protected "5x2e" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:36, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5x2e is ON HOLD until Paper Publication Authors: Jo, C.H., Hwang, K.Y. Description: Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa) ...

2017-03-09T15:36:38Z

New page: '''Unreleased structure''' The entry 5x2e is ON HOLD until Paper Publication Authors: Jo, C.H., Hwang, K.Y. Description: Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa) ...

New page

'''Unreleased structure'''

The entry 5x2e is ON HOLD until Paper Publication

Authors: Jo, C.H., Hwang, K.Y.

Description: Crystal structure of Calmodulin like domain of CsTAL3 (1-81aa)
[[Category: Unreleased Structures]]
[[Category: Hwang, K.Y]]
[[Category: Jo, C.H]]