6b4m - Revision history

OCA at 14:32, 4 October 2023

2023-10-04T14:32:36Z

←Older revision		Revision as of 14:32, 4 October 2023
Line 1:		Line 1:

	==Structural characterization of a novel monotreme-specific protein from the milk of the platypus==		==Structural characterization of a novel monotreme-specific protein from the milk of the platypus==
-	<StructureSection load='6b4m' size='340' side='right' caption='[[6b4m]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='6b4m' size='340' side='right'caption='[[6b4m]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b4m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Duckbill_platypus Duckbill platypus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B4M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b4m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ornithorhynchus_anatinus Ornithorhynchus anatinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B4M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~>, ~~<scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[4v00\|4v00]], [[4v3j\|4v3j]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">MLP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9258 Duckbill platypus])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4m OCA], [https://pdbe.org/6b4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b4m RCSB], [https://www.ebi.ac.uk/pdbsum/6b4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4m ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4m OCA], [~~http~~://pdbe.org/6b4m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b4m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b4m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4m ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/F6UME2_ORNAN F6UME2_ORNAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Duckbill platypus~~]]	+	[[Category: Large Structures]]
-	[[Category: Adams~~, T E~~]]	+	[[Category: Ornithorhynchus anatinus]]
-	[[Category: Kumar, A]]	+	[[Category: Adams TE]]
-	[[Category: Newman, J]]	+	[[Category: Kumar A]]
-	[[Category: Nicholas~~, K R~~]]	+	[[Category: Newman J]]
-	[[Category: Peat~~, T S~~]]	+	[[Category: Nicholas KR]]
-	[[Category: Sharp~~, J A]]~~	+	[[Category: Peat TS]]
-	~~[[Category: Antimicrobial protein]]~~	+	[[Category: Sharp JA]]
-	~~[[Category: Monotreme]]~~	+
-	~~[[Category: Novel-fold~~]]	+

OCA at 05:57, 16 May 2018

2018-05-16T05:57:14Z

←Older revision		Revision as of 05:57, 16 May 2018
Line 3:		Line 3:
	<StructureSection load='6b4m' size='340' side='right' caption='[[6b4m]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='6b4m' size='340' side='right' caption='[[6b4m]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b4m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B4M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b4m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Duckbill_platypus Duckbill platypus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B4M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4v00\|4v00]], [[4v3j\|4v3j]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4v00\|4v00]], [[4v3j\|4v3j]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MLP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9258 Duckbill platypus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4m OCA], [http://pdbe.org/6b4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b4m RCSB], [http://www.ebi.ac.uk/pdbsum/6b4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4m OCA], [http://pdbe.org/6b4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b4m RCSB], [http://www.ebi.ac.uk/pdbsum/6b4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4m ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monotreme lactation protein (MLP) is a recently identified protein with antimicrobial activity. It is present in the milk of monotremes and is unique to this lineage. To characterize MLP and to gain insight into the potential role of this protein in the evolution of lactation, the crystal structure of duck-billed platypus (Ornithorhynchus anatinus) MLP was determined at 1.82 A resolution. This is the first structure to be reported for this novel, mammalian antibacterial protein. MLP was expressed as a FLAG epitope-tagged protein in mammalian cells and crystallized readily, with at least three space groups being observed (P1, C2 and P21). A 1.82 A resolution native data set was collected from a crystal in space group P1, with unit-cell parameters a = 51.2, b = 59.7, c = 63.1 A, alpha = 80.15, beta = 82.98, gamma = 89.27 degrees . The structure was solved by SAD phasing using a protein crystal derivatized with mercury in space group C2, with unit-cell parameters a = 92.7, b = 73.2, c = 56.5 A, beta = 90.28 degrees . MLP comprises a monomer of 12 helices and two short beta-strands, with much of the N-terminus composed of loop regions. The crystal structure of MLP reveals no three-dimensional similarity to any known structures and reveals a heretofore unseen fold, supporting the idea that monotremes may be a rich source for the identification of novel proteins. It is hypothesized that MLP in monotreme milk has evolved to specifically support the unusual lactation strategy of this lineage and may have played a central role in the evolution of these mammals.
		+
		+	Structural characterization of a novel monotreme-specific protein with antimicrobial activity from the milk of the platypus.,Newman J, Sharp JA, Enjapoori AK, Bentley J, Nicholas KR, Adams TE, Peat TS Acta Crystallogr F Struct Biol Commun. 2018 Jan 1;74(Pt 1):39-45. doi:, 10.1107/S2053230X17017708. Epub 2018 Jan 1. PMID:29372906<ref>PMID:29372906</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b4m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Duckbill platypus]]
	[[Category: Adams, T E]]		[[Category: Adams, T E]]
	[[Category: Kumar, A]]		[[Category: Kumar, A]]

OCA at 06:59, 17 January 2018

2018-01-17T06:59:45Z

←Older revision		Revision as of 06:59, 17 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6b4m ~~is ON HOLD until Paper Publication~~	+	==Structural characterization of a novel monotreme-specific protein from the milk of the platypus==
-		+	<StructureSection load='6b4m' size='340' side='right' caption='[[6b4m]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~Authors~~: ~~Peat, T~~.S., ~~Newman~~, J., ~~Sharp, J~~.A., ~~Kumar, A~~., ~~Nicholas, K~~.R., ~~Adams~~, T.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b4m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B4M FirstGlance]. <br>
-	~~Description: Structural characterization of a novel monotreme-specific protein from the milk of the platypus~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~[[Category: Unreleased Structures]]~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4v00\|4v00]], [[4v3j\|4v3j]]</td></tr>
-	[[Category: ~~Sharp~~, ~~J.A~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4m OCA], [http://pdbe.org/6b4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b4m RCSB], [http://www.ebi.ac.uk/pdbsum/6b4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4m ProSAT]</span></td></tr>
-	[[Category: ~~Peat~~, ~~T.S~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adams, T E]]
		+	[[Category: Kumar, A]]
	[[Category: Newman, J]]		[[Category: Newman, J]]
-	[[Category: Nicholas, K.R]]	+	[[Category: Nicholas, K R]]
-	[[Category: ~~Kumar~~, A]]	+	[[Category: Peat, T S]]
-	[[Category: ~~Adams~~, ~~T.E~~]]	+	[[Category: Sharp, J A]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: Monotreme]]
		+	[[Category: Novel-fold]]

OCA: Protected "6b4m" [edit=sysop:move=sysop]

2017-10-04T08:13:45Z

Protected "6b4m" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:13, 4 October 2017

OCA: New page: '''Unreleased structure''' The entry 6b4m is ON HOLD until Paper Publication Authors: Peat, T.S., Newman, J., Sharp, J.A., Kumar, A., Nicholas, K.R., Adams, T.E. Description: Structura...

2017-10-04T08:13:44Z

New page: '''Unreleased structure''' The entry 6b4m is ON HOLD until Paper Publication Authors: Peat, T.S., Newman, J., Sharp, J.A., Kumar, A., Nicholas, K.R., Adams, T.E. Description: Structura...

New page

'''Unreleased structure'''

The entry 6b4m is ON HOLD until Paper Publication

Authors: Peat, T.S., Newman, J., Sharp, J.A., Kumar, A., Nicholas, K.R., Adams, T.E.

Description: Structural characterization of a novel monotreme-specific protein from the milk of the platypus
[[Category: Unreleased Structures]]
[[Category: Sharp, J.A]]
[[Category: Peat, T.S]]
[[Category: Newman, J]]
[[Category: Nicholas, K.R]]
[[Category: Kumar, A]]
[[Category: Adams, T.E]]