6bm0 - Revision history

OCA at 14:22, 13 March 2024

2024-03-13T14:22:34Z

←Older revision		Revision as of 14:22, 13 March 2024
Line 3:		Line 3:
	<SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CPSF1~~, ~~CPSF160 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN~~]~~), WDR33, WDC146 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [~~http~~://pdbe.org/6bm0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [https://pdbe.org/6bm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [https://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref~~> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref>~~	+	[https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Nearly all eukaryotic messenger RNA precursors must undergo cleavage and polyadenylation at their 3'-end for maturation. A crucial step in this process is the recognition of the AAUAAA polyadenylation signal (PAS), and the molecular mechanism of this recognition has been a long-standing problem. Here, we report the cryo-electron microscopy structure of a quaternary complex of human CPSF-160, WDR33, CPSF-30, and an AAUAAA RNA at 3.4-A resolution. Strikingly, the AAUAAA PAS assumes an unusual conformation that allows this short motif to be bound directly by both CPSF-30 and WDR33. The A1 and A2 bases are recognized specifically by zinc finger 2 (ZF2) of CPSF-30 and the A4 and A5 bases by ZF3. Interestingly, the U3 and A6 bases form an intramolecular Hoogsteen base pair and directly contact WDR33. CPSF-160 functions as an essential scaffold and preorganizes CPSF-30 and WDR33 for high-affinity binding to AAUAAA. Our findings provide an elegant molecular explanation for how PAS sequences are recognized for mRNA 3'-end formation.	+
-		+
-	Molecular basis for the recognition of the human AAUAAA polyadenylation signal.,Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L Proc Natl Acad Sci U S A. 2017 Dec 5. pii: 1718723115. doi:, 10.1073/pnas.1718723115. PMID:29208711<ref>PMID:29208711</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6bm0" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hamilton, K]]	+	[[Category: Hamilton K]]
-	[[Category: Sun, Y]]	+	[[Category: Sun Y]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	[[Category: Walz, T]]	+	[[Category: Walz T]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang Y]]
-	~~[[Category: Polyadenylation]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Scaffolding protein]]~~	+
-	~~[[Category: Wd40~~]]	+

OCA at 23:24, 10 April 2020

2020-04-10T23:24:20Z

←Older revision		Revision as of 23:24, 10 April 2020
Line 3:		Line 3:
	<SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 20:32, 6 March 2020

2020-03-06T20:32:25Z

←Older revision		Revision as of 20:32, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==		==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==
-	<~~StructureSection~~ load='6bm0' size='340' side='right'caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
Line 21:		Line 21:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 10:22, 1 January 2020

2020-01-01T10:22:33Z

←Older revision		Revision as of 10:22, 1 January 2020
Line 1:		Line 1:

	==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==		==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==
-	<StructureSection load='6bm0' size='340' side='right' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<StructureSection load='6bm0' size='340' side='right'caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hamilton, K]]		[[Category: Hamilton, K]]
	[[Category: Sun, Y]]		[[Category: Sun, Y]]

OCA at 06:46, 20 December 2017

2017-12-20T06:46:31Z

←Older revision		Revision as of 06:46, 20 December 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref>		[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nearly all eukaryotic messenger RNA precursors must undergo cleavage and polyadenylation at their 3'-end for maturation. A crucial step in this process is the recognition of the AAUAAA polyadenylation signal (PAS), and the molecular mechanism of this recognition has been a long-standing problem. Here, we report the cryo-electron microscopy structure of a quaternary complex of human CPSF-160, WDR33, CPSF-30, and an AAUAAA RNA at 3.4-A resolution. Strikingly, the AAUAAA PAS assumes an unusual conformation that allows this short motif to be bound directly by both CPSF-30 and WDR33. The A1 and A2 bases are recognized specifically by zinc finger 2 (ZF2) of CPSF-30 and the A4 and A5 bases by ZF3. Interestingly, the U3 and A6 bases form an intramolecular Hoogsteen base pair and directly contact WDR33. CPSF-160 functions as an essential scaffold and preorganizes CPSF-30 and WDR33 for high-affinity binding to AAUAAA. Our findings provide an elegant molecular explanation for how PAS sequences are recognized for mRNA 3'-end formation.
		+
		+	Molecular basis for the recognition of the human AAUAAA polyadenylation signal.,Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L Proc Natl Acad Sci U S A. 2017 Dec 5. pii: 1718723115. doi:, 10.1073/pnas.1718723115. PMID:29208711<ref>PMID:29208711</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bm0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:02, 6 December 2017

2017-12-06T08:02:32Z

←Older revision		Revision as of 08:02, 6 December 2017
Line 3:		Line 3:
	<StructureSection load='6bm0' size='340' side='right' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='6bm0' size='340' side='right' caption='[[6bm0]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Hamilton, K]]		[[Category: Hamilton, K]]
	[[Category: Sun, Y]]		[[Category: Sun, Y]]

OCA: Protected "6bm0" [edit=sysop:move=sysop]

2017-11-22T07:49:22Z

Protected "6bm0" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:49, 22 November 2017

OCA: New page: ==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==
2017-11-22T07:49:19Z

New page: ==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution== <StructureSection load='6bm0' size='340' side='right' caption='6bm0, resolution 3.80Å' s...

New page

==Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution==
<StructureSection load='6bm0' size='340' side='right' caption='[[6bm0]], [[Resolution|resolution]] 3.80Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Hamilton, K]]
[[Category: Sun, Y]]
[[Category: Tong, L]]
[[Category: Walz, T]]
[[Category: Zhang, Y]]
[[Category: Polyadenylation]]
[[Category: Protein binding]]
[[Category: Scaffolding protein]]
[[Category: Wd40]]