5zbe - Revision history

OCA at 08:51, 22 November 2023

2023-11-22T08:51:06Z

←Older revision		Revision as of 08:51, 22 November 2023
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	<StructureSection load='5zbe' size='340' side='right'caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5zbe' size='340' side='right'caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Microcystis_aeruginosa_dianchi905~~ Microcystis aeruginosa ~~dianchi905~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_FACHB-905_=_DIANCHI905 Microcystis aeruginosa FACHB-905 = DIANCHI905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FE2:FE+(II)+ION'>FE2</scene~~>, ~~<scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~aerE, C789_497 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode=5&id~~=~~1235808 Microcystis aeruginosa DIANCHI905])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zbe OCA], [~~http~~://pdbe.org/5zbe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zbe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zbe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zbe ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zbe OCA], [https://pdbe.org/5zbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zbe RCSB], [https://www.ebi.ac.uk/pdbsum/5zbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zbe ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Microcystis aeruginosa ~~dianchi905~~]]	+	[[Category: Microcystis aeruginosa FACHB-905 = DIANCHI905]]
-	[[Category: Qiu, X]]	+	[[Category: Qiu X]]
-	~~[[Category: Choi moiety of aeruginosin]]~~	+
-	~~[[Category: Cupin superfamily enzyme]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 06:56, 27 March 2019

2019-03-27T06:56:07Z

←Older revision		Revision as of 06:56, 27 March 2019
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	==Crystal structure of AerE from Microcystis aeruginosa==		==Crystal structure of AerE from Microcystis aeruginosa==
-	<StructureSection load='5zbe' size='340' side='right' caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5zbe' size='340' side='right'caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa_dianchi905 Microcystis aeruginosa dianchi905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa_dianchi905 Microcystis aeruginosa dianchi905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Microcystis aeruginosa dianchi905]]		[[Category: Microcystis aeruginosa dianchi905]]
	[[Category: Qiu, X]]		[[Category: Qiu, X]]

OCA at 15:52, 27 February 2019

2019-02-27T15:52:57Z

←Older revision		Revision as of 15:52, 27 February 2019
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	<StructureSection load='5zbe' size='340' side='right' caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5zbe' size='340' side='right' caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa_dianchi905 Microcystis aeruginosa dianchi905]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aerE, C789_497 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1235808 Microcystis aeruginosa DIANCHI905])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zbe OCA], [http://pdbe.org/5zbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zbe RCSB], [http://www.ebi.ac.uk/pdbsum/5zbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zbe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zbe OCA], [http://pdbe.org/5zbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zbe RCSB], [http://www.ebi.ac.uk/pdbsum/5zbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zbe ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 2-carboxy-6-hydroxyoctahydroindole (Choi) moiety is a hallmark of aeruginosins, a class of cyanobacterial derived bioactive linear tetrapeptides that possess antithrombotic activity. The biosynthetic pathway of Choi has yet to be resolved. AerE is a cupin superfamily enzyme that was shown to be involved in the biosynthesis of Choi, but its exact role remains unclear. This study reports the functional characterization and structural analyses of AerE. Enzymatic observation reveals that AerE can dramatically accelerate 1,3-allylic isomerization of the non-aromatic decarboxylation product of prephenate, dihydro-4-hydroxyphenylpyruvate (H2HPP). This olefin isomerization reaction can occur non-enzymatically and is the second step of the biosynthetic pathway from prephenate to Choi. The results of comparative structural analysis and substrate analogue binding geometry analysis combined with the results of mutational studies suggest that AerE employs an induced fit strategy to bind and stabilize the substrate in a particular conformation that is possibly favorable for 1,3-allylic isomerization of H2HPP through coordinate bonds, hydrogen bonds, pi-pi conjugation interaction and hydrophobic interactions. All of these interactions are critical for the catalytic efficiency.
		+
		+	Structural and functional insights into the role of a cupin superfamily isomerase in the biosynthesis of Choi moiety of aeruginosin.,Qiu X, Zhu W, Wang W, Jin H, Zhu P, Zhuang R, Yan X J Struct Biol. 2019 Feb 10. pii: S1047-8477(19)30012-7. doi:, 10.1016/j.jsb.2019.01.007. PMID:30742895<ref>PMID:30742895</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5zbe" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Microcystis aeruginosa dianchi905]]
	[[Category: Qiu, X]]		[[Category: Qiu, X]]
	[[Category: Choi moiety of aeruginosin]]		[[Category: Choi moiety of aeruginosin]]
	[[Category: Cupin superfamily enzyme]]		[[Category: Cupin superfamily enzyme]]
	[[Category: Isomerase]]		[[Category: Isomerase]]

OCA at 09:24, 13 February 2019

2019-02-13T09:24:34Z

←Older revision		Revision as of 09:24, 13 February 2019
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-	'''Unreleased structure'''

-	~~The entry 5zbe is ON HOLD until Paper Publication~~	+	==Crystal structure of AerE from Microcystis aeruginosa==
-		+	<StructureSection load='5zbe' size='340' side='right' caption='[[5zbe]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~Authors: Qiu, X.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5zbe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZBE FirstGlance]. <br>
-	~~Description:~~ Crystal structure of AerE from Microcystis aeruginosa	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zbe OCA], [http://pdbe.org/5zbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zbe RCSB], [http://www.ebi.ac.uk/pdbsum/5zbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zbe ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Qiu, X]]		[[Category: Qiu, X]]
		+	[[Category: Choi moiety of aeruginosin]]
		+	[[Category: Cupin superfamily enzyme]]
		+	[[Category: Isomerase]]

OCA at 12:15, 11 April 2018

2018-04-11T12:15:40Z

←Older revision		Revision as of 12:15, 11 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zbe is ON HOLD	+	The entry 5zbe is ON HOLD until Paper Publication

	Authors: Qiu, X.		Authors: Qiu, X.

OCA at 04:28, 4 April 2018

2018-04-04T04:28:39Z

←Older revision		Revision as of 04:28, 4 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zbe is ON HOLD ~~until Paper Publication~~	+	The entry 5zbe is ON HOLD

	Authors: Qiu, X.		Authors: Qiu, X.

OCA at 05:07, 28 March 2018

2018-03-28T05:07:29Z

←Older revision		Revision as of 05:07, 28 March 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zbe is ON HOLD	+	The entry 5zbe is ON HOLD until Paper Publication

	Authors: Qiu, X.		Authors: Qiu, X.

OCA at 05:56, 21 March 2018

2018-03-21T05:56:20Z

←Older revision		Revision as of 05:56, 21 March 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zbe is ON HOLD ~~until Paper Publication~~	+	The entry 5zbe is ON HOLD

	Authors: Qiu, X.		Authors: Qiu, X.

OCA: Protected "5zbe" [edit=sysop:move=sysop]

2018-02-28T04:53:00Z

Protected "5zbe" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:53, 28 February 2018

OCA: New page: '''Unreleased structure''' The entry 5zbe is ON HOLD until Paper Publication Authors: Qiu, X. Description: Crystal structure of AerE from Microcystis aeruginosa [[Category: Unreleased ...

2018-02-28T04:52:59Z

New page: '''Unreleased structure''' The entry 5zbe is ON HOLD until Paper Publication Authors: Qiu, X. Description: Crystal structure of AerE from Microcystis aeruginosa [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 5zbe is ON HOLD until Paper Publication

Authors: Qiu, X.

Description: Crystal structure of AerE from Microcystis aeruginosa
[[Category: Unreleased Structures]]
[[Category: Qiu, X]]