5xw2 - Revision history

OCA at 08:15, 22 November 2023

2023-11-22T08:15:59Z

←Older revision		Revision as of 08:15, 22 November 2023
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	<StructureSection load='5xw2' size='340' side='right'caption='[[5xw2]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='5xw2' size='340' side='right'caption='[[5xw2]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xw2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XW2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XW2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_himastatinicus_ATCC_53653 Streptomyces himastatinicus ATCC 53653]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XW2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.298Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e2p\|4e2p]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xw2 OCA], [~~http~~://pdbe.org/5xw2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xw2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xw2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xw2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xw2 OCA], [https://pdbe.org/5xw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xw2 RCSB], [https://www.ebi.ac.uk/pdbsum/5xw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xw2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D9WMQ6_9ACTN D9WMQ6_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Zhang, H D~~]]	+	[[Category: Streptomyces himastatinicus ATCC 53653]]
-	[[Category: Zhang~~, H J~~]]	+	[[Category: Zhang HD]]
-	[[Category: ~~Biosynthetic protein]]~~	+	[[Category: Zhang HJ]]
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Himastatin]]~~	+
-	~~[[Category: Hydroxylase~~]]	+

OCA at 06:59, 14 October 2020

2020-10-14T06:59:51Z

←Older revision		Revision as of 06:59, 14 October 2020
Line 1:		Line 1:

	==Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form==		==Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form==
-	<StructureSection load='5xw2' size='340' side='right' caption='[[5xw2]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='5xw2' size='340' side='right'caption='[[5xw2]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xw2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XW2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XW2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xw2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XW2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XW2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e2p\|4e2p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e2p\|4e2p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xw2 OCA], [http://pdbe.org/5xw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xw2 RCSB], [http://www.ebi.ac.uk/pdbsum/5xw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xw2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xw2 OCA], [http://pdbe.org/5xw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xw2 RCSB], [http://www.ebi.ac.uk/pdbsum/5xw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xw2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5xw2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5xw2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Zhang, H D]]		[[Category: Zhang, H D]]
	[[Category: Zhang, H J]]		[[Category: Zhang, H J]]

OCA at 05:29, 25 April 2018

2018-04-25T05:29:52Z

←Older revision		Revision as of 05:29, 25 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xw2 is ~~ON HOLD~~	+	==Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form==
		+	<StructureSection load='5xw2' size='340' side='right' caption='[[5xw2]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xw2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XW2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e2p\|4e2p]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xw2 OCA], [http://pdbe.org/5xw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xw2 RCSB], [http://www.ebi.ac.uk/pdbsum/5xw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xw2 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Himastatin is a novel antibiotic with antitumor and antibacterial activity. In the himastatin biosynthesis pathway, HmtN is responsible for the hydroxylation of the piperazic acid (Pip) motif. Herein, we present the crystal structures of HmtN (1.3 A), which is the first structure for a cytochrome P450 involved in the hydroxylation of cyclohexadepsipeptide during himastatin biosynthesis. Structure analysis indicated that almost all the surface of HmtN has negative electrostatic potential, only small patches of positive electrostatic potential can be found. It is worth noting that almost the entire active site of HmtT is negatively charged, while HmtN active site is composed of positive and negative charge. This may be relevant to their specific substrate recognition and different catalytic function. In addition, three channels were observed in HmtN crystal structure; channel 3 may be essential for substrate ingress and egress from the active site to the surface, while channel 1 and channel 2 may be the solvent and water pathway, respectively.

-	~~Authors: Zhang, H~~.D., Zhang, H.J.	+	Molecular characterization of the hydroxylase HmtN at 1.3 A resolution.,Zhang H, Chen J, Zhang H Biochem Biophys Res Commun. 2017 Jul 4. pii: S0006-291X(17)31341-4. doi:, 10.1016/j.bbrc.2017.07.010. PMID:28687492<ref>PMID:28687492</ref>

-	~~Description: Crystal structure~~ of the ~~Hydroxylase HmtN in C 1 2 1 crystal form~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Zhang, H.J]]	+	<div class="pdbe-citations 5xw2" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Zhang, H.D~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Zhang, H D]]
		+	[[Category: Zhang, H J]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Cytochrome p450]]
		+	[[Category: Himastatin]]
		+	[[Category: Hydroxylase]]

OCA: Protected "5xw2" [edit=sysop:move=sysop]

2017-09-27T15:50:54Z

Protected "5xw2" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:50, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xw2 is ON HOLD Authors: Zhang, H.D., Zhang, H.J. Description: Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form [[Category: Unrele...

2017-09-27T15:50:52Z

New page: '''Unreleased structure''' The entry 5xw2 is ON HOLD Authors: Zhang, H.D., Zhang, H.J. Description: Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 5xw2 is ON HOLD

Authors: Zhang, H.D., Zhang, H.J.

Description: Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form
[[Category: Unreleased Structures]]
[[Category: Zhang, H.J]]
[[Category: Zhang, H.D]]