6a4k - Revision history

OCA at 09:18, 22 November 2023

2023-11-22T09:18:08Z

←Older revision		Revision as of 09:18, 22 November 2023
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	<StructureSection load='6a4k' size='340' side='right'caption='[[6a4k]], [[Resolution\|resolution]] 3.15Å' scene=''>		<StructureSection load='6a4k' size='340' side='right'caption='[[6a4k]], [[Resolution\|resolution]] 3.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a4k]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A4K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a4k]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/California/7/2009(H1N1)) Influenza A virus (A/California/7/2009(H1N1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A4K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.15Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6a4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4k OCA], [~~http~~://pdbe.org/6a4k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a4k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a4k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4k ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4k OCA], [https://pdbe.org/6a4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a4k RCSB], [https://www.ebi.ac.uk/pdbsum/6a4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4k ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C3W5X2_9INFA C3W5X2_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[SAAS:SAAS00842036] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6a4k" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6a4k" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ko~~, T P~~]]	+	[[Category: Ko TP]]
-	[[Category: Lee~~, C C~~]]	+	[[Category: Lee CC]]
-	[[Category: Lin~~, L L~~]]	+	[[Category: Lin LL]]
-	[[Category: Wang~~, A H.J]]~~	+	[[Category: Wang AHJ]]
-	~~[[Category: Antibody fab]]~~	+
-	~~[[Category: Complex structure]]~~	+
-	~~[[Category: Influenza hemagglutinin]]~~	+
-	~~[[Category: Specific binding]]~~	+
-	~~[[Category: Viral protein-immune system complex~~]]	+

OCA at 06:31, 27 March 2019

2019-03-27T06:31:42Z

←Older revision		Revision as of 06:31, 27 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6a4k is ~~ON HOLD until Paper~~ Publication	+	==Human antibody 32D6 Fab in complex with H1N1 influenza A virus HA1==
		+	<StructureSection load='6a4k' size='340' side='right'caption='[[6a4k]], [[Resolution\|resolution]] 3.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a4k]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A4K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4k OCA], [http://pdbe.org/6a4k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a4k RCSB], [http://www.ebi.ac.uk/pdbsum/6a4k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4k ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza is a contagious acute respiratory disease caused by the influenza virus infection. Hemagglutinin (HA) is an important target in the therapeutic treatment and diagnostic detection of the influenza virus. Influenza A virus encompasses several different HA subtypes with different strains, which are constantly changing. In this study, we identified a fully human H1N1 neutralizing antibody (32D6) via an Epstein-Barr virus-immortalized B cell-based technology. 32D6 specifically neutralizes the clinically isolated H1N1 strains after the 2009 pandemic but not the earlier strains. The epitope was identified through X-ray crystallographic analysis of the 32D6-Fab/HA1 complex structure, which revealed a unique loop conformation located on the top surface of HA. The major region is composed of two peptide segments (residues 172-177 and 206-213), which form an abreast loop conformation. The residue T262 between the two loops forms a conformational epitope for recognition by 32D6. Three water molecules were observed at the interface of HA and the heavy chain, and they may constitute a stabilizing element for the 32D6-HA association. In addition, each 32D6-Fab is likely capable of blocking one HA trimer. This study provides important information on the strain specificity of 32D6 for the therapeutic treatment and detection of viral infection.

-	~~Authors~~:	+	An Effective Neutralizing Antibody Against Influenza Virus H1N1 from Human B Cells.,Lee CC, Yang CY, Lin LL, Ko TP, Chang AH, Chang SS, Wang AH Sci Rep. 2019 Mar 14;9(1):4546. doi: 10.1038/s41598-019-40937-4. PMID:30872685<ref>PMID:30872685</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6a4k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ko, T P]]
		+	[[Category: Lee, C C]]
		+	[[Category: Lin, L L]]
		+	[[Category: Wang, A H.J]]
		+	[[Category: Antibody fab]]
		+	[[Category: Complex structure]]
		+	[[Category: Influenza hemagglutinin]]
		+	[[Category: Specific binding]]
		+	[[Category: Viral protein-immune system complex]]

OCA at 05:31, 4 July 2018

2018-07-04T05:31:12Z

←Older revision		Revision as of 05:31, 4 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6a4k is ON HOLD	+	The entry 6a4k is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6a4k" [edit=sysop:move=sysop]

2018-06-27T03:54:02Z

Protected "6a4k" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:54, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6a4k is ON HOLD Authors: Description: Category: Unreleased Structures

2018-06-27T03:54:01Z

New page: '''Unreleased structure''' The entry 6a4k is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6a4k is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]