5xc4 - Revision history

OCA at 07:59, 22 November 2023

2023-11-22T07:59:16Z

←Older revision		Revision as of 07:59, 22 November 2023
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	<StructureSection load='5xc4' size='340' side='right'caption='[[5xc4]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='5xc4' size='340' side='right'caption='[[5xc4]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ampullaria_crossean Ampullaria crossean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ampullaria_crossean Ampullaria crossean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.42Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbx\|5xbx]]~~, [[~~5xbu~~\|~~5xbu]], [[5xca\|5xca]], [[5xcb\|5xcb]], [[5xc9\|5xc9~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>EG27II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~228791 Ampullaria crossean])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [https://pdbe.org/5xc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [https://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [~~http~~://pdbe.org/5xc4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A7KMF0_9CAEN A7KMF0_9CAEN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Glucanase\|Glucanase]]	+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Ampullaria crossean]]		[[Category: Ampullaria crossean]]
-	[[Category: Cellulase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Iwase, H]]	+	[[Category: Iwase H]]
-	[[Category: Mikami, B]]	+	[[Category: Mikami B]]
-	[[Category: Mizutani, K]]	+	[[Category: Mizutani K]]
-	[[Category: Nomura, T]]	+	[[Category: Nomura T]]
-	[[Category: Takahashi, N]]	+	[[Category: Takahashi N]]
-	~~[[Category: Glycoside hydrolase family 45]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:42, 24 April 2019

2019-04-24T07:42:47Z

←Older revision		Revision as of 07:42, 24 April 2019
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	==Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose==		==Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose==
-	<StructureSection load='5xc4' size='340' side='right' caption='[[5xc4]], [[Resolution\|resolution]] 1.42Å' scene=''>	+	<StructureSection load='5xc4' size='340' side='right'caption='[[5xc4]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ampullaria_crossean Ampullaria crossean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbx\|5xbx]], [[5xbu\|5xbu]], [[5xca\|5xca]], [[5xcb\|5xcb]], [[5xc9\|5xc9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbx\|5xbx]], [[5xbu\|5xbu]], [[5xca\|5xca]], [[5xcb\|5xcb]], [[5xc9\|5xc9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EG27II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228791 Ampullaria crossean])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [http://pdbe.org/5xc4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [http://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [http://pdbe.org/5xc4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [http://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although endogenous animal cellulases have great potential for industrial applications such as bioethanol production, few investigations have focused on these enzymes. In this study, the glycoside hydrolase family 45 (GH45) subfamily B endoglucanase EG27II from the snail Ampullaria crossean was expressed using a Pichia pastoris expression system and the crystal structure of the apo form was determined at 1.00 A resolution; this is the highest resolution structure of an animal endoglucanase. The results showed that EG27II has a double-psi six-stranded beta-barrel and that the structure of EG27II more closely resembles those of subfamily C enzymes than those of subfamily A enzymes. The structure of EG27II complexed with cellobiose was also determined under cryoconditions and at room temperature at three pH values, pH 4.0, 5.5 and 8.0, and no structural changes were found to be associated with the change in pH. The structural comparison and catalytic activity measurements showed that Asp137 and Asn112 function as the catalytic acid and base, respectively, and that Asp27 is also an important residue for catalysis. These high-resolution structures of EG27II provide a large amount of information for structure-based enzyme modification and cell-surface engineering, which will advance biofuel production using animal-derived cellulases.
		+
		+	High-resolution crystal structures of the glycoside hydrolase family 45 endoglucanase EG27II from the snail Ampullaria crossean.,Nomura T, Iwase H, Saka N, Takahashi N, Mikami B, Mizutani K Acta Crystallogr D Struct Biol. 2019 Apr 1;75(Pt 4):426-436. doi:, 10.1107/S2059798319003000. Epub 2019 Apr 4. PMID:30988259<ref>PMID:30988259</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xc4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase\|Glucanase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ampullaria crossean]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
		+	[[Category: Large Structures]]
	[[Category: Iwase, H]]		[[Category: Iwase, H]]
	[[Category: Mikami, B]]		[[Category: Mikami, B]]

OCA at 06:33, 28 March 2018

2018-03-28T06:33:23Z

←Older revision		Revision as of 06:33, 28 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5xc4 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose==
-		+	<StructureSection load='5xc4' size='340' side='right' caption='[[5xc4]], [[Resolution\|resolution]] 1.42Å' scene=''>
-	~~Authors~~: ~~Nomura~~, T., ~~Mizutani~~, K., ~~Iwase~~, H., ~~Takahashi~~, N., ~~Mikami~~, B.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5xc4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XC4 FirstGlance]. <br>
-	~~Description: Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xbx\|5xbx]], [[5xbu\|5xbu]], [[5xca\|5xca]], [[5xcb\|5xcb]], [[5xc9\|5xc9]]</td></tr>
-	[[Category: ~~Nomura~~, T]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xc4 OCA], [http://pdbe.org/5xc4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xc4 RCSB], [http://www.ebi.ac.uk/pdbsum/5xc4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xc4 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cellulase]]
		+	[[Category: Iwase, H]]
	[[Category: Mikami, B]]		[[Category: Mikami, B]]
-	[[Category: Iwase, H]]
	[[Category: Mizutani, K]]		[[Category: Mizutani, K]]
		+	[[Category: Nomura, T]]
	[[Category: Takahashi, N]]		[[Category: Takahashi, N]]
		+	[[Category: Glycoside hydrolase family 45]]
		+	[[Category: Hydrolase]]

OCA at 15:23, 27 September 2017

2017-09-27T15:23:13Z

←Older revision		Revision as of 15:23, 27 September 2017
Line 7:		Line 7:
	Description: Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose		Description: Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mizutani, K]]
	[[Category: Nomura, T]]		[[Category: Nomura, T]]
-	[[Category: Takahashi, N]]
	[[Category: Mikami, B]]		[[Category: Mikami, B]]
	[[Category: Iwase, H]]		[[Category: Iwase, H]]
		+	[[Category: Mizutani, K]]
		+	[[Category: Takahashi, N]]

OCA at 12:55, 27 April 2017

2017-04-27T12:55:20Z

←Older revision		Revision as of 12:55, 27 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xc4 is ON HOLD	+	The entry 5xc4 is ON HOLD until Paper Publication

	Authors: Nomura, T., Mizutani, K., Iwase, H., Takahashi, N., Mikami, B.		Authors: Nomura, T., Mizutani, K., Iwase, H., Takahashi, N., Mikami, B.

OCA: Protected "5xc4" [edit=sysop:move=sysop]

2017-04-05T12:28:31Z

Protected "5xc4" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:28, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5xc4 is ON HOLD Authors: Nomura, T., Mizutani, K., Iwase, H., Takahashi, N., Mikami, B. Description: Crystal structure of GH45 endoglucanase EG27II...

2017-04-05T12:28:30Z

New page: '''Unreleased structure''' The entry 5xc4 is ON HOLD Authors: Nomura, T., Mizutani, K., Iwase, H., Takahashi, N., Mikami, B. Description: Crystal structure of GH45 endoglucanase EG27II...

New page

'''Unreleased structure'''

The entry 5xc4 is ON HOLD

Authors: Nomura, T., Mizutani, K., Iwase, H., Takahashi, N., Mikami, B.

Description: Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose
[[Category: Unreleased Structures]]
[[Category: Mizutani, K]]
[[Category: Nomura, T]]
[[Category: Takahashi, N]]
[[Category: Mikami, B]]
[[Category: Iwase, H]]