5xoe - Revision history

OCA at 08:07, 22 November 2023

2023-11-22T08:07:55Z

←Older revision		Revision as of 08:07, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5xoe' size='340' side='right'caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='5xoe' size='340' side='right'caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staab Staab~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XOE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XOE FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pfkA, SAOUHSC_01807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=273036 STAAB])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.98Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6~~-~~phosphofructokinase 6-phosphofructokinase]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.~~7.1.11 2.7.1.11] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [https://pdbe.org/5xoe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [https://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [~~http~~://pdbe.org/5xoe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PFKA_STAA8 PFKA_STAA8]~~] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.~~[HAMAP-Rule:MF_00339]~~	+	[https://www.uniprot.org/uniprot/PFKA_STAAB PFKA_STAAB] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphofructokinase ~~(PFK)~~\|Phosphofructokinase ~~(PFK)~~]]	+	*[[Phosphofructokinase 3D structures\|Phosphofructokinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 6-phosphofructokinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staab~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-	[[Category: Tian, T]]	+	[[Category: Tian T]]
-	[[Category: Wang, C]]	+	[[Category: Wang C]]
-	[[Category: Zang, J]]	+	[[Category: Zang J]]
-	~~[[Category: Phosphofructokinase]]~~	+
-	~~[[Category: Staphylococcus aureus]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:23, 23 May 2019

2019-05-23T07:23:34Z

←Older revision		Revision as of 07:23, 23 May 2019
Line 1:		Line 1:

	==Crystal Structure of the apo Staphylococcus aureus phosphofructokinase==		==Crystal Structure of the apo Staphylococcus aureus phosphofructokinase==
-	<StructureSection load='5xoe' size='340' side='right' caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>	+	<StructureSection load='5xoe' size='340' side='right'caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOE FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pfkA, ~~SAB1557c~~ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pfkA, SAOUHSC_01807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [http://pdbe.org/5xoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [http://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [http://pdbe.org/5xoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [http://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~PFKA_STAAB PFKA_STAAB~~]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.	+	[[http://www.uniprot.org/uniprot/PFKA_STAA8 PFKA_STAA8]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_00339]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
		+	[[Category: Large Structures]]
	[[Category: Staab]]		[[Category: Staab]]
	[[Category: Tian, T]]		[[Category: Tian, T]]

OCA at 15:51, 27 February 2019

2019-02-27T15:51:37Z

←Older revision		Revision as of 15:51, 27 February 2019
Line 3:		Line 3:
	<StructureSection load='5xoe' size='340' side='right' caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='5xoe' size='340' side='right' caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staab Staab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOE FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pfkA, SAB1557c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273036 STAAB])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [http://pdbe.org/5xoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [http://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [http://pdbe.org/5xoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [http://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PFKA_STAAB PFKA_STAAB]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.		[[http://www.uniprot.org/uniprot/PFKA_STAAB PFKA_STAAB]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most reported bacterial phosphofructokinases (Pfks) are tetramers that exhibit activity allosterically regulated via conformational changes between the R and T states. We report that the Pfk from Staphylococcus aureus NCTC 8325 ( SaPfk) exists as both an active tetramer and an inactive dimer in solution. Multiple effectors, including pH, ADP, ATP, and adenylyl-imidodiphosphate (AMP-PNP), cause equilibrium shifts from the tetramer to dimer, whereas the substrate F6P stabilizes SaPfk tetrameric assembly. Crystal structures of SaPfk in complex with different ligands and biochemical analysis reveal that the flexibility of the Gly150-Leu151 motif in helix alpha7 plays a role in tetramer-dimer conversion. Thus, we propose a molecular mechanism for allosteric regulation of bacterial Pfk via conversion between the tetramer and dimer in addition to the well-characterized R-state/T-state mechanism.
		+
		+	Structural Insights into the Regulation of Staphylococcus aureus Phosphofructokinase by Tetramer-Dimer Conversion.,Tian T, Wang C, Wu M, Zhang X, Zang J Biochemistry. 2018 Jul 24;57(29):4252-4262. doi: 10.1021/acs.biochem.8b00028., Epub 2018 Jul 9. PMID:29940104<ref>PMID:29940104</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xoe" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
		+	[[Category: Staab]]
	[[Category: Tian, T]]		[[Category: Tian, T]]
	[[Category: Wang, C]]		[[Category: Wang, C]]

OCA at 05:41, 20 June 2018

2018-06-20T05:41:45Z

←Older revision		Revision as of 05:41, 20 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xoe is ~~ON HOLD until Jun 26 2019~~	+	==Crystal Structure of the apo Staphylococcus aureus phosphofructokinase==
-		+	<StructureSection load='5xoe' size='340' side='right' caption='[[5xoe]], [[Resolution\|resolution]] 2.98Å' scene=''>
-	~~Authors~~: ~~Wang~~, C., ~~Tian~~, T., ~~Zang~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5xoe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XOE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XOE FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure~~ of the ~~apo Staphylococcus aureus~~ phosphofructokinase	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xoe OCA], [http://pdbe.org/5xoe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xoe RCSB], [http://www.ebi.ac.uk/pdbsum/5xoe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xoe ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PFKA_STAAB PFKA_STAAB]] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 6-phosphofructokinase]]
		+	[[Category: Tian, T]]
	[[Category: Wang, C]]		[[Category: Wang, C]]
	[[Category: Zang, J]]		[[Category: Zang, J]]
-	[[Category: ~~Tian, T~~]]	+	[[Category: Phosphofructokinase]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Transferase]]

OCA at 05:17, 14 June 2018

2018-06-14T05:17:56Z

←Older revision		Revision as of 05:17, 14 June 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5xoe is ON HOLD	+	The entry 5xoe is ON HOLD until Jun 26 2019

	Authors: Wang, C., Tian, T., Zang, J.		Authors: Wang, C., Tian, T., Zang, J.
Line 7:		Line 7:
	Description: Crystal Structure of the apo Staphylococcus aureus phosphofructokinase		Description: Crystal Structure of the apo Staphylococcus aureus phosphofructokinase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Tian, T]]
-	[[Category: Zang, J]]
	[[Category: Wang, C]]		[[Category: Wang, C]]
		+	[[Category: Zang, J]]
		+	[[Category: Tian, T]]

OCA: Protected "5xoe" [edit=sysop:move=sysop]

2017-09-27T15:37:36Z

Protected "5xoe" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:37, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xoe is ON HOLD Authors: Wang, C., Tian, T., Zang, J. Description: Crystal Structure of the apo Staphylococcus aureus phosphofructokinase [[Categor...

2017-09-27T15:37:34Z

New page: '''Unreleased structure''' The entry 5xoe is ON HOLD Authors: Wang, C., Tian, T., Zang, J. Description: Crystal Structure of the apo Staphylococcus aureus phosphofructokinase [[Categor...

New page

'''Unreleased structure'''

The entry 5xoe is ON HOLD

Authors: Wang, C., Tian, T., Zang, J.

Description: Crystal Structure of the apo Staphylococcus aureus phosphofructokinase
[[Category: Unreleased Structures]]
[[Category: Tian, T]]
[[Category: Zang, J]]
[[Category: Wang, C]]