5xyw - Revision history

OCA at 10:20, 27 March 2024

2024-03-27T10:20:58Z

←Older revision		Revision as of 10:20, 27 March 2024
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	==Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain==		==Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain==
-	<StructureSection load='5xyw' size='340' side='right' caption='[[5xyw]], [[Resolution\|resolution]] 2.71Å' scene=''>	+	<StructureSection load='5xyw' size='340' side='right'caption='[[5xyw]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drosi Drosi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5XYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_simulans Drosophila simulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XYW FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~rhi~~, ~~Dsim\GD27086, Dsimw501_GD27086, GD25457 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7240 DROSI~~]~~), Dsim\GD21652, Dsim_GD21652, GD21652 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=7240 DROSI])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.705Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [~~http~~://pdbe.org/5xyw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [https://pdbe.org/5xyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [https://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q49BI5_DROSI Q49BI5_DROSI]
-	PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.	+
-		+
-	~~Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii~~: ~~embr.201745418. doi: 10.15252~~/~~embr.201745418. PMID:29858487<ref>PMID:29858487<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5xyw" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosi~~]]	+	[[Category: Drosophila simulans]]
-	[[Category: ~~Huang, Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Yu, B W~~]]	+	[[Category: Huang Y]]
-	[[Category: ~~Chromoshadow]]~~	+	[[Category: Yu BW]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 09:35, 18 July 2018

2018-07-18T09:35:40Z

←Older revision		Revision as of 09:35, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='5xyw' size='340' side='right' caption='[[5xyw]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='5xyw' size='340' side='right' caption='[[5xyw]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosi Drosi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [http://pdbe.org/5xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [http://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rhi, Dsim\GD27086, Dsimw501_GD27086, GD25457 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7240 DROSI]), Dsim\GD21652, Dsim_GD21652, GD21652 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7240 DROSI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [http://pdbe.org/5xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [http://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drosi]]
	[[Category: Huang, Y]]		[[Category: Huang, Y]]
	[[Category: Yu, B W]]		[[Category: Yu, B W]]

OCA at 05:43, 20 June 2018

2018-06-20T05:43:12Z

←Older revision		Revision as of 05:43, 20 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5xyw is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain==
		+	<StructureSection load='5xyw' size='340' side='right' caption='[[5xyw]], [[Resolution\|resolution]] 2.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5xyw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYW FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyw OCA], [http://pdbe.org/5xyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyw RCSB], [http://www.ebi.ac.uk/pdbsum/5xyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.

-	~~Authors: Yu~~, B~~.W.~~, ~~Huang~~, Y.	+	Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487<ref>PMID:29858487</ref>

-	~~Description: Crystal structure~~ of ~~drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Yu, B.W]]~~	+	<div class="pdbe-citations 5xyw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Huang, Y]]		[[Category: Huang, Y]]
		+	[[Category: Yu, B W]]
		+	[[Category: Chromoshadow]]
		+	[[Category: Complex]]
		+	[[Category: Protein binding]]

OCA: Protected "5xyw" [edit=sysop:move=sysop]

2017-09-27T15:55:49Z

Protected "5xyw" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5xyw is ON HOLD until Paper Publication Authors: Yu, B.W., Huang, Y. Description: Crystal structure of drosophila simulans Rhino chromoshadow domai...

2017-09-27T15:55:48Z

New page: '''Unreleased structure''' The entry 5xyw is ON HOLD until Paper Publication Authors: Yu, B.W., Huang, Y. Description: Crystal structure of drosophila simulans Rhino chromoshadow domai...

New page

'''Unreleased structure'''

The entry 5xyw is ON HOLD until Paper Publication

Authors: Yu, B.W., Huang, Y.

Description: Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain
[[Category: Unreleased Structures]]
[[Category: Yu, B.W]]
[[Category: Huang, Y]]