6b86 - Revision history

OCA at 14:35, 4 October 2023

2023-10-04T14:35:19Z

←Older revision		Revision as of 14:35, 4 October 2023
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	<StructureSection load='6b86' size='340' side='right'caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6b86' size='340' side='right'caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Neucr Neucr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B86 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa_OR74A Neurospora crassa OR74A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B86 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CO:COBALT+(II)+ION'>CO</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~cao-1, NCU07008 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=367110 NEUCR]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b86 OCA], [~~http~~://pdbe.org/6b86 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b86 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b86 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b86 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b86 OCA], [https://pdbe.org/6b86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b86 RCSB], [https://www.ebi.ac.uk/pdbsum/6b86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b86 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CAO1_NEUCR CAO1_NEUCR] Dioxygenase that cleaves the interphenyl C-alpha-C-beta double bond of resveratrol to yield 3,5-dihydroxybenzaldehyde and 4-hydroxybenzaldehyde (PubMed:23893079, PubMed:28493664). Cleaves also piceatannol, a compound that differs from resveratrol only in the occurrence of an additional hydroxyl group, which leads to the production of 3,4-dihydroxybenzaldehyde and 3,5-hydroxybenzaldehyde (PubMed:23893079 PubMed:28493664). Is not able to cleave trans-stilbene, 4-monohydroxy-trans-stilbene, 3,5-dihydroxy-trans-stilbene (pinosylvin), trismethoxy-resveratrol, and 3,3',5-trihydroxy-4'-methoxystilbene-3-O-beta-D-glucoside (PubMed:23893079). Is not involved in carotenoid metabolism (PubMed:23893079).<ref>PMID:23893079</ref> <ref>PMID:28493664</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Neucr~~]]	+	[[Category: Neurospora crassa OR74A]]
-	[[Category: Hill~~, H E~~]]	+	[[Category: Hill HE]]
-	[[Category: Kiser~~, P D]]~~	+	[[Category: Kiser PD]]
-	~~[[Category: Beta-propeller]]~~	+
-	~~[[Category: Dioxygenase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 17:44, 11 December 2019

2019-12-11T17:44:06Z

←Older revision		Revision as of 17:44, 11 December 2019
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	==2.2A Crystal Structure of Co-CAO1==		==2.2A Crystal Structure of Co-CAO1==
-	<StructureSection load='6b86' size='340' side='right' caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='6b86' size='340' side='right'caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Neucr Neucr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B86 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Neucr Neucr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B86 FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Neucr]]		[[Category: Neucr]]
	[[Category: Hill, H E]]		[[Category: Hill, H E]]

OCA at 12:28, 16 January 2019

2019-01-16T12:28:51Z

←Older revision		Revision as of 12:28, 16 January 2019
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	<StructureSection load='6b86' size='340' side='right' caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6b86' size='340' side='right' caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B86 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Neucr Neucr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B86 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cao-1, NCU07008 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367110 NEUCR])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b86 OCA], [http://pdbe.org/6b86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b86 RCSB], [http://www.ebi.ac.uk/pdbsum/6b86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b86 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b86 OCA], [http://pdbe.org/6b86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b86 RCSB], [http://www.ebi.ac.uk/pdbsum/6b86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b86 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carotenoid cleavage oxygenases (CCO) are non-heme iron enzymes that catalyze oxidative cleavage of alkene bonds in carotenoid and stilbenoid substrates. Previously, we showed that the iron cofactor of CAO1, a resveratrol-cleaving member of this family, can be substituted with cobalt to yield a catalytically inert enzyme useful for trapping active site-bound stilbenoid substrates for structural characterization. Metal substitution may provide a general method for identifying the natural substrates for CCOs in addition to facilitating structural and biophysical characterization of CCO-carotenoid complexes under normal aerobic conditions. Here, we demonstrate the general applicability of cobalt substitution in a prototypical carotenoid cleaving CCO, apocarotenoid oxygenase (ACO) from Synechocystis. Among the non-native divalent metals investigated, cobalt was uniquely able to stably occupy the ACO metal binding site and inhibit catalysis. Analysis by X-ray crystallography and X-ray absorption spectroscopy demonstrate that the Co(II) forms of both ACO and CAO1 exhibit a close structural correspondence to the native Fe(II) enzyme forms. Hence, cobalt substitution is an effective strategy for generating catalytically inert but structurally intact forms of CCOs.
		+
		+	Preparation and characterization of metal-substituted carotenoid cleavage oxygenases.,Sui X, Farquhar ER, Hill HE, von Lintig J, Shi W, Kiser PD J Biol Inorg Chem. 2018 Aug;23(6):887-901. doi: 10.1007/s00775-018-1586-0. Epub, 2018 Jun 26. PMID:29946976<ref>PMID:29946976</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b86" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Neucr]]
	[[Category: Hill, H E]]		[[Category: Hill, H E]]
	[[Category: Kiser, P D]]		[[Category: Kiser, P D]]

OCA at 07:10, 4 July 2018

2018-07-04T07:10:51Z

←Older revision		Revision as of 07:10, 4 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6b86 is ~~ON HOLD until Paper Publication~~	+	==2.2A Crystal Structure of Co-CAO1==
-		+	<StructureSection load='6b86' size='340' side='right' caption='[[6b86]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b86]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B86 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b86 OCA], [http://pdbe.org/6b86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b86 RCSB], [http://www.ebi.ac.uk/pdbsum/6b86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b86 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hill, H E]]
		+	[[Category: Kiser, P D]]
		+	[[Category: Beta-propeller]]
		+	[[Category: Dioxygenase]]
		+	[[Category: Oxidoreductase]]

OCA at 12:23, 11 April 2018

2018-04-11T12:23:01Z

←Older revision		Revision as of 12:23, 11 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b86 is ON HOLD	+	The entry 6b86 is ON HOLD until Paper Publication

-	Authors: ~~Hill, H.E., Kiser, P.D.~~	+	Authors:

-	Description: ~~2.2A Crystal Structure of Co-CAO1~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Kiser, P.D]]
-	[[Category: Hill, H.E]]

OCA: Protected "6b86" [edit=sysop:move=sysop]

2017-10-11T05:40:00Z

Protected "6b86" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:40, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6b86 is ON HOLD Authors: Hill, H.E., Kiser, P.D. Description: 2.2A Crystal Structure of Co-CAO1 Category: Unreleased Structures [[Category: Kis...

2017-10-11T05:39:59Z

New page: '''Unreleased structure''' The entry 6b86 is ON HOLD Authors: Hill, H.E., Kiser, P.D. Description: 2.2A Crystal Structure of Co-CAO1 Category: Unreleased Structures [[Category: Kis...

New page

'''Unreleased structure'''

The entry 6b86 is ON HOLD

Authors: Hill, H.E., Kiser, P.D.

Description: 2.2A Crystal Structure of Co-CAO1
[[Category: Unreleased Structures]]
[[Category: Kiser, P.D]]
[[Category: Hill, H.E]]