5zhy - Revision history

OCA at 08:56, 22 November 2023

2023-11-22T08:56:12Z

←Older revision		Revision as of 08:56, 22 November 2023
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	<StructureSection load='5zhy' size='340' side='right'caption='[[5zhy]], [[Resolution\|resolution]] 2.44Å' scene=''>		<StructureSection load='5zhy' size='340' side='right'caption='[[5zhy]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zhy]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zhy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_229E Human coronavirus 229E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhy OCA], [~~http~~://pdbe.org/5zhy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zhy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zhy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.441Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhy OCA], [https://pdbe.org/5zhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zhy RCSB], [https://www.ebi.ac.uk/pdbsum/5zhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SPIKE_CVH22 SPIKE_CVH22]] S1 region attaches the virion to the cell membrane by interacting with human ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).	+	[https://www.uniprot.org/uniprot/SPIKE_CVH22 SPIKE_CVH22] S1 region attaches the virion to the cell membrane by interacting with human ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human coronavirus 229E]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, X]]	+	[[Category: Chen X]]
-	[[Category: Rao, Z]]	+	[[Category: Rao Z]]
-	[[Category: Yan, M]]	+	[[Category: Yan M]]
-	[[Category: Yang, H]]	+	[[Category: Yang H]]
-	[[Category: Zhang, W]]	+	[[Category: Zhang W]]
-	[[Category: Zheng, Q]]	+	[[Category: Zheng Q]]
-	[[Category: Zhou, W]]	+	[[Category: Zhou W]]
-	~~[[Category: Broad-spectrum inhibitor design]]~~	+
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 21:19, 28 October 2020

2020-10-28T21:19:51Z

←Older revision		Revision as of 21:19, 28 October 2020
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	==Structural characterization of the HCoV-229E fusion core==		==Structural characterization of the HCoV-229E fusion core==
-	<StructureSection load='5zhy' size='340' side='right' caption='[[5zhy]], [[Resolution\|resolution]] 2.44Å' scene=''>	+	<StructureSection load='5zhy' size='340' side='right'caption='[[5zhy]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zhy]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zhy]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZHY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhy OCA], [http://pdbe.org/5zhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zhy RCSB], [http://www.ebi.ac.uk/pdbsum/5zhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhy ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhy OCA], [http://pdbe.org/5zhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zhy RCSB], [http://www.ebi.ac.uk/pdbsum/5zhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5zhy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5zhy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sandbox 3001\|Sandbox 3001]]
		+	*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chen, X]]		[[Category: Chen, X]]
	[[Category: Rao, Z]]		[[Category: Rao, Z]]

OCA at 05:54, 6 June 2018

2018-06-06T05:54:03Z

←Older revision		Revision as of 05:54, 6 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zhy is ~~ON HOLD~~	+	==Structural characterization of the HCoV-229E fusion core==
		+	<StructureSection load='5zhy' size='340' side='right' caption='[[5zhy]], [[Resolution\|resolution]] 2.44Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zhy]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhy OCA], [http://pdbe.org/5zhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zhy RCSB], [http://www.ebi.ac.uk/pdbsum/5zhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhy ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPIKE_CVH22 SPIKE_CVH22]] S1 region attaches the virion to the cell membrane by interacting with human ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HCoV-229E spike (S) protein mediates virion attachment to cells and subsequent fusion of the viral and cellular membranes. This protein is composed of an N-terminal receptor-binding domain (S1) and a C-terminal trans-membrane fusion domain (S2). S2 contains a highly conserved heptad repeat 1 and 2 (HR1 and HR2). In this study, the HRs sequences were designed and connected with a flexible linker. The recombinant fusion core protein was crystallized and its structure was solved at a resolution of 2.45A. Then we characterized the binding of HR1s and HR2s via both sequence alignment and structural analysis. The overall structures, especially the residues in some positions of HR2 are highly conserved. Fourteen hydrophobic and three polar residues from each HR1 peptide are packed in layers at the coiled-coil interface. These core amino acids can be grouped into seven heptad repeats. Analysis of hydrophobic and hydrophilic interactions between HR2 helix and HR1 helices, shows that the HR1 and HR2 polypeptides are highly complementary in both shape and chemical properties. Furthermore, the available knowledge concerning HCoV-229E fusion core may make it possible to design small molecule or polypeptide drugs targeting membrane fusion, a crucial step of HCoV-229E infection.

-	~~Authors~~:	+	Structural characterization of the HCoV-229E fusion core.,Zhang W, Zheng Q, Yan M, Chen X, Yang H, Zhou W, Rao Z Biochem Biophys Res Commun. 2018 Mar 4;497(2):705-712. doi:, 10.1016/j.bbrc.2018.02.136. Epub 2018 Feb 16. PMID:29458023<ref>PMID:29458023</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5zhy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, X]]
		+	[[Category: Rao, Z]]
		+	[[Category: Yan, M]]
		+	[[Category: Yang, H]]
		+	[[Category: Zhang, W]]
		+	[[Category: Zheng, Q]]
		+	[[Category: Zhou, W]]
		+	[[Category: Broad-spectrum inhibitor design]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Viral protein]]

OCA: Protected "5zhy" [edit=sysop:move=sysop]

2018-03-21T06:01:49Z

Protected "5zhy" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:01, 21 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zhy is ON HOLD Authors: Description: Category: Unreleased Structures

2018-03-21T06:01:47Z

New page: '''Unreleased structure''' The entry 5zhy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zhy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]