6by2 - Revision history

OCA at 10:30, 1 January 2020

2020-01-01T10:30:11Z

←Older revision		Revision as of 10:30, 1 January 2020
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	==Closed and deep-inactivated conformation of KcsA-T75A mutant==		==Closed and deep-inactivated conformation of KcsA-T75A mutant==
-	<StructureSection load='6by2' size='340' side='right' caption='[[6by2]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='6by2' size='340' side='right'caption='[[6by2]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BY2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BY2 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6by2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6by2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Strco]]		[[Category: Strco]]

OCA at 07:44, 23 May 2018

2018-05-23T07:44:11Z

←Older revision		Revision as of 07:44, 23 May 2018
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	<StructureSection load='6by2' size='340' side='right' caption='[[6by2]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='6by2' size='340' side='right' caption='[[6by2]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BY2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BY2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">kcsA, skc1, SCO7660, SC10F4.33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6by2 OCA], [http://pdbe.org/6by2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6by2 RCSB], [http://www.ebi.ac.uk/pdbsum/6by2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6by2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6by2 OCA], [http://pdbe.org/6by2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6by2 RCSB], [http://www.ebi.ac.uk/pdbsum/6by2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6by2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KCSA_STRCO KCSA_STRCO]] Acts as a potassium ion channel (By similarity).		[[http://www.uniprot.org/uniprot/KCSA_STRCO KCSA_STRCO]] Acts as a potassium ion channel (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The selectivity filter and the activation gate in potassium channels are functionally and structurally coupled. An allosteric coupling underlies C-type inactivation coupled to activation gating in this ion-channel family (i.e., opening of the activation gate triggers the collapse of the channel's selectivity filter). We have identified the second Threonine residue within the TTVGYGD signature sequence of K(+) channels as a crucial residue for this allosteric communication. A Threonine to Alanine substitution at this position was studied in three representative members of the K(+)-channel family. Interestingly, all of the mutant channels exhibited lack of C-type inactivation gating and an inversion of their allosteric coupling (i.e., closing of the activation gate collapses the channel's selectivity filter). A state-dependent crystallographic study of KcsA-T75A proves that, on activation, the selectivity filter transitions from a nonconductive and deep C-type inactivated conformation to a conductive one. Finally, we provide a crystallographic demonstration that closed-state inactivation can be achieved by the structural collapse of the channel's selectivity filter.
		+
		+	Inverted allosteric coupling between activation and inactivation gates in K(+) channels.,Labro AJ, Cortes DM, Tilegenova C, Cuello LG Proc Natl Acad Sci U S A. 2018 May 7. pii: 1800559115. doi:, 10.1073/pnas.1800559115. PMID:29735651<ref>PMID:29735651</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6by2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lk3 transgenic mice]]
		+	[[Category: Strco]]
	[[Category: Cortes, D M]]		[[Category: Cortes, D M]]
	[[Category: Cuello, L G]]		[[Category: Cuello, L G]]

OCA at 06:16, 9 May 2018

2018-05-09T06:16:45Z

←Older revision		Revision as of 06:16, 9 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6by2 ~~is ON HOLD until Paper Publication~~	+	==Closed and deep-inactivated conformation of KcsA-T75A mutant==
-		+	<StructureSection load='6by2' size='340' side='right' caption='[[6by2]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~: ~~Labro, A~~.J., ~~Cortes~~, D.M., ~~Tilegenova~~, C., ~~Cuello~~, L.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6by2]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BY2 FirstGlance]. <br>
-	~~Description~~: ~~Closed and deep~~-~~inactivated conformation of KcsA-T75A mutant~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6by2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6by2 OCA], [http://pdbe.org/6by2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6by2 RCSB], [http://www.ebi.ac.uk/pdbsum/6by2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6by2 ProSAT]</span></td></tr>
-	[[Category: Cortes, D.M]]	+	</table>
-	[[Category: Cuello, L.G]]	+	== Function ==
-	[[Category: Labro, A.J]]	+	[[http://www.uniprot.org/uniprot/KCSA_STRCO KCSA_STRCO]] Acts as a potassium ion channel (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cortes, D M]]
		+	[[Category: Cuello, L G]]
		+	[[Category: Labro, A J]]
	[[Category: Tilegenova, C]]		[[Category: Tilegenova, C]]
		+	[[Category: C-type inactivation]]
		+	[[Category: Ion channel]]
		+	[[Category: Kcsa]]
		+	[[Category: Membrane protein]]
		+	[[Category: Potassium channel]]

OCA at 17:15, 24 January 2018

2018-01-24T17:15:34Z

←Older revision		Revision as of 17:15, 24 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6by2 is ON HOLD	+	The entry 6by2 is ON HOLD until Paper Publication

	Authors: Labro, A.J., Cortes, D.M., Tilegenova, C., Cuello, L.G.		Authors: Labro, A.J., Cortes, D.M., Tilegenova, C., Cuello, L.G.
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	Description: Closed and deep-inactivated conformation of KcsA-T75A mutant		Description: Closed and deep-inactivated conformation of KcsA-T75A mutant
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Cuello, L.G]]
	[[Category: Cortes, D.M]]		[[Category: Cortes, D.M]]
		+	[[Category: Cuello, L.G]]
	[[Category: Labro, A.J]]		[[Category: Labro, A.J]]
	[[Category: Tilegenova, C]]		[[Category: Tilegenova, C]]

OCA: Protected "6by2" [edit=sysop:move=sysop]

2017-12-27T07:22:27Z

Protected "6by2" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:22, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 6by2 is ON HOLD Authors: Labro, A.J., Cortes, D.M., Tilegenova, C., Cuello, L.G. Description: Closed and deep-inactivated conformation of KcsA-T75A...

2017-12-27T07:22:26Z

New page: '''Unreleased structure''' The entry 6by2 is ON HOLD Authors: Labro, A.J., Cortes, D.M., Tilegenova, C., Cuello, L.G. Description: Closed and deep-inactivated conformation of KcsA-T75A...

New page

'''Unreleased structure'''

The entry 6by2 is ON HOLD

Authors: Labro, A.J., Cortes, D.M., Tilegenova, C., Cuello, L.G.

Description: Closed and deep-inactivated conformation of KcsA-T75A mutant
[[Category: Unreleased Structures]]
[[Category: Cuello, L.G]]
[[Category: Cortes, D.M]]
[[Category: Labro, A.J]]
[[Category: Tilegenova, C]]