5zm8 - Revision history

OCA at 09:01, 22 November 2023

2023-11-22T09:01:04Z

←Older revision		Revision as of 09:01, 22 November 2023
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	==Crystal structure of ORP2-ORD in complex with PI(4,5)P2==		==Crystal structure of ORP2-ORD in complex with PI(4,5)P2==
-	<StructureSection load='5zm8' size='340' side='right' caption='[[5zm8]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5zm8' size='340' side='right'caption='[[5zm8]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zm8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZM8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZM8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEP:[(2~{S})-1-octadecanoyloxy-3-[oxidanyl-[(1~{R},2~{R},3~{S},4~{S},5~{S},6~{S})-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl]+icosa-5,8,11,14-tetraenoate'>IEP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OSBPL2, KIAA0772, ORP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IEP:[(2~{S})-1-octadecanoyloxy-3-[oxidanyl-[(1~{R},2~{R},3~{S},4~{S},5~{S},6~{S})-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl]+icosa-5,8,11,14-tetraenoate'>IEP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zm8 OCA], [~~http~~://pdbe.org/5zm8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zm8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zm8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zm8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zm8 OCA], [https://pdbe.org/5zm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zm8 RCSB], [https://www.ebi.ac.uk/pdbsum/5zm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zm8 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN]] Autosomal dominant non-syndromic sensorineural deafness type DFNA. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN] Autosomal dominant non-syndromic sensorineural deafness type DFNA. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN]] Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184). Binds 25-hydroxycholesterol (PubMed:17428193).<ref>PMID:11279184</ref> <ref>PMID:17428193</ref>	+	[https://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN] Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184). Binds 25-hydroxycholesterol (PubMed:17428193).<ref>PMID:11279184</ref> <ref>PMID:17428193</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Dong, J Q~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wang, H~~]]	+	[[Category: Dong JQ]]
-	[[Category: Wang~~, J~~]]	+	[[Category: Wang H]]
-	[[Category: ~~Wu,~~ J W]]	+	[[Category: Wang J]]
-	[[Category: ~~Complex]]~~	+	[[Category: Wu JW]]
-	~~[[Category: Lipid transport]]~~	+
-	~~[[Category: Phospholipid]]~~	+
-	~~[[Category: Transporter~~]]	+

OCA at 07:40, 21 February 2019

2019-02-21T07:40:14Z

←Older revision		Revision as of 07:40, 21 February 2019
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	<StructureSection load='5zm8' size='340' side='right' caption='[[5zm8]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5zm8' size='340' side='right' caption='[[5zm8]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zm8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zm8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZM8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEP:[(2~{S})-1-octadecanoyloxy-3-[oxidanyl-[(1~{R},2~{R},3~{S},4~{S},5~{S},6~{S})-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl]+icosa-5,8,11,14-tetraenoate'>IEP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEP:[(2~{S})-1-octadecanoyloxy-3-[oxidanyl-[(1~{R},2~{R},3~{S},4~{S},5~{S},6~{S})-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl]+icosa-5,8,11,14-tetraenoate'>IEP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OSBPL2, KIAA0772, ORP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zm8 OCA], [http://pdbe.org/5zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zm8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zm8 OCA], [http://pdbe.org/5zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zm8 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Dong, J Q]]		[[Category: Dong, J Q]]
	[[Category: Wang, H]]		[[Category: Wang, H]]

OCA at 06:08, 2 January 2019

2019-01-02T06:08:16Z

←Older revision		Revision as of 06:08, 2 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zm8 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of ORP2-ORD in complex with PI(4,5)P2==
		+	<StructureSection load='5zm8' size='340' side='right' caption='[[5zm8]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zm8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZM8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEP:[(2~{S})-1-octadecanoyloxy-3-[oxidanyl-[(1~{R},2~{R},3~{S},4~{S},5~{S},6~{S})-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl]+icosa-5,8,11,14-tetraenoate'>IEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zm8 OCA], [http://pdbe.org/5zm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zm8 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN]] Autosomal dominant non-syndromic sensorineural deafness type DFNA. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OSBL2_HUMAN OSBL2_HUMAN]] Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184). Binds 25-hydroxycholesterol (PubMed:17428193).<ref>PMID:11279184</ref> <ref>PMID:17428193</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cholesterol is highly enriched at the plasma membrane (PM), and lipid transfer proteins may deliver cholesterol to the PM in a nonvesicular manner. Here, through a mini-screen, we identified the oxysterol binding protein (OSBP)-related protein 2 (ORP2) as a novel mediator of selective cholesterol delivery to the PM. Interestingly, ORP2-mediated enrichment of PM cholesterol was coupled with the removal of phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2) from the PM. ORP2 overexpression or deficiency impacted the levels of PM cholesterol and PI(4,5)P2, and ORP2 efficiently transferred both cholesterol and PI(4,5)P2in vitro. We determined the structure of ORP2 in complex with PI(4,5)P2 at 2.7 A resolution. ORP2 formed a stable tetramer in the presence of PI(4,5)P2, and tetramerization was required for ORP2 to transfer PI(4,5)P2. Our results identify a novel pathway for cholesterol delivery to the PM and establish ORP2 as a key regulator of both cholesterol and PI(4,5)P2 of the PM.

-	~~Authors~~:	+	ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2).,Wang H, Ma Q, Qi Y, Dong J, Du X, Rae J, Wang J, Wu WF, Brown AJ, Parton RG, Wu JW, Yang H Mol Cell. 2018 Dec 10. pii: S1097-2765(18)30984-5. doi:, 10.1016/j.molcel.2018.11.014. PMID:30581148<ref>PMID:30581148</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5zm8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dong, J Q]]
		+	[[Category: Wang, H]]
		+	[[Category: Wang, J]]
		+	[[Category: Wu, J W]]
		+	[[Category: Complex]]
		+	[[Category: Lipid transport]]
		+	[[Category: Phospholipid]]
		+	[[Category: Transporter]]

OCA at 08:32, 5 September 2018

2018-09-05T08:32:45Z

←Older revision		Revision as of 08:32, 5 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zm8 is ON HOLD	+	The entry 5zm8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5zm8" [edit=sysop:move=sysop]

2018-04-11T12:20:34Z

Protected "5zm8" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:20, 11 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zm8 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-04-11T12:20:32Z

New page: '''Unreleased structure''' The entry 5zm8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zm8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]