5yay - Revision history

OCA at 08:25, 22 November 2023

2023-11-22T08:25:34Z

←Older revision		Revision as of 08:25, 22 November 2023
Line 1:		Line 1:

	==Crystal structure of KANK1/KIF21A complex==		==Crystal structure of KANK1/KIF21A complex==
-	<StructureSection load='5yay' size='340' side='right' caption='[[5yay]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='5yay' size='340' side='right'caption='[[5yay]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yay]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YAY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YAY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yay]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YAY FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Kank1 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yay OCA], [~~http~~://pdbe.org/5yay PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yay RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yay PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yay ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yay OCA], [https://pdbe.org/5yay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yay RCSB], [https://www.ebi.ac.uk/pdbsum/5yay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yay ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~KI21A_MOUSE KI21A_MOUSE~~]~~] Microtubule-binding motor protein probably involved in neuronal axonal transport. In vitro, has a plus-end directed motor activity.<ref>PMID:10225949</ref>~~	+	[https://www.uniprot.org/uniprot/E9Q238_MOUSE E9Q238_MOUSE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pan, W~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Wei, Z~~]]	+	[[Category: Pan W]]
-	[[Category: ~~Ank repeat]]~~	+	[[Category: Wei Z]]
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Scaffold protein~~]]	+

OCA at 08:58, 27 December 2017

2017-12-27T08:58:31Z

←Older revision		Revision as of 08:58, 27 December 2017
Line 3:		Line 3:
	<StructureSection load='5yay' size='340' side='right' caption='[[5yay]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='5yay' size='340' side='right' caption='[[5yay]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yay]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YAY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yay]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YAY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yay OCA], [http://pdbe.org/5yay PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yay RCSB], [http://www.ebi.ac.uk/pdbsum/5yay PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yay ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kank1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yay OCA], [http://pdbe.org/5yay PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yay RCSB], [http://www.ebi.ac.uk/pdbsum/5yay PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yay ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KI21A_MOUSE KI21A_MOUSE]] Microtubule-binding motor protein probably involved in neuronal axonal transport. In vitro, has a plus-end directed motor activity.<ref>PMID:10225949</ref>		[[http://www.uniprot.org/uniprot/KI21A_MOUSE KI21A_MOUSE]] Microtubule-binding motor protein probably involved in neuronal axonal transport. In vitro, has a plus-end directed motor activity.<ref>PMID:10225949</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kidney ankyrin repeat-containing proteins (KANK1/2/3/4) belong to a family of scaffold proteins, playing critical roles in cytoskeleton organization, cell polarity and migration. Mutations in KANK proteins are implicated in cancers and genetic diseases, such as nephrotic syndrome. KANK proteins can bind various target proteins through different protein regions, including a highly conserved ankyrin repeat domain (ANKRD). However, the molecular basis for target recognition by the ANKRD remains elusive. In this study, we solved a high-resolution crystal structure of the ANKRD of KANK1 in complex with a short sequence of the motor protein kinesin family member 21A (KIF21A), revealing that the highly specific target-binding mode of the ANKRD involves combinatorial use of two interfaces. Mutations in either interface disrupted the KANK1/KIF21A interaction. Cellular immunofluorescence localization analysis indicates that binding-deficient mutations block recruitment of KIF21A to focal adhesions by KANK1. In conclusion, our structural study provides mechanistic explanations for the ANKRD-mediated recognition of KIF21A and for many disease-related mutations identified in human KANK proteins.
		+
		+	Structural insights into ankyrin repeat-mediated recognition of the kinesin motor protein KIF21A by KANK1, a scaffold protein in focal adhesion.,Pan W, Sun K, Tang K, Xiao Q, Ma C, Yu C, Wei Z J Biol Chem. 2017 Dec 7. pii: M117.815779. doi: 10.1074/jbc.M117.815779. PMID:29217769<ref>PMID:29217769</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5yay" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Pan, W]]		[[Category: Pan, W]]
	[[Category: Wei, Z]]		[[Category: Wei, Z]]

OCA at 06:22, 20 December 2017

2017-12-20T06:22:29Z

←Older revision		Revision as of 06:22, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5yay is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Crystal structure of KANK1/KIF21A complex==
-		+	<StructureSection load='5yay' size='340' side='right' caption='[[5yay]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5yay]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YAY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yay OCA], [http://pdbe.org/5yay PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yay RCSB], [http://www.ebi.ac.uk/pdbsum/5yay PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yay ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KI21A_MOUSE KI21A_MOUSE]] Microtubule-binding motor protein probably involved in neuronal axonal transport. In vitro, has a plus-end directed motor activity.<ref>PMID:10225949</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pan, W]]
		+	[[Category: Wei, Z]]
		+	[[Category: Ank repeat]]
		+	[[Category: Protein binding]]
		+	[[Category: Scaffold protein]]

OCA: Protected "5yay" [edit=sysop:move=sysop]

2017-09-27T16:21:16Z

Protected "5yay" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:21, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5yay is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2017-09-27T16:21:15Z

New page: '''Unreleased structure''' The entry 5yay is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5yay is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]