6ahp - Revision history

OCA at 10:31, 27 March 2024

2024-03-27T10:31:54Z

←Older revision		Revision as of 10:31, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6ahp' size='340' side='right'caption='[[6ahp]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6ahp' size='340' side='right'caption='[[6ahp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ahp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"oceanomonas_alginolytica"_miyamoto_et_al._1961 "oceanomonas alginolytica" miyamoto et al. 1961~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AHP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ahp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AHP FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~6ahq~~\|~~6ahq~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahp OCA], [~~http~~://pdbe.org/6ahp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ahp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ahp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahp OCA], [https://pdbe.org/6ahp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ahp RCSB], [https://www.ebi.ac.uk/pdbsum/6ahp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahp ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A0P7ET86_VIBAL A0A0P7ET86_VIBAL] Controls the rotational direction of flagella during chemotaxis.[ARBA:ARBA00002254][RuleBase:RU364125]
-	~~Many motile bacteria swim or swarm using a filamentous rotating organelle, the flagellum~~. ~~FliL, a component protein of the flagellar motor, is known to enhance the motor performance under high-load conditions in some bacteria~~. ~~Here we determined the structure of the periplasmic region of FliL (FliLPeri) of the polar flagellum of Vibrio alginolyticus FliLPeri shows a remarkable structural similarity to the stomatin~~/~~prohibitin~~/~~flotillin/HflK/C (SPFH) domain of stomatin family proteins, some of which are involved in modulation of ion channel activities in various organisms. FliLPeri forms a ring assembly in~~ the ~~crystal with an inner diameter~~ of around 8 nm, which is comparable to the size of the stator unit. Mutational analyses suggest that the FliL ring forms a complex with the stator unit and that the length of the periplasmic linkers of FliL and the stator B-subunit is essential for the complex formation. We propose a model of the FliL-stator complex to discuss how Vibrio FliL modulates stator function in the bacterial flagellar motor under conditions of high viscosity.IMPORTANCE Some flagellated bacteria regulate motor torque in response to the external load change. This behavior is critical for survival, but the mechanism has remained unknown. Here, we focused on a key protein, FliL of Vibrio alginolyticus, and solved the crystal structure of its periplasmic region (FliLPeri). FliLPeri reveals striking structural similarity to a conserved domain of stomatin, which is involved in ion channel regulation in some organisms, including mammals. FliLPeri forms a ring with an inner diameter that is comparable in size to the stator unit. The mutational analyses suggested that the presence of the ring-like assembly of FliL around the stator unit enhances the surface swarming of Vibrio cells. Our study data also imply that the structural element for the ion channel regulation is conserved from bacteria to mammals.	+

-	Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function.,Takekawa N, Isumi M, Terashima H, Zhu S, Nishino Y, Sakuma M, Kojima S, Homma M, Imada K MBio. 2019 Mar 19;10(2). pii: mBio.00292-19. doi: 10.1128/mBio.00292-19. PMID:30890608<ref>PMID:30890608</ref>	+	==See Also==
-		+	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6ahp" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Oceanomonas alginolytica miyamoto et al. 1961]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Homma, M]]	+	[[Category: Vibrio alginolyticus]]
-	[[Category: Imada, K]]	+	[[Category: Homma M]]
-	[[Category: Isumi, M]]	+	[[Category: Imada K]]
-	[[Category: Kojima, S]]	+	[[Category: Isumi M]]
-	[[Category: Sakuma, M]]	+	[[Category: Kojima S]]
-	[[Category: Takekawa, N]]	+	[[Category: Sakuma M]]
-	~~[[Category: Flagellar motor protein]]~~	+	[[Category: Takekawa N]]
-	~~[[Category: Motor protein~~]]	+

OCA at 08:07, 3 April 2019

2019-04-03T08:07:31Z

←Older revision		Revision as of 08:07, 3 April 2019
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	==Structure of the 58-167 fragment of FliL==		==Structure of the 58-167 fragment of FliL==
-	<StructureSection load='6ahp' size='340' side='right' caption='[[6ahp]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='6ahp' size='340' side='right'caption='[[6ahp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ahp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AHP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ahp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"oceanomonas_alginolytica"_miyamoto_et_al._1961 "oceanomonas alginolytica" miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AHP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ahq\|6ahq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ahq\|6ahq]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahp OCA], [http://pdbe.org/6ahp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ahp RCSB], [http://www.ebi.ac.uk/pdbsum/6ahp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahp OCA], [http://pdbe.org/6ahp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ahp RCSB], [http://www.ebi.ac.uk/pdbsum/6ahp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahp ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many motile bacteria swim or swarm using a filamentous rotating organelle, the flagellum. FliL, a component protein of the flagellar motor, is known to enhance the motor performance under high-load conditions in some bacteria. Here we determined the structure of the periplasmic region of FliL (FliLPeri) of the polar flagellum of Vibrio alginolyticus FliLPeri shows a remarkable structural similarity to the stomatin/prohibitin/flotillin/HflK/C (SPFH) domain of stomatin family proteins, some of which are involved in modulation of ion channel activities in various organisms. FliLPeri forms a ring assembly in the crystal with an inner diameter of around 8 nm, which is comparable to the size of the stator unit. Mutational analyses suggest that the FliL ring forms a complex with the stator unit and that the length of the periplasmic linkers of FliL and the stator B-subunit is essential for the complex formation. We propose a model of the FliL-stator complex to discuss how Vibrio FliL modulates stator function in the bacterial flagellar motor under conditions of high viscosity.IMPORTANCE Some flagellated bacteria regulate motor torque in response to the external load change. This behavior is critical for survival, but the mechanism has remained unknown. Here, we focused on a key protein, FliL of Vibrio alginolyticus, and solved the crystal structure of its periplasmic region (FliLPeri). FliLPeri reveals striking structural similarity to a conserved domain of stomatin, which is involved in ion channel regulation in some organisms, including mammals. FliLPeri forms a ring with an inner diameter that is comparable in size to the stator unit. The mutational analyses suggested that the presence of the ring-like assembly of FliL around the stator unit enhances the surface swarming of Vibrio cells. Our study data also imply that the structural element for the ion channel regulation is conserved from bacteria to mammals.
		+
		+	Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function.,Takekawa N, Isumi M, Terashima H, Zhu S, Nishino Y, Sakuma M, Kojima S, Homma M, Imada K MBio. 2019 Mar 19;10(2). pii: mBio.00292-19. doi: 10.1128/mBio.00292-19. PMID:30890608<ref>PMID:30890608</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ahp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Oceanomonas alginolytica miyamoto et al. 1961]]
		+	[[Category: Large Structures]]
	[[Category: Homma, M]]		[[Category: Homma, M]]
	[[Category: Imada, K]]		[[Category: Imada, K]]

OCA at 07:24, 6 March 2019

2019-03-06T07:24:03Z

←Older revision		Revision as of 07:24, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ahp ~~is ON HOLD~~ ~~until Paper Publication~~	+	==Structure of the 58-167 fragment of FliL==
-		+	<StructureSection load='6ahp' size='340' side='right' caption='[[6ahp]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ahp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AHP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ahq\|6ahq]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ahp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ahp OCA], [http://pdbe.org/6ahp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ahp RCSB], [http://www.ebi.ac.uk/pdbsum/6ahp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ahp ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Homma, M]]
		+	[[Category: Imada, K]]
		+	[[Category: Isumi, M]]
		+	[[Category: Kojima, S]]
		+	[[Category: Sakuma, M]]
		+	[[Category: Takekawa, N]]
		+	[[Category: Flagellar motor protein]]
		+	[[Category: Motor protein]]

OCA at 06:09, 3 October 2018

2018-10-03T06:09:18Z

←Older revision		Revision as of 06:09, 3 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ahp is ON HOLD	+	The entry 6ahp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ahp" [edit=sysop:move=sysop]

2018-08-29T05:32:45Z

Protected "6ahp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 29 August 2018

OCA: New page: '''Unreleased structure''' The entry 6ahp is ON HOLD Authors: Description: Category: Unreleased Structures

2018-08-29T05:32:44Z

New page: '''Unreleased structure''' The entry 6ahp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ahp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]