5yuq - Revision history

OCA at 08:38, 22 November 2023

2023-11-22T08:38:19Z

←Older revision		Revision as of 08:38, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5yuq' size='340' side='right'caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='5yuq' size='340' side='right'caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_16457 Atcc 16457~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[5xwq\|5xwq]]~~, [[~~5xwf~~\|~~5xwf~~]]~~</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.56Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.~~14 3.2.1.14] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [https://pdbe.org/5yuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [https://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [https://pdbe.org/5yuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [https://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A3B6UEQ2_RHIMI A0A3B6UEQ2_RHIMI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 16457]]
-	[[Category: Chitinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hu, S Q]]
-	[[Category: Jiang, Z Q]]
-	[[Category: Liu, Y C]]
-	[[Category: Qin, Z]]
-	[[Category: Yan, Q J]]
-	[[Category: Yang, S Q]]
-	[[Category: Hydrolase]]
	[[Category: Rhizomucor miehei]]		[[Category: Rhizomucor miehei]]
		+	[[Category: Hu SQ]]
		+	[[Category: Jiang ZQ]]
		+	[[Category: Liu YC]]
		+	[[Category: Qin Z]]
		+	[[Category: Yan QJ]]
		+	[[Category: Yang SQ]]

OCA at 06:12, 18 August 2021

2021-08-18T06:12:49Z

←Older revision		Revision as of 06:12, 18 August 2021
Line 3:		Line 3:
	<StructureSection load='5yuq' size='340' side='right'caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='5yuq' size='340' side='right'caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xwq\|5xwq]], [[5xwf\|5xwf]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5xwq\|5xwq]], [[5xwf\|5xwf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [~~http~~://pdbe.org/5yuq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [https://pdbe.org/5yuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [https://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A chitinase gene (RmChiA) encoding 445 amino acid (aa) residues from a fungus Rhizomucor miehei was cloned and overexpressed in Escherichia coli. Two kinds of RmChiA crystal forms, with space groups P32 2 1 and P1, were obtained by sitting-drop vapor diffusion and the structures were determined by X-ray diffraction. The overall structure of RmChiA monomer, which is the first structure of bacterial-type chitinases from nonpathogenic fungi, adopts a canonical triosephosphate isomerase (TIM) barrel fold with two protruding chitinase insertion domains. RmChiA exhibited a unique NxDxE catalytical motif and a real active site tunnel structure, which are firstly found in GH family 18 chitinases. The motif had high structural homolog with the typical DxDxE motif in other GH family 18 chitinases. The tunnel is formed by two unusual long loops, containing 15 aa and 45 aa respectively, linked by a disulfide bond across the substrate-binding cleft. Mutation experiments found that opening the roof of tunnel structure increased the hydrolysis efficiency of RmChiA, but the thermostability of the mutants decreased. Moreover, the tunnel structure endowed RmChiA with the exo-chitinase character.
		+
		+	Crystal structure of a chitinase (RmChiA) from the thermophilic fungus Rhizomucor miehei with a real active site tunnel.,Jiang Z, Hu S, Ma J, Liu Y, Qiao Z, Yan Q, Gao Y, Yang S Biochim Biophys Acta Proteins Proteom. 2021 Aug 4;1869(11):140709. doi:, 10.1016/j.bbapap.2021.140709. PMID:34358705<ref>PMID:34358705</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5yuq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Chitinase 3D structures\|Chitinase 3D structures]]		*[[Chitinase 3D structures\|Chitinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:28, 21 October 2020

2020-10-21T09:28:30Z

←Older revision		Revision as of 09:28, 21 October 2020
Line 1:		Line 1:

	==The high resolution structure of chitinase (RmChi1) from the thermophilic fungus Rhizomucor miehei (sp P1)==		==The high resolution structure of chitinase (RmChi1) from the thermophilic fungus Rhizomucor miehei (sp P1)==
-	<StructureSection load='5yuq' size='340' side='right' caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>	+	<StructureSection load='5yuq' size='340' side='right'caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xwq\|5xwq]], [[5xwf\|5xwf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xwq\|5xwq]], [[5xwf\|5xwf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [http://pdbe.org/5yuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [http://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [http://pdbe.org/5yuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [http://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Atcc 16457]]		[[Category: Atcc 16457]]
	[[Category: Chitinase]]		[[Category: Chitinase]]
		+	[[Category: Large Structures]]
	[[Category: Hu, S Q]]		[[Category: Hu, S Q]]
	[[Category: Jiang, Z Q]]		[[Category: Jiang, Z Q]]

OCA at 20:22, 2 December 2018

2018-12-02T20:22:22Z

←Older revision		Revision as of 20:22, 2 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5yuq ~~is ON HOLD until Nov 23 2019~~	+	==The high resolution structure of chitinase (RmChi1) from the thermophilic fungus Rhizomucor miehei (sp P1)==
-		+	<StructureSection load='5yuq' size='340' side='right' caption='[[5yuq]], [[Resolution\|resolution]] 1.56Å' scene=''>
-	~~Authors~~: ~~Jiang~~, Z.Q., ~~Hu, S~~.Q., ~~Liu, Y~~.C., ~~Qin~~, Z., ~~Yan, Q~~.J., ~~Yang, S~~.Q.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5yuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YUQ FirstGlance]. <br>
-	~~Description: The high resolution structure of chitinase (RmChi1) from the thermophilic fungus Rhizomucor miehei (sp P1)~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5xwq\|5xwq]], [[5xwf\|5xwf]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
-	[[Category: ~~Jiang, Z.Q~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yuq OCA], [http://pdbe.org/5yuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yuq RCSB], [http://www.ebi.ac.uk/pdbsum/5yuq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yuq ProSAT]</span></td></tr>
-	[[Category: ~~Liu~~, ~~Y.C~~]]	+	</table>
-	[[Category: ~~Yan~~, Q.J]]	+	__TOC__
-	[[Category: Hu, ~~S.Q~~]]	+	</StructureSection>
		+	[[Category: Atcc 16457]]
		+	[[Category: Chitinase]]
		+	[[Category: Hu, S Q]]
		+	[[Category: Jiang, Z Q]]
		+	[[Category: Liu, Y C]]
	[[Category: Qin, Z]]		[[Category: Qin, Z]]
-	[[Category: Yang, S.Q]]	+	[[Category: Yan, Q J]]
		+	[[Category: Yang, S Q]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rhizomucor miehei]]

OCA at 04:49, 3 January 2018

2018-01-03T04:49:38Z

←Older revision		Revision as of 04:49, 3 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5yuq is ON HOLD	+	The entry 5yuq is ON HOLD until Nov 23 2019

	Authors: Jiang, Z.Q., Hu, S.Q., Liu, Y.C., Qin, Z., Yan, Q.J., Yang, S.Q.		Authors: Jiang, Z.Q., Hu, S.Q., Liu, Y.C., Qin, Z., Yan, Q.J., Yang, S.Q.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Jiang, Z.Q]]		[[Category: Jiang, Z.Q]]
-	[[Category: Qin, Z]]
-	[[Category: Yang, S.Q]]
	[[Category: Liu, Y.C]]		[[Category: Liu, Y.C]]
	[[Category: Yan, Q.J]]		[[Category: Yan, Q.J]]
	[[Category: Hu, S.Q]]		[[Category: Hu, S.Q]]
		+	[[Category: Qin, Z]]
		+	[[Category: Yang, S.Q]]

OCA: Protected "5yuq" [edit=sysop:move=sysop]

2017-11-29T05:00:30Z

Protected "5yuq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 29 November 2017

OCA: New page: '''Unreleased structure''' The entry 5yuq is ON HOLD Authors: Jiang, Z.Q., Hu, S.Q., Liu, Y.C., Qin, Z., Yan, Q.J., Yang, S.Q. Description: The high resolution structure of chitinase (...

2017-11-29T05:00:29Z

New page: '''Unreleased structure''' The entry 5yuq is ON HOLD Authors: Jiang, Z.Q., Hu, S.Q., Liu, Y.C., Qin, Z., Yan, Q.J., Yang, S.Q. Description: The high resolution structure of chitinase (...

New page

'''Unreleased structure'''

The entry 5yuq is ON HOLD

Authors: Jiang, Z.Q., Hu, S.Q., Liu, Y.C., Qin, Z., Yan, Q.J., Yang, S.Q.

Description: The high resolution structure of chitinase (RmChi1) from the thermophilic fungus Rhizomucor miehei (sp P1)
[[Category: Unreleased Structures]]
[[Category: Jiang, Z.Q]]
[[Category: Qin, Z]]
[[Category: Yang, S.Q]]
[[Category: Liu, Y.C]]
[[Category: Yan, Q.J]]
[[Category: Hu, S.Q]]