5zcx - Revision history

OCA at 21:18, 28 October 2020

2020-10-28T21:18:39Z

←Older revision		Revision as of 21:18, 28 October 2020
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	==Structure of T20/N39==		==Structure of T20/N39==
-	<StructureSection load='5zcx' size='340' side='right' caption='[[5zcx]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5zcx' size='340' side='right'caption='[[5zcx]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zcx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZCX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zcx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZCX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcx OCA], [http://pdbe.org/5zcx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zcx RCSB], [http://www.ebi.ac.uk/pdbsum/5zcx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcx OCA], [http://pdbe.org/5zcx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zcx RCSB], [http://www.ebi.ac.uk/pdbsum/5zcx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ding, X H]]		[[Category: Ding, X H]]
	[[Category: Zhang, X J]]		[[Category: Zhang, X J]]

OCA at 08:00, 10 October 2018

2018-10-10T08:00:14Z

←Older revision		Revision as of 08:00, 10 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5zcx is ~~ON HOLD~~	+	==Structure of T20/N39==
		+	<StructureSection load='5zcx' size='340' side='right' caption='[[5zcx]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zcx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZCX FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcx OCA], [http://pdbe.org/5zcx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zcx RCSB], [http://www.ebi.ac.uk/pdbsum/5zcx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	OBJECTIVE: The peptide drug T20 (enfuvirtide), derived from the C-terminal heptad repeat region of HIV-1 gp41, is the only membrane fusion inhibitor available for treatment of viral infection; however, its mechanism of action remains elusive and its structural basis is lacking. DESIGN: We focused on determining the crystal structure of T20 in complex with N39, a target mimic peptide derived from the N-terminal heptad repeat region of gp41. Based on the structural information, the mechanisms of action of T20 and its resistance were further characterized. METHODS: A panel of peptides was synthesized. The T20/N39 complex was assembled for crystallization studies. Circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), native polyacrylamide gel electrophoresis (N-PAGE), and mutational analysis were applied to analyze the structural and functional properties. RESULTS: A crystal structure of six-helical bundle (6-HB) structure formed by T20 and N39 was determined with a resolution limit of 2.3 A, which revealed the critical intra- and inter-helical interactions underlying the mechanism of action of T20 and its resistance mutations. While the structural properties in the C-terminal tryptophan-rich motif (TRM) of T20 and the fusion peptide proximal region (FPPR) of N39 could not be finely defined by the structure, the data from biophysical and mutational analyses verified the essential roles of the TRM and FPPR motifs for the binding and inhibitory activities of T20. CONCLUSION: For the first time, our studies provide a structural basis of T20, which help our understanding on the mechanisms of HIV-1 fusion and its inhibition.

-	~~Authors: Zhang~~, X~~.J.~~, Ding, X.H.	+	Structural and functional characterization of HIV-1 cell fusion inhibitor T20.,Zhang X, Ding X, Zhu Y, Chong H, Cui S, He J, Wang X, He Y AIDS. 2018 Aug 8. doi: 10.1097/QAD.0000000000001979. PMID:30096076<ref>PMID:30096076</ref>

-	~~Description~~: ~~T20~~/~~N39~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Zhang, X.J]]	+	<div class="pdbe-citations 5zcx" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ding, X.H~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ding, X H]]
		+	[[Category: Zhang, X J]]
		+	[[Category: 6-hb]]
		+	[[Category: Helice]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "5zcx" [edit=sysop:move=sysop]

2018-02-28T04:54:51Z

Protected "5zcx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 28 February 2018

OCA: New page: '''Unreleased structure''' The entry 5zcx is ON HOLD Authors: Zhang, X.J., Ding, X.H. Description: T20/N39 Category: Unreleased Structures Category: Zhang, X.J [[Category: Ding...

2018-02-28T04:54:50Z

New page: '''Unreleased structure''' The entry 5zcx is ON HOLD Authors: Zhang, X.J., Ding, X.H. Description: T20/N39 Category: Unreleased Structures Category: Zhang, X.J [[Category: Ding...

New page

'''Unreleased structure'''

The entry 5zcx is ON HOLD

Authors: Zhang, X.J., Ding, X.H.

Description: T20/N39
[[Category: Unreleased Structures]]
[[Category: Zhang, X.J]]
[[Category: Ding, X.H]]