6aly - Revision history

OCA at 10:37, 14 June 2023

2023-06-14T10:37:07Z

←Older revision		Revision as of 10:37, 14 June 2023
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	==Solution structure of yeast Med15 ABD2 residues 277-368==		==Solution structure of yeast Med15 ABD2 residues 277-368==
-	<StructureSection load='6aly' size='340' side='right'caption='[[6aly~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6aly' size='340' side='right'caption='[[6aly]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ALY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ALY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aly OCA], [~~http~~://pdbe.org/6aly PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6aly RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6aly PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6aly ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aly OCA], [https://pdbe.org/6aly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aly RCSB], [https://www.ebi.ac.uk/pdbsum/6aly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aly ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>	+	[https://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hahn, S]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Klevit~~, R E~~]]	+	[[Category: Hahn S]]
-	[[Category: Pacheco, D]]	+	[[Category: Klevit RE]]
-	[[Category: Tuttle~~, L M~~]]	+	[[Category: Pacheco D]]
-	[[Category: Warfield, L]]	+	[[Category: Tuttle LM]]
-	~~[[Category: Abd]]~~	+	[[Category: Warfield L]]
-	~~[[Category: Gal11]]~~	+
-	~~[[Category: Helical]]~~	+
-	~~[[Category: Med15]]~~	+
-	~~[[Category: Mediator]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription activation]]~~	+
-	~~[[Category: Yeast~~]]	+

OCA at 10:03, 1 January 2020

2020-01-01T10:03:31Z

←Older revision		Revision as of 10:03, 1 January 2020
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	==Solution structure of yeast Med15 ABD2 residues 277-368==		==Solution structure of yeast Med15 ABD2 residues 277-368==
-	<StructureSection load='6aly' size='340' side='right' caption='[[6aly]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='6aly' size='340' side='right'caption='[[6aly]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ALY FirstGlance]. <br>		<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ALY FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Hahn, S]]		[[Category: Hahn, S]]
	[[Category: Klevit, R E]]		[[Category: Klevit, R E]]

OCA at 07:41, 21 February 2019

2019-02-21T07:41:43Z

←Older revision		Revision as of 07:41, 21 February 2019
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6aly" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6aly" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mediator\|Mediator]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:53, 4 April 2018

2018-04-04T06:53:42Z

←Older revision		Revision as of 06:53, 4 April 2018
Line 3:		Line 3:
	<StructureSection load='6aly' size='340' side='right' caption='[[6aly]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='6aly' size='340' side='right' caption='[[6aly]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ALY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ALY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aly OCA], [http://pdbe.org/6aly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aly RCSB], [http://www.ebi.ac.uk/pdbsum/6aly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aly ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aly OCA], [http://pdbe.org/6aly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aly RCSB], [http://www.ebi.ac.uk/pdbsum/6aly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aly ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>		[[http://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transcription activation domains (ADs) are inherently disordered proteins that often target multiple coactivator complexes, but the specificity of these interactions is not understood. Efficient transcription activation by yeast Gcn4 requires its tandem ADs and four activator-binding domains (ABDs) on its target, the Mediator subunit Med15. Multiple ABDs are a common feature of coactivator complexes. We find that the large Gcn4-Med15 complex is heterogeneous and contains nearly all possible AD-ABD interactions. Gcn4-Med15 forms via a dynamic fuzzy protein-protein interface, where ADs bind the ABDs in multiple orientations via hydrophobic regions that gain helicity. This combinatorial mechanism allows individual low-affinity and specificity interactions to generate a biologically functional, specific, and higher affinity complex despite lacking a defined protein-protein interface. This binding strategy is likely representative of many activators that target multiple coactivators, as it allows great flexibility in combinations of activators that can cooperate to regulate genes with variable coactivator requirements.
		+
		+	Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex.,Tuttle LM, Pacheco D, Warfield L, Luo J, Ranish J, Hahn S, Klevit RE Cell Rep. 2018 Mar 20;22(12):3251-3264. doi: 10.1016/j.celrep.2018.02.097. PMID:29562181<ref>PMID:29562181</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6aly" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Hahn, S]]		[[Category: Hahn, S]]
	[[Category: Klevit, R E]]		[[Category: Klevit, R E]]

OCA at 07:37, 21 March 2018

2018-03-21T07:37:12Z

←Older revision		Revision as of 07:37, 21 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6aly is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Solution structure of yeast Med15 ABD2 residues 277-368==
-		+	<StructureSection load='6aly' size='340' side='right' caption='[[6aly]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6aly]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ALY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ALY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aly OCA], [http://pdbe.org/6aly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aly RCSB], [http://www.ebi.ac.uk/pdbsum/6aly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aly ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hahn, S]]
		+	[[Category: Klevit, R E]]
		+	[[Category: Pacheco, D]]
		+	[[Category: Tuttle, L M]]
		+	[[Category: Warfield, L]]
		+	[[Category: Abd]]
		+	[[Category: Gal11]]
		+	[[Category: Helical]]
		+	[[Category: Med15]]
		+	[[Category: Mediator]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription activation]]
		+	[[Category: Yeast]]

OCA at 05:26, 11 October 2017

2017-10-11T05:26:19Z

←Older revision		Revision as of 05:26, 11 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6aly is ON HOLD	+	The entry 6aly is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6aly" [edit=sysop:move=sysop]

2017-09-27T16:32:58Z

Protected "6aly" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:32, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6aly is ON HOLD Authors: Description: Category: Unreleased Structures

2017-09-27T16:32:57Z

New page: '''Unreleased structure''' The entry 6aly is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6aly is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]