6aa2 - Revision history

OCA at 09:22, 22 November 2023

2023-11-22T09:22:03Z

←Older revision		Revision as of 09:22, 22 November 2023
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	<StructureSection load='6aa2' size='340' side='right'caption='[[6aa2]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6aa2' size='340' side='right'caption='[[6aa2]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aa2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AA2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AA2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aa2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AA2 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6aa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aa2 OCA], [~~http~~://pdbe.org/6aa2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6aa2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6aa2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6aa2 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aa2 OCA], [https://pdbe.org/6aa2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aa2 RCSB], [https://www.ebi.ac.uk/pdbsum/6aa2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aa2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.	+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6aa2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6aa2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hisabori, T]]	+	[[Category: Hisabori T]]
-	[[Category: Kurisu, G]]	+	[[Category: Kurisu G]]
-	[[Category: Sugiura, K]]	+	[[Category: Sugiura K]]
-	[[Category: Tabushi, N]]	+	[[Category: Tabushi N]]
-	[[Category: Tanaka, H]]	+	[[Category: Tanaka H]]
-	[[Category: Yasuda, A]]	+	[[Category: Yasuda A]]
-	~~[[Category: Fluorescent indicator protein]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Redox status]]~~	+
-	~~[[Category: Yfp~~]]	+

OCA at 06:02, 29 May 2019

2019-05-29T06:02:51Z

←Older revision		Revision as of 06:02, 29 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6aa2 is ~~ON HOLD until Paper~~ Publication	+	==X-ray structure of ReQy1 (oxidized form)==
		+	<StructureSection load='6aa2' size='340' side='right'caption='[[6aa2]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6aa2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AA2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AA2 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aa2 OCA], [http://pdbe.org/6aa2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aa2 RCSB], [http://www.ebi.ac.uk/pdbsum/6aa2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aa2 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Change in the intracellular redox state is a consequence of various metabolic reactions, which simultaneously regulates various physiological phenomena in cells. Monitoring the redox state in living cells is thus very important for understanding cellular physiology. Various genetically encoded fluorescent redox sensors have therefore been developed. Recently, we developed oxidation-sensitive fluorescent proteins named Oba-Q (Sugiura, K., et al. (2015) Biochem. Biophys. Res. Commun. 457, 242-248), which exhibit dramatic quenching under oxidizing conditions. To extend the range of uses of redox sensor proteins, we refined these proteins based on the molecular architecture applied to Oba-Q, and successfully produced several redox sensor proteins based on CFP and YFP. Interestingly, some of these sensor proteins showed the reverse changes in emission compared with Oba-Q, implying remarkable fluorescence quenching under reducing conditions. We named this type of sensor protein Re-Q, reduction-sensed quenching protein. The cause of the redox-dependent fluorescence quenching could be clearly explained based on the crystal structure of Re-Q in the reduced and oxidized forms. In addition, by introducing suitable mutations into the sensors, we produced Oba-Q and Re-Q mutants exhibiting various midpoint redox potentials. This series of proteins can cover a wide range of redox potentials in the cell, so they should be applicable to various cells and even intracellular organelles. As an example, we successfully measured the redox responses in different cell compartments of cultured mammalian cells simultaneously against the anticancer reagents Kp372-1.

-	~~Authors: Sugiura~~, K~~., Yasuda, A., Tabushi, N.~~, Tanaka, H., Kurisu, G., ~~Hisabori~~, T.	+	Multicolor redox sensor proteins can visualize redox changes in various compartments of the living cell.,Sugiura K, Tanaka H, Kurisu G, Wakabayashi KI, Hisabori T Biochim Biophys Acta Gen Subj. 2019 Jun;1863(6):1098-1107. doi:, 10.1016/j.bbagen.2019.01.016. Epub 2019 Apr 4. PMID:30953671<ref>PMID:30953671</ref>

-	~~Description: X-ray structure~~ of ~~ReQy1 (oxidized form)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6aa2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hisabori, T]]		[[Category: Hisabori, T]]
-	[[Category: Tanaka, H]]
	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]
	[[Category: Sugiura, K]]		[[Category: Sugiura, K]]
-	[[Category: Yasuda, A]]
	[[Category: Tabushi, N]]		[[Category: Tabushi, N]]
		+	[[Category: Tanaka, H]]
		+	[[Category: Yasuda, A]]
		+	[[Category: Fluorescent indicator protein]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Redox status]]
		+	[[Category: Yfp]]

OCA at 05:05, 31 October 2018

2018-10-31T05:05:00Z

←Older revision		Revision as of 05:05, 31 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6aa2 is ON HOLD	+	The entry 6aa2 is ON HOLD until Paper Publication

	Authors: Sugiura, K., Yasuda, A., Tabushi, N., Tanaka, H., Kurisu, G., Hisabori, T.		Authors: Sugiura, K., Yasuda, A., Tabushi, N., Tanaka, H., Kurisu, G., Hisabori, T.
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	Description: X-ray structure of ReQy1 (oxidized form)		Description: X-ray structure of ReQy1 (oxidized form)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Yasuda, A]]
	[[Category: Hisabori, T]]		[[Category: Hisabori, T]]
	[[Category: Tanaka, H]]		[[Category: Tanaka, H]]
	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]
-	[[Category: Tabushi, N]]
	[[Category: Sugiura, K]]		[[Category: Sugiura, K]]
		+	[[Category: Yasuda, A]]
		+	[[Category: Tabushi, N]]

OCA: Protected "6aa2" [edit=sysop:move=sysop]

2018-07-25T05:42:56Z

Protected "6aa2" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:42, 25 July 2018

OCA: New page: '''Unreleased structure''' The entry 6aa2 is ON HOLD Authors: Sugiura, K., Yasuda, A., Tabushi, N., Tanaka, H., Kurisu, G., Hisabori, T. Description: X-ray structure of ReQy1 (oxidized...

2018-07-25T05:42:55Z

New page: '''Unreleased structure''' The entry 6aa2 is ON HOLD Authors: Sugiura, K., Yasuda, A., Tabushi, N., Tanaka, H., Kurisu, G., Hisabori, T. Description: X-ray structure of ReQy1 (oxidized...

New page

'''Unreleased structure'''

The entry 6aa2 is ON HOLD

Authors: Sugiura, K., Yasuda, A., Tabushi, N., Tanaka, H., Kurisu, G., Hisabori, T.

Description: X-ray structure of ReQy1 (oxidized form)
[[Category: Unreleased Structures]]
[[Category: Yasuda, A]]
[[Category: Hisabori, T]]
[[Category: Tanaka, H]]
[[Category: Kurisu, G]]
[[Category: Tabushi, N]]
[[Category: Sugiura, K]]