5y29 - Revision history

OCA at 08:19, 22 November 2023

2023-11-22T08:19:36Z

←Older revision		Revision as of 08:19, 22 November 2023
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	<StructureSection load='5y29' size='340' side='right'caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5y29' size='340' side='right'caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Asian_corn_borer Asian corn borer~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ostrinia_furnacalis Ostrinia furnacalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [~~http~~://pdbe.org/5y29 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [https://pdbe.org/5y29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [https://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A221ZS22_OSTFU A0A221ZS22_OSTFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Asian corn borer]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, W]]	+	[[Category: Ostrinia furnacalis]]
-	[[Category: Qu~~, M B~~]]	+	[[Category: Chen W]]
-	[[Category: Yang, Q]]	+	[[Category: Qu MB]]
-	[[Category: Zhou, Y]]	+	[[Category: Yang Q]]
-	~~[[Category: Gh18 chitinase]]~~	+	[[Category: Zhou Y]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:04, 14 October 2020

2020-10-14T07:04:16Z

←Older revision		Revision as of 07:04, 14 October 2020
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	==Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 1==		==Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 1==
-	<StructureSection load='5y29' size='340' side='right' caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5y29' size='340' side='right'caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asian_corn_borer Asian corn borer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asian_corn_borer Asian corn borer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [http://pdbe.org/5y29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [http://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [http://pdbe.org/5y29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [http://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5y29" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5y29" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Asian corn borer]]		[[Category: Asian corn borer]]
		+	[[Category: Large Structures]]
	[[Category: Chen, W]]		[[Category: Chen, W]]
	[[Category: Qu, M B]]		[[Category: Qu, M B]]

OCA at 20:52, 24 January 2018

2018-01-24T20:52:42Z

←Older revision		Revision as of 20:52, 24 January 2018
Line 3:		Line 3:
	<StructureSection load='5y29' size='340' side='right' caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5y29' size='340' side='right' caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asian_corn_borer Asian corn borer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [http://pdbe.org/5y29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [http://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [http://pdbe.org/5y29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [http://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chitin is a linear homopolymer of N-acetyl-beta-D-glucosamines and a major structural component of insect cuticles. Chitin hydrolysis involves glycoside hydrolase family 18 (GH18) chitinases. In insects, chitin hydrolysis is essential for periodic shedding of the old cuticle ecdysis and proceeds via a pathway different from that in the well-studied bacterial chitinolytic system. Group II chitinase (ChtII) is a widespread chitinolytic enzyme in insects and contains the greatest number of catalytic domains and chitin binding domains among chitinases. In Lepidopterans, ChtII and two other chitinases, ChtI and Chi-h, are essential for chitin hydrolysis. Although ChtI and Chi-h have been well studied, the role of ChtII remains elusive. Here, we investigated the structure and enzymology of OfChtII, a ChtII derived from the insect pest Ostrinia furnacalis We present the crystal structures of two catalytically active domains of OfChtII, OfChtII-C1 and OfChtII-C2, both in unliganded form and complexed with chitooligosaccharide substrates. We found that OfChtII-C1 and OfChtII-C2 both possess long, deep substrate-binding clefts with endochitinase activities. OfChtII exhibited structural characteristics within the substrate-binding cleft similar to those in OfChi-h and OfChtI. However, OfChtII lacked structural elements favoring substrate binding beyond the active sites, including an extra wall structure present in OfChi-h. Nevertheless, the numerous domains in OfChtII may compensate for this difference; a truncation containing one catalytic domain and three chitin binding modules (OfChtII-B4C1) displayed activity toward insoluble polymeric substrates that was higher than those of OfChi-h and OfChtI. Our observations provide the last piece of the puzzle of chitin hydrolysis in insects.
		+
		+	Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects.,Chen W, Qu M, Zhou Y, Yang Q J Biol Chem. 2018 Jan 9. pii: RA117.000119. doi: 10.1074/jbc.RA117.000119. PMID:29317504<ref>PMID:29317504</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5y29" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Asian corn borer]]
	[[Category: Chen, W]]		[[Category: Chen, W]]
	[[Category: Qu, M B]]		[[Category: Qu, M B]]

OCA at 06:57, 17 January 2018

2018-01-17T06:57:47Z

←Older revision		Revision as of 06:57, 17 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5y29 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 1==
-		+	<StructureSection load='5y29' size='340' side='right' caption='[[5y29]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~Authors~~: ~~Chen~~, W., Qu, M.B., ~~Zhou, Y~~., ~~Yang, Q~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5y29]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y29 FirstGlance]. <br>
-	~~Description: Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 1~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~[[Category: Unreleased Structures]]~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y29 OCA], [http://pdbe.org/5y29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y29 RCSB], [http://www.ebi.ac.uk/pdbsum/5y29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y29 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chen, W]]		[[Category: Chen, W]]
		+	[[Category: Qu, M B]]
	[[Category: Yang, Q]]		[[Category: Yang, Q]]
-	[[Category: Qu, M.B]]
	[[Category: Zhou, Y]]		[[Category: Zhou, Y]]
		+	[[Category: Gh18 chitinase]]
		+	[[Category: Hydrolase]]

OCA: Protected "5y29" [edit=sysop:move=sysop]

2017-09-27T16:03:21Z

Protected "5y29" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:03, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5y29 is ON HOLD until Paper Publication Authors: Chen, W., Qu, M.B., Zhou, Y., Yang, Q. Description: Crystal structure of Ostrinia furnacalis Group...

2017-09-27T16:03:20Z

New page: '''Unreleased structure''' The entry 5y29 is ON HOLD until Paper Publication Authors: Chen, W., Qu, M.B., Zhou, Y., Yang, Q. Description: Crystal structure of Ostrinia furnacalis Group...

New page

'''Unreleased structure'''

The entry 5y29 is ON HOLD until Paper Publication

Authors: Chen, W., Qu, M.B., Zhou, Y., Yang, Q.

Description: Crystal structure of Ostrinia furnacalis Group II chitinase catalytic domain 1
[[Category: Unreleased Structures]]
[[Category: Chen, W]]
[[Category: Yang, Q]]
[[Category: Qu, M.B]]
[[Category: Zhou, Y]]