5z65 - Revision history

OCA at 08:46, 22 November 2023

2023-11-22T08:46:10Z

←Older revision		Revision as of 08:46, 22 November 2023
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	<StructureSection load='5z65' size='340' side='right'caption='[[5z65]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5z65' size='340' side='right'caption='[[5z65]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z65]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pig Pig~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z65 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5Z65 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z65]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z65 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ANPEP, pAPN ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id~~=~~9823 PIG])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z65 OCA], [~~http~~://pdbe.org/5z65 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z65 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z65 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z65 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z65 OCA], [https://pdbe.org/5z65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z65 RCSB], [https://www.ebi.ac.uk/pdbsum/5z65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z65 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AMPN_PIG AMPN_PIG] Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis (By similarity). It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide. In case of porcine transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCoV) infections, serves as a receptor for TGEV and PRCoV spike glycoprotein in a species-specific manner.<ref>PMID:7913510</ref> <ref>PMID:1350661</ref> <ref>PMID:7911642</ref> <ref>PMID:8985407</ref> <ref>PMID:9634079</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pig~~]]	+	[[Category: Sus scrofa]]
-	[[Category: Jiang~~, L G~~]]	+	[[Category: Jiang LG]]
-	[[Category: Li, R]]	+	[[Category: Li R]]
-	[[Category: Qiao~~, S L~~]]	+	[[Category: Qiao SL]]
-	[[Category: Sun~~, Y G~~]]	+	[[Category: Sun YG]]
-	[[Category: Zhang~~, G P]]~~	+	[[Category: Zhang GP]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 21:14, 28 October 2020

2020-10-28T21:14:57Z

←Older revision		Revision as of 21:14, 28 October 2020
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	==Crystal structure of porcine aminopeptidase N ectodomain in functional form==		==Crystal structure of porcine aminopeptidase N ectodomain in functional form==
-	<StructureSection load='5z65' size='340' side='right' caption='[[5z65]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5z65' size='340' side='right'caption='[[5z65]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z65]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z65 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z65 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z65]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z65 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z65 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z65 OCA], [http://pdbe.org/5z65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z65 RCSB], [http://www.ebi.ac.uk/pdbsum/5z65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z65 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANPEP, pAPN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z65 OCA], [http://pdbe.org/5z65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z65 RCSB], [http://www.ebi.ac.uk/pdbsum/5z65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z65 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5z65" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5z65" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aminopeptidase 3D structures\|Aminopeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pig]]
	[[Category: Jiang, L G]]		[[Category: Jiang, L G]]
	[[Category: Li, R]]		[[Category: Li, R]]

OCA at 06:07, 2 January 2019

2019-01-02T06:07:23Z

←Older revision		Revision as of 06:07, 2 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 5z65 is ~~ON HOLD~~	+	==Crystal structure of porcine aminopeptidase N ectodomain in functional form==
		+	<StructureSection load='5z65' size='340' side='right' caption='[[5z65]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z65]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z65 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z65 OCA], [http://pdbe.org/5z65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z65 RCSB], [http://www.ebi.ac.uk/pdbsum/5z65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z65 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent studies have indicated that pAPN is not a functional receptor for PEDV. To date, there is a lack of a direct evidence for the interaction between pAPN and PEDV S protein in vitro. Here, we prepared pAPN ectodomain and the truncated variants of PEDV S protein in Drosophila S2 cells. These recombinant proteins were homogeneous after purification by metal-affinity and size-exclusion chromatography. We then assayed the purified target proteins through immunogenicity tests, PEDV binding interference assays, circular dichroism (CD) measurements, pAPN activity assay and structural determination, demonstrating that they were biologically functional. Finally, we characterized their interactions by gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and surface plasmon resonance (SPR) analyses. The results showed that their affinities were too low to form complexes, which suggest that pAPN may be controversial as the genuine receptor for PEDV. Therefore, further research needs to be carried out to elucidate the interaction between PEDV and its genuine receptor.

-	~~Authors: Sun, Y.G~~., Li, R., Jiang, L~~.G.~~, Qiao, S~~.L.~~, ~~Zhang~~, G.P.	+	Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus.,Sun YG, Li R, Jiang L, Qiao S, Zhi Y, Chen XX, Xie S, Wu J, Li X, Deng R, Zhang G Int J Biol Macromol. 2018 Oct 1;117:704-712. doi: 10.1016/j.ijbiomac.2018.05.167., Epub 2018 May 24. PMID:29802920<ref>PMID:29802920</ref>

-	~~Description: Crystal structure~~ of ~~porcine aminopeptidase N ectodomain in functional form~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Qiao~~, S.L]]	+	<div class="pdbe-citations 5z65" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Jiang, L G]]
	[[Category: Li, R]]		[[Category: Li, R]]
-	[[Category: ~~Zhang~~, ~~G.P~~]]	+	[[Category: Qiao, S L]]
-	[[Category: ~~Jiang~~, L.G]]	+	[[Category: Sun, Y G]]
-	[[Category: ~~Sun~~, Y.G]]	+	[[Category: Zhang, G P]]
		+	[[Category: Hydrolase]]

OCA: Protected "5z65" [edit=sysop:move=sysop]

2018-01-31T04:40:21Z

Protected "5z65" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z65 is ON HOLD Authors: Sun, Y.G., Li, R., Jiang, L.G., Qiao, S.L., Zhang, G.P. Description: Crystal structure of porcine aminopeptidase N ectodom...

2018-01-31T04:40:20Z

New page: '''Unreleased structure''' The entry 5z65 is ON HOLD Authors: Sun, Y.G., Li, R., Jiang, L.G., Qiao, S.L., Zhang, G.P. Description: Crystal structure of porcine aminopeptidase N ectodom...

New page

'''Unreleased structure'''

The entry 5z65 is ON HOLD

Authors: Sun, Y.G., Li, R., Jiang, L.G., Qiao, S.L., Zhang, G.P.

Description: Crystal structure of porcine aminopeptidase N ectodomain in functional form
[[Category: Unreleased Structures]]
[[Category: Qiao, S.L]]
[[Category: Li, R]]
[[Category: Zhang, G.P]]
[[Category: Jiang, L.G]]
[[Category: Sun, Y.G]]