6b1n - Revision history

OCA at 14:30, 4 October 2023

2023-10-04T14:30:31Z

←Older revision		Revision as of 14:30, 4 October 2023
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	<StructureSection load='6b1n' size='340' side='right'caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6b1n' size='340' side='right'caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B1N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B1N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1n OCA], [~~http~~://pdbe.org/6b1n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b1n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b1n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1n ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1n OCA], [https://pdbe.org/6b1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b1n RCSB], [https://www.ebi.ac.uk/pdbsum/6b1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>	+	[https://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Donnell, J P.~~O]]	+	[[Category: O'Donnell JP]]
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Hsp70 family member~~]]	+

OCA at 10:11, 1 January 2020

2020-01-01T10:11:16Z

←Older revision		Revision as of 10:11, 1 January 2020
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	==Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant==		==Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant==
-	<StructureSection load='6b1n' size='340' side='right' caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='6b1n' size='340' side='right'caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1N FirstGlance]. <br>		<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1N FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6b1n" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6b1n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Donnell, J P.O]]		[[Category: Donnell, J P.O]]
	[[Category: Atpase]]		[[Category: Atpase]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Hsp70 family member]]		[[Category: Hsp70 family member]]

OCA at 07:14, 28 February 2018

2018-02-28T07:14:50Z

←Older revision		Revision as of 07:14, 28 February 2018
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	<StructureSection load='6b1n' size='340' side='right' caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6b1n' size='340' side='right' caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1n OCA], [http://pdbe.org/6b1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1n RCSB], [http://www.ebi.ac.uk/pdbsum/6b1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1n OCA], [http://pdbe.org/6b1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1n RCSB], [http://www.ebi.ac.uk/pdbsum/6b1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1n ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Donnell, J P.O]]		[[Category: Donnell, J P.O]]
	[[Category: Atpase]]		[[Category: Atpase]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Hsp70 family member]]		[[Category: Hsp70 family member]]

OCA at 06:59, 17 January 2018

2018-01-17T06:59:40Z

←Older revision		Revision as of 06:59, 17 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6b1n is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant==
		+	<StructureSection load='6b1n' size='340' side='right' caption='[[6b1n]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6b1n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1n OCA], [http://pdbe.org/6b1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1n RCSB], [http://www.ebi.ac.uk/pdbsum/6b1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1n ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HSP7C_HUMAN HSP7C_HUMAN]] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.<ref>PMID:10722728</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ATPase domain of the 70-kD heat shock protein (Hsp70) family members shows a high degree of sequence, structural, and functional homology across species. A broadly conserved residue within the Hsp70 ATPase domain that captured our attention is an unpaired cysteine, positioned proximal to the site of nucleotide binding. Prior studies of several Hsp70 family members show this cysteine is not required for Hsp70 ATPase activity, yet select amino acid replacements of the cysteine can dramatically alter ATP hydrolysis. Moreover, post-translational modification of the cysteine has been reported to limit ATP hydrolysis for several Hsp70s. To better understand the underlying mechanism for how perturbation of this non-catalytic residue modulates Hsp70 function, we solved the structure for a cysteine-to-tryptophan mutation in the constitutively-expressed mammalian Hsp70 family member Hsc70. Our work reveals that the steric hindrance produced by a cysteine-to-tryptophan mutation disrupts the hydrogen-bond network within the active site resulting in a loss of proper catalytic magnesium coordination. We propose that a similarly altered active site is likely observed upon post-translational oxidation. We speculate that the subtle changes we detect in the hydrogen-bonding network may relate to the previously reported observation that cysteine oxidation can influence Hsp70 inter-domain communication.

-	~~Authors~~:	+	Disrupted hydrogen bond network and impaired ATPase activity in an Hsc70 cysteine mutant.,O'Donnell JP, Marsh HM, Sondermann H, Sevier C Biochemistry. 2018 Jan 4. doi: 10.1021/acs.biochem.7b01005. PMID:29300467<ref>PMID:29300467</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6b1n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Donnell, J P.O]]
		+	[[Category: Atpase]]
		+	[[Category: Chaperone]]
		+	[[Category: Hsp70 family member]]

OCA at 08:10, 4 October 2017

2017-10-04T08:10:51Z

←Older revision		Revision as of 08:10, 4 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b1n is ON HOLD	+	The entry 6b1n is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6b1n" [edit=sysop:move=sysop]

2017-09-27T17:04:17Z

Protected "6b1n" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:04, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6b1n is ON HOLD Authors: Description: Category: Unreleased Structures

2017-09-27T17:04:16Z

New page: '''Unreleased structure''' The entry 6b1n is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6b1n is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]