6b2n - Revision history

OCA at 14:31, 4 October 2023

2023-10-04T14:31:44Z

←Older revision		Revision as of 14:31, 4 October 2023
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	<StructureSection load='6b2n' size='340' side='right'caption='[[6b2n]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6b2n' size='340' side='right'caption='[[6b2n]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b2n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B2N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6B2N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b2n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B2N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B2N FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6b2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b2n OCA], [~~http~~://pdbe.org/6b2n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b2n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b2n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b2n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b2n OCA], [https://pdbe.org/6b2n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b2n RCSB], [https://www.ebi.ac.uk/pdbsum/6b2n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b2n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.	+	[https://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-lactamase~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jimah~~, J R~~]]	+	[[Category: Jimah JR]]
-	[[Category: Tolia~~, N H]]~~	+	[[Category: Tolia NH]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 21:35, 28 October 2020

2020-10-28T21:35:45Z

←Older revision		Revision as of 21:35, 28 October 2020
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	==Crystal structure of TEM-1 beta-lactamase mutant M182N==		==Crystal structure of TEM-1 beta-lactamase mutant M182N==
-	<StructureSection load='6b2n' size='340' side='right' caption='[[6b2n]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6b2n' size='340' side='right'caption='[[6b2n]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b2n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B2N OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B2N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b2n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B2N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6B2N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b2n OCA], [http://pdbe.org/6b2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b2n RCSB], [http://www.ebi.ac.uk/pdbsum/6b2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b2n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6b2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b2n OCA], [http://pdbe.org/6b2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b2n RCSB], [http://www.ebi.ac.uk/pdbsum/6b2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b2n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 6b2n" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6b2n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Jimah, J R]]		[[Category: Jimah, J R]]
	[[Category: Tolia, N H]]		[[Category: Tolia, N H]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:59, 17 January 2018

2018-01-17T06:59:46Z

←Older revision		Revision as of 06:59, 17 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6b2n is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of TEM-1 beta-lactamase mutant M182N==
		+	<StructureSection load='6b2n' size='340' side='right' caption='[[6b2n]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6b2n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B2N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b2n OCA], [http://pdbe.org/6b2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b2n RCSB], [http://www.ebi.ac.uk/pdbsum/6b2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b2n ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein stabilization is fundamental to enzyme function and evolution, yet understanding the determinants of a protein's stability remains a challenge. This is largely due to a shortage of atomically detailed models for the ensemble of relevant protein conformations and their relative populations. For example, the M182T substitution in TEM beta-lactamase, an enzyme that confers antibiotic resistance to bacteria, is stabilizing but the precise mechanism remains unclear. Here, we employ Markov state models (MSMs) to uncover how M182T shifts the distribution of different structures that TEM adopts. We find that M182T stabilizes a helix that is a key component of a domain interface. We then predict the effects of other mutations, including a novel stabilizing mutation, and experimentally test our predictions using a combination of stability measurements, crystallography, NMR, and in vivo measurements of bacterial fitness. We expect our insights and methodology to provide a valuable foundation for protein design.

-	~~Authors~~:	+	Prediction of New Stabilizing Mutations Based on Mechanistic Insights from Markov State Models.,Zimmerman MI, Hart KM, Sibbald CA, Frederick TE, Jimah JR, Knoverek CR, Tolia NH, Bowman GR ACS Cent Sci. 2017 Dec 27;3(12):1311-1321. doi: 10.1021/acscentsci.7b00465. Epub , 2017 Nov 21. PMID:29296672<ref>PMID:29296672</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6b2n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-lactamase]]
		+	[[Category: Jimah, J R]]
		+	[[Category: Tolia, N H]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Hydrolase]]

OCA at 05:44, 25 October 2017

2017-10-25T05:44:26Z

←Older revision		Revision as of 05:44, 25 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b2n is ON HOLD	+	The entry 6b2n is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6b2n" [edit=sysop:move=sysop]

2017-09-27T17:06:39Z

Protected "6b2n" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:06, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6b2n is ON HOLD Authors: Description: Category: Unreleased Structures

2017-09-27T17:06:38Z

New page: '''Unreleased structure''' The entry 6b2n is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6b2n is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]