6akf - Revision history

OCA at 09:35, 22 November 2023

2023-11-22T09:35:54Z

←Older revision		Revision as of 09:35, 22 November 2023
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	==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin==		==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin==
-	<StructureSection load='6akf' size='340' side='right' caption='[[6akf]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='6akf' size='340' side='right'caption='[[6akf]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6akf]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus~~ perfringens~~" veillon and zuber 1898~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AKF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6akf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AKF FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6ake\|6ake]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Cldn3 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice]), cpe ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [https://pdbe.org/6akf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [https://www.ebi.ac.uk/pdbsum/6akf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [~~http~~://pdbe.org/6akf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6akf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE]] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> ~~[[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning.~~	+	[https://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ perfringens ~~veillon and zuber 1898~~]]	+	[[Category: Clostridium perfringens]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fujiyoshi, Y~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Irie, K~~]]	+	[[Category: Fujiyoshi Y]]
-	[[Category: ~~Nakamura, S~~]]	+	[[Category: Irie K]]
-	[[Category: ~~Cell adhesion]]~~	+	[[Category: Nakamura S]]
-	~~[[Category: Membrane protein-toxin complex]]~~	+
-	~~[[Category: Tight junction~~]]	+

OCA at 08:19, 6 March 2019

2019-03-06T08:19:22Z

←Older revision		Revision as of 08:19, 6 March 2019
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	==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin==		==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin==
-	<StructureSection load='6akf' size='340' side='right' caption='[[6akf]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='6akf' size='340' side='right' caption='[[6akf]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6akf]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6akf]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ake\|6ake]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ake\|6ake]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cldn3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), cpe ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [http://pdbe.org/6akf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [http://www.ebi.ac.uk/pdbsum/6akf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [http://pdbe.org/6akf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [http://www.ebi.ac.uk/pdbsum/6akf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE]] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> [[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning.		[[http://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE]] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> [[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tight junction is a cell adhesion apparatus functioning as barrier and/or channel in the paracellular spaces of epithelia. Claudin is the major component of tight junction and polymerizes to form tight junction strands with various morphologies that may correlate with their functions. Here we present the crystal structure of mammalian claudin-3 at 3.6 A resolution. The third transmembrane helix of claudin-3 is clearly bent compared with that of other subtypes. Structural analysis of additional two mutants with a single mutation representing other subtypes in the third helix indicates that this helix takes a bent or straight structure depending on the residue. The presence or absence of the helix bending changes the positions of residues related to claudin-claudin interactions and affects the morphology and adhesiveness of the tight junction strands. These results evoke a model for tight junction strand formation with different morphologies - straight or curvy strands - observed in native epithelia.
		+
		+	Morphologic determinant of tight junctions revealed by claudin-3 structures.,Nakamura S, Irie K, Tanaka H, Nishikawa K, Suzuki H, Saitoh Y, Tamura A, Tsukita S, Fujiyoshi Y Nat Commun. 2019 Feb 18;10(1):816. doi: 10.1038/s41467-019-08760-7. PMID:30778075<ref>PMID:30778075</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6akf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus perfringens veillon and zuber 1898]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Fujiyoshi, Y]]		[[Category: Fujiyoshi, Y]]
	[[Category: Irie, K]]		[[Category: Irie, K]]

OCA at 06:36, 21 February 2019

2019-02-21T06:36:43Z

←Older revision		Revision as of 06:36, 21 February 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6akf is ON HOLD until Paper Publication~~	+	==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin==
-		+	<StructureSection load='6akf' size='340' side='right' caption='[[6akf]], [[Resolution\|resolution]] 3.90Å' scene=''>
-	~~Authors: Nakamura, S., Irie, K., Fujiyoshi, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6akf]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKF FirstGlance]. <br>
-	~~Description:~~ Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ake\|6ake]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [http://pdbe.org/6akf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [http://www.ebi.ac.uk/pdbsum/6akf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE]] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> [[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning.
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fujiyoshi, Y]]		[[Category: Fujiyoshi, Y]]
	[[Category: Irie, K]]		[[Category: Irie, K]]
	[[Category: Nakamura, S]]		[[Category: Nakamura, S]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Membrane protein-toxin complex]]
		+	[[Category: Tight junction]]

OCA at 06:05, 26 September 2018

2018-09-26T06:05:04Z

←Older revision		Revision as of 06:05, 26 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6akf is ON HOLD	+	The entry 6akf is ON HOLD until Paper Publication

	Authors: Nakamura, S., Irie, K., Fujiyoshi, Y.		Authors: Nakamura, S., Irie, K., Fujiyoshi, Y.

OCA: Protected "6akf" [edit=sysop:move=sysop]

2018-09-05T08:39:28Z

Protected "6akf" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:39, 5 September 2018

OCA: New page: '''Unreleased structure''' The entry 6akf is ON HOLD Authors: Nakamura, S., Irie, K., Fujiyoshi, Y. Description: Crystal structure of mouse claudin-3 P134A mutant in complex with C-ter...

2018-09-05T08:39:27Z

New page: '''Unreleased structure''' The entry 6akf is ON HOLD Authors: Nakamura, S., Irie, K., Fujiyoshi, Y. Description: Crystal structure of mouse claudin-3 P134A mutant in complex with C-ter...

New page

'''Unreleased structure'''

The entry 6akf is ON HOLD

Authors: Nakamura, S., Irie, K., Fujiyoshi, Y.

Description: Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin
[[Category: Unreleased Structures]]
[[Category: Fujiyoshi, Y]]
[[Category: Irie, K]]
[[Category: Nakamura, S]]