5xyt - Revision history

OCA at 10:07, 15 November 2023

2023-11-15T10:07:51Z

←Older revision		Revision as of 10:07, 15 November 2023
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	==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., H130Y muntant==		==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., H130Y muntant==
-	<StructureSection load='5xyt' size='340' side='right'caption='[[5xyt]]' scene=''>	+	<StructureSection load='5xyt' size='340' side='right'caption='[[5xyt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5XYT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacterium_sp._KI723T1 Flavobacterium sp. KI723T1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5xyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyt OCA], [~~http~~://pdbe.org/5xyt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xyt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xyt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyt ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyt OCA], [https://pdbe.org/5xyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyt RCSB], [https://www.ebi.ac.uk/pdbsum/5xyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NYLC_FLASK NYLC_FLASK]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nylon hydrolase (NylC) is initially expressed as an inactive precursor (36 kDa). The precursor is cleaved autocatalytically at Asn266/Thr267 to generate an active enzyme composed of an alpha subunit (27 kDa) and a beta subunit (9 kDa). Four alphabeta heterodimers (molecules A-D) form a doughnut-shaped quaternary structure. In this study, the thermostability of the parental NylC was altered by amino acid substitutions located at the A/D interface (D122G/H130Y/D36A/L137A) or the A/B interface (E263Q) and spanned a range of 47 degrees C. Considering structural, biophysical, and biochemical analyses, we discuss the structural basis of the stability of nylon hydrolase. From the analytical centrifugation data obtained regarding the various mutant enzymes, we conclude that the assembly of the monomeric units is dynamically altered by the mutations. Finally, we propose a model that can predict whether the fate of the nascent polypeptide will be correct subunit assembly, inappropriate protein-protein interactions causing aggregation, or intracellular degradation of the polypeptide.
		+
		+	Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase.,Negoro S, Shibata N, Lee YH, Takehara I, Kinugasa R, Nagai K, Tanaka Y, Kato DI, Takeo M, Goto Y, Higuchi Y Sci Rep. 2018 Jun 27;8(1):9725. doi: 10.1038/s41598-018-27860-w. PMID:29950566<ref>PMID:29950566</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5xyt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Flavobacterium sp. KI723T1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Higuchi Y]]		[[Category: Higuchi Y]]

OCA at 07:01, 14 October 2020

2020-10-14T07:01:39Z

←Older revision		Revision as of 07:01, 14 October 2020
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-	'''Unreleased structure'''

-	~~The entry 5xyt is ON HOLD until Paper Publication~~	+	==Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., H130Y muntant==
-		+	<StructureSection load='5xyt' size='340' side='right'caption='[[5xyt]]' scene=''>
-	~~Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XYT FirstGlance]. <br>
-	~~Description:~~ Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., H130Y muntant	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyt OCA], [http://pdbe.org/5xyt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyt RCSB], [http://www.ebi.ac.uk/pdbsum/5xyt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyt ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Negoro~~, S]]	+	__TOC__
-	[[Category: Higuchi, Y]]	+	</StructureSection>
-	[[Category: Nagai, K]]	+	[[Category: Large Structures]]
-	[[Category: Shibata, N]]	+	[[Category: Higuchi Y]]
		+	[[Category: Nagai K]]
		+	[[Category: Negoro S]]
		+	[[Category: Shibata N]]

OCA: Protected "5xyt" [edit=sysop:move=sysop]

2017-09-27T15:55:41Z

Protected "5xyt" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xyt is ON HOLD until Paper Publication Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y. Description: Structure of 6-aminohexanoate-oligome...

2017-09-27T15:55:40Z

New page: '''Unreleased structure''' The entry 5xyt is ON HOLD until Paper Publication Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y. Description: Structure of 6-aminohexanoate-oligome...

New page

'''Unreleased structure'''

The entry 5xyt is ON HOLD until Paper Publication

Authors: Negoro, S., Shibata, N., Nagai, K., Higuchi, Y.

Description: Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72., H130Y muntant
[[Category: Unreleased Structures]]
[[Category: Negoro, S]]
[[Category: Higuchi, Y]]
[[Category: Nagai, K]]
[[Category: Shibata, N]]