5y4o - Revision history

OCA at 22:40, 10 April 2020

2020-04-10T22:40:47Z

←Older revision		Revision as of 22:40, 10 April 2020
Line 3:		Line 3:
	<SX load='5y4o' size='340' side='right' viewer='molstar' caption='[[5y4o]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='5y4o' size='340' side='right' viewer='molstar' caption='[[5y4o]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y4o]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Y4O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y4o]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y4O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y4o OCA], [http://pdbe.org/5y4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y4o RCSB], [http://www.ebi.ac.uk/pdbsum/5y4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y4o ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y4o OCA], [http://pdbe.org/5y4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y4o RCSB], [http://www.ebi.ac.uk/pdbsum/5y4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y4o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:12, 6 March 2020

2020-03-06T20:12:52Z

←Older revision		Revision as of 20:12, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of MscS channel, YnaI==		==Cryo-EM structure of MscS channel, YnaI==
-	<~~StructureSection~~ load='5y4o' size='340' side='right'caption='[[5y4o]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<SX load='5y4o' size='340' side='right' viewer='molstar' caption='[[5y4o]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5y4o]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y4O FirstGlance]. <br>		<table><tr><td colspan='2'>[[5y4o]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y4O FirstGlance]. <br>
Line 18:		Line 18:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Yu, J]]		[[Category: Yu, J]]

OCA at 07:39, 20 March 2019

2019-03-20T07:39:46Z

←Older revision		Revision as of 07:39, 20 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 5y4o is ~~ON HOLD~~	+	==Cryo-EM structure of MscS channel, YnaI==
		+	<StructureSection load='5y4o' size='340' side='right'caption='[[5y4o]], [[Resolution\|resolution]] 3.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5y4o]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y4O FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y4o OCA], [http://pdbe.org/5y4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y4o RCSB], [http://www.ebi.ac.uk/pdbsum/5y4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y4o ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the YnaI channel as the Na(+)/K(+) cation-selective MS channel and solved its structure at 3.8 A by cryo-EM single-particle method. YnaI exhibits low conductance among the family of MS channels in E. coli, and shares a similar overall heptamer structure fold with previously studied MscS channels. By combining structural based mutagenesis, quantum mechanical and electrophysiological characterizations, we revealed that ion selective filter formed by seven hydrophobic methionine (YnaI(Met158)) in the transmembrane pore determined ion selectivity, and both ion selectivity and gating of YnaI channel were affected by accompanying anions in solution. Further quantum simulation and functional validation support that the distinct binding energies with various anions to YnaI(Met158) facilitate Na(+)/K(+) pass through, which was defined as binding-block mechanism. Our structural and functional studies provided a new perspective for understanding the mechanism of how MS channels select ions driven by mechanical force.

-	~~Authors:~~ Zhang, Y., Yu, J.	+	A binding-block ion selective mechanism revealed by a Na/K selective channel.,Yu J, Zhang B, Zhang Y, Xu CQ, Zhuo W, Ge J, Li J, Gao N, Li Y, Yang M Protein Cell. 2018 Jul;9(7):629-639. doi: 10.1007/s13238-017-0465-8. Epub 2017, Sep 18. PMID:28921397<ref>PMID:28921397</ref>

-	~~Description: Cryo-EM structure of MscS channel~~, ~~YnaI~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Zhang, Y~~]]	+	<div class="pdbe-citations 5y4o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Yu, J]]		[[Category: Yu, J]]
		+	[[Category: Zhang, Y]]
		+	[[Category: Cryo-em]]
		+	[[Category: Membrane protein]]
		+	[[Category: Msc]]
		+	[[Category: Na/k selective channel]]

OCA: Protected "5y4o" [edit=sysop:move=sysop]

2017-09-27T16:08:05Z

Protected "5y4o" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:08, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5y4o is ON HOLD Authors: Zhang, Y., Yu, J. Description: Cryo-EM structure of MscS channel, YnaI Category: Unreleased Structures [[Category: Zha...

2017-09-27T16:08:03Z

New page: '''Unreleased structure''' The entry 5y4o is ON HOLD Authors: Zhang, Y., Yu, J. Description: Cryo-EM structure of MscS channel, YnaI Category: Unreleased Structures [[Category: Zha...

New page

'''Unreleased structure'''

The entry 5y4o is ON HOLD

Authors: Zhang, Y., Yu, J.

Description: Cryo-EM structure of MscS channel, YnaI
[[Category: Unreleased Structures]]
[[Category: Zhang, Y]]
[[Category: Yu, J]]