5xbs - Revision history

OCA at 06:45, 14 October 2020

2020-10-14T06:45:47Z

←Older revision		Revision as of 06:45, 14 October 2020
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	==Peroxiredoxin from Aeropyrum pernix (6m mutant)==		==Peroxiredoxin from Aeropyrum pernix (6m mutant)==
-	<StructureSection load='5xbs' size='340' side='right' caption='[[5xbs]], [[Resolution\|resolution]] 2.51Å' scene=''>	+	<StructureSection load='5xbs' size='340' side='right'caption='[[5xbs]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xbs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XBS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xbs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XBS FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbq\|5xbq]], [[5xbr\|5xbr]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbq\|5xbq]], [[5xbr\|5xbr]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbs OCA], [http://pdbe.org/5xbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xbs RCSB], [http://www.ebi.ac.uk/pdbsum/5xbs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbs OCA], [http://pdbe.org/5xbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xbs RCSB], [http://www.ebi.ac.uk/pdbsum/5xbs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5xbs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xbs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
	[[Category: Nakamura, T]]		[[Category: Nakamura, T]]

OCA at 05:53, 31 January 2018

2018-01-31T05:53:57Z

←Older revision		Revision as of 05:53, 31 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5xbs is ~~ON HOLD until Paper~~ Publication	+	==Peroxiredoxin from Aeropyrum pernix (6m mutant)==
		+	<StructureSection load='5xbs' size='340' side='right' caption='[[5xbs]], [[Resolution\|resolution]] 2.51Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xbs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XBS FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbq\|5xbq]], [[5xbr\|5xbr]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbs OCA], [http://pdbe.org/5xbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xbs RCSB], [http://www.ebi.ac.uk/pdbsum/5xbs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbs ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peroxiredoxin from Pyrococcus horikoshii (PhPrx) is a decameric protein formed by ring-type assembly of five dimers. To engineer the quaternary structure of PhPrx, we created a mutant PhPrx (PhPrx6m) by introducing six point mutations designed to dissociate PhPrx into dimers. Although PhPrx6m was a dimer in solution, the six dimers assembled into a dodecamer following crystallization. In the crystal structure, PhPrx6m was overoxidized, and the peroxidatic cysteine was in sulfonic acid form and two cysteines in the C-terminal region were linked by an intramolecular disulfide bond. Thus, we characterized the wild-type PhPrx overoxidized by hydrogen peroxide (PhPrxPer). Analytical ultracentrifugation showed that PhPrxPer had a higher molecular mass in solution than PhPrx. This was confirmed by analysis of the crystal structure of PhPrxPer, which was found to form a ring-type dodecamer composed of six dimers. The monomeric structures of PhPrx6m and PhPrxPer differed from that of PhPrx in the relative orientation of two domains, reflecting the number of dimers in the ring-type assembly. Unlike PhPrx, homologous peroxiredoxin from Aeropyrum pernix (ApPrx) did not undergo hexameric association. This property can be explained by the stronger connection between the two domains in ApPrx due to its C-terminal extension relative to PhPrx.

-	~~Authors: Nakamura~~, T., ~~Uegaki~~, K.	+	Alteration of molecular assembly of peroxiredoxins from hyperthermophilic archaea.,Nakamura T, Oshima M, Yasuda M, Shimamura A, Morita J, Uegaki K J Biochem. 2017 Dec 1;162(6):415-422. doi: 10.1093/jb/mvx045. PMID:28992240<ref>PMID:28992240</ref>

-	~~Description: Peroxiredoxin from Aeropyrum pernix (6m mutant)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Uegaki, K~~]]	+	<div class="pdbe-citations 5xbs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Peroxiredoxin]]
	[[Category: Nakamura, T]]		[[Category: Nakamura, T]]
		+	[[Category: Uegaki, K]]
		+	[[Category: Dimer]]
		+	[[Category: Oxidoreductase]]

OCA at 10:36, 12 April 2017

2017-04-12T10:36:08Z

←Older revision		Revision as of 10:36, 12 April 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xbs is ON HOLD	+	The entry 5xbs is ON HOLD until Paper Publication

	Authors: Nakamura, T., Uegaki, K.		Authors: Nakamura, T., Uegaki, K.

OCA: Protected "5xbs" [edit=sysop:move=sysop]

2017-04-05T12:27:52Z

Protected "5xbs" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:27, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5xbs is ON HOLD Authors: Nakamura, T., Uegaki, K. Description: Peroxiredoxin from Aeropyrum pernix (6m mutant) Category: Unreleased Structures ...

2017-04-05T12:27:51Z

New page: '''Unreleased structure''' The entry 5xbs is ON HOLD Authors: Nakamura, T., Uegaki, K. Description: Peroxiredoxin from Aeropyrum pernix (6m mutant) Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 5xbs is ON HOLD

Authors: Nakamura, T., Uegaki, K.

Description: Peroxiredoxin from Aeropyrum pernix (6m mutant)
[[Category: Unreleased Structures]]
[[Category: Uegaki, K]]
[[Category: Nakamura, T]]