6b6t - Revision history

OCA at 10:14, 1 January 2020

2020-01-01T10:14:18Z

←Older revision		Revision as of 10:14, 1 January 2020
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	==Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant==		==Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant==
-	<StructureSection load='6b6t' size='340' side='right' caption='[[6b6t]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6b6t' size='340' side='right'caption='[[6b6t]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6b6t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6T FirstGlance]. <br>		<table><tr><td colspan='2'>[[6b6t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6T FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Juers, D H]]		[[Category: Juers, D H]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:46, 21 February 2019

2019-02-21T07:46:41Z

←Older revision		Revision as of 07:46, 21 February 2019
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	</div>		</div>
	<div class="pdbe-citations 6b6t" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6b6t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:15, 19 September 2018

2018-09-19T20:15:59Z

←Older revision		Revision as of 20:15, 19 September 2018
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	<StructureSection load='6b6t' size='340' side='right' caption='[[6b6t]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6b6t' size='340' side='right' caption='[[6b6t]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b6t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b6t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6t OCA], [http://pdbe.org/6b6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6t RCSB], [http://www.ebi.ac.uk/pdbsum/6b6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6t OCA], [http://pdbe.org/6b6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6t RCSB], [http://www.ebi.ac.uk/pdbsum/6b6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cryocooling of macromolecular crystals is commonly employed to limit radiation damage during X-ray diffraction data collection. However, cooling itself affects macromolecular conformation and often damages crystals via poorly understood processes. Here, the effects of cryosolution thermal contraction on macromolecular conformation and crystal order in crystals ranging from 32 to 67% solvent content are systematically investigated. It is found that the solution thermal contraction affects macromolecule configurations and volumes, unit-cell volumes, crystal packing and crystal order. The effects occur through not only thermal contraction, but also pressure caused by the mismatched contraction of cryosolvent and pores. Higher solvent-content crystals are more affected. In some cases the solvent contraction can be adjusted to reduce mosaicity and increase the strength of diffraction. Ice formation in some crystals is found to cause damage via a reduction in unit-cell volume, which is interpreted through solvent transport out of unit cells during cooling. The results point to more deductive approaches to cryoprotection optimization by adjusting the cryosolution composition to reduce thermal contraction-induced stresses in the crystal with cooling.
		+
		+	The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order.,Juers DH, Farley CA, Saxby CP, Cotter RA, Cahn JKB, Holton-Burke RC, Harrison K, Wu Z Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):922-938. doi:, 10.1107/S2059798318008793. Epub 2018 Sep 5. PMID:30198901<ref>PMID:30198901</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b6t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Juers, D H]]		[[Category: Juers, D H]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:29, 14 March 2018

2018-03-14T06:29:33Z

←Older revision		Revision as of 06:29, 14 March 2018
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-	'''Unreleased structure'''

-	~~The entry 6b6t is ON HOLD~~	+	==Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant==
-		+	<StructureSection load='6b6t' size='340' side='right' caption='[[6b6t]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Juers, D.H.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b6t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6T FirstGlance]. <br>
-	~~Description:~~ Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	[[Category: Juers, D.H]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6t OCA], [http://pdbe.org/6b6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6t RCSB], [http://www.ebi.ac.uk/pdbsum/6b6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6t ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Trypsin]]
		+	[[Category: Juers, D H]]
		+	[[Category: Hydrolase]]

OCA: Protected "6b6t" [edit=sysop:move=sysop]

2017-10-11T05:33:07Z

Protected "6b6t" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:33, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6b6t is ON HOLD Authors: Juers, D.H. Description: Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant [[Category: Unreleas...

2017-10-11T05:33:06Z

New page: '''Unreleased structure''' The entry 6b6t is ON HOLD Authors: Juers, D.H. Description: Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 6b6t is ON HOLD

Authors: Juers, D.H.

Description: Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant
[[Category: Unreleased Structures]]
[[Category: Juers, D.H]]