6c0h - Revision history

OCA at 20:16, 19 September 2018

2018-09-19T20:16:51Z

←Older revision		Revision as of 20:16, 19 September 2018
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	<StructureSection load='6c0h' size='340' side='right' caption='[[6c0h]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6c0h' size='340' side='right' caption='[[6c0h]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6c0h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6c0h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_cinnamomeus_forma_cinnamomeus"_(sic)_pridham_et_al._1956 "streptomyces cinnamomeus forma cinnamomeus" (sic) pridham et al. 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C0H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH2:(3R)-3-HYDROXY-L-ASPARTIC+ACID'>BH2</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH2:(3R)-3-HYDROXY-L-ASPARTIC+ACID'>BH2</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">durN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53446 "Streptomyces cinnamomeus forma cinnamomeus" (sic) Pridham et al. 1956]), durA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53446 "Streptomyces cinnamomeus forma cinnamomeus" (sic) Pridham et al. 1956])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c0h OCA], [http://pdbe.org/6c0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c0h RCSB], [http://www.ebi.ac.uk/pdbsum/6c0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c0h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c0h OCA], [http://pdbe.org/6c0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c0h RCSB], [http://www.ebi.ac.uk/pdbsum/6c0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c0h ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Duramycin is a heavily post-translationally modified peptide that binds phosphatidylethanolamine. It has been investigated as an antibiotic, an inhibitor of viral entry, a therapeutic for cystic fibrosis, and a tumor and vasculature imaging agent. Duramycin contains a beta-hydroxylated Asp (Hya) and four macrocycles, including an essential lysinoalanine (Lal) cross-link. The mechanism of Lal formation is not known. Here we show that Lal is installed stereospecifically by DurN via addition of Lys19 to a dehydroalanine. The structure of DurN reveals an unusual dimer with a new fold. Surprisingly, in the structure of duramycin bound to DurN, no residues of the enzyme are near the Lal cross-link. Instead, Hya15 of the substrate makes interactions with Lal, suggesting it acts as a base to deprotonate Lys19 during catalysis. Biochemical data suggest that DurN preorganizes the reactive conformation of the substrate, such that the Hya15 of the substrate can serve as the catalytic base for Lal formation.
		+
		+	Substrate-assisted enzymatic formation of lysinoalanine in duramycin.,An L, Cogan DP, Navo CD, Jimenez-Oses G, Nair SK, van der Donk WA Nat Chem Biol. 2018 Oct;14(10):928-933. doi: 10.1038/s41589-018-0122-4. Epub 2018, Sep 3. PMID:30177849<ref>PMID:30177849</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6c0h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:05, 5 September 2018

2018-09-05T10:05:23Z

←Older revision		Revision as of 10:05, 5 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6c0h is ~~ON HOLD until Paper Publication~~	+	==Lysinoalanine synthase, DurN, from duramycin biosynthesis bound to 1-Dha6Ala==
-		+	<StructureSection load='6c0h' size='340' side='right' caption='[[6c0h]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6c0h]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C0H FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BH2:(3R)-3-HYDROXY-L-ASPARTIC+ACID'>BH2</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c0h OCA], [http://pdbe.org/6c0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c0h RCSB], [http://www.ebi.ac.uk/pdbsum/6c0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c0h ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cogan, D P]]
		+	[[Category: Nair, S K]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Lysinoalanine synthase]]
		+	[[Category: Michael addition]]

OCA at 03:58, 25 April 2018

2018-04-25T03:58:24Z

←Older revision		Revision as of 03:58, 25 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6c0h is ON HOLD	+	The entry 6c0h is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6c0h" [edit=sysop:move=sysop]

2018-01-10T07:12:08Z

Protected "6c0h" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:12, 10 January 2018

OCA: New page: '''Unreleased structure''' The entry 6c0h is ON HOLD Authors: Description: Category: Unreleased Structures

2018-01-10T07:12:06Z

New page: '''Unreleased structure''' The entry 6c0h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6c0h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]