6cca - Revision history

OCA at 14:27, 13 March 2024

2024-03-13T14:27:37Z

←Older revision		Revision as of 14:27, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"polyangium_compositum"_thaxter_1904 "polyangium compositum" thaxter 1904~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCA FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~dszA~~, ~~disA (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=56 "Polyangium compositum" Thaxter 1904])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [~~http~~://pdbe.org/6cca PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cca PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [https://pdbe.org/6cca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [https://www.ebi.ac.uk/pdbsum/6cca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q4U447_SORCE Q4U447_SORCE]
-	The methyl substituents in products of trans-acyltransferase assembly lines are usually incorporated by S-adenosyl-methionine (SAM)-dependent methyltransferase (MT) domains. The gem-dimethyl moieties within the polyketide disorazol are installed through the iterative action of an MT in the third module of its assembly line. The 1.75-A-resolution crystal structure of this MT helps elucidate how it catalyzes the addition of two methyl groups. Activity assays of point mutants on beta-ketoacyl chains linked to an acyl carrier protein and N-acetylcysteamine provide additional insights into the roles of active site residues. The replacement of an alanine with a phenylalanine at an apparent gatekeeping position resulted in more monomethylation than dimethylation. MTs may form an interface with ketoreductases (KRs) and even mediate the docking of trans-acyltransferase assembly line polypeptides through this association.	+
-		+
-	Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.,Meinke JL, Mehaffey MR, Wagner DT, Sun N, Zhang Z, Brodbelt JS, Keatinge-Clay AT ACS Chem Biol. 2018 Nov 9. doi: ~~10.1021~~/~~acschembio.8b00733. PMID:30371052<ref>PMID:30371052<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6cca" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Polyangium compositum thaxter 1904]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Keatinge-Clay, A T~~]]	+	[[Category: Sorangium cellulosum]]
-	[[Category: ~~Meinke, J L]]~~	+	[[Category: Keatinge-Clay AT]]
-	~~[[Category: Disorazol]]~~	+	[[Category: Meinke JL]]
-	~~[[Category: Gem~~-~~dimethyl~~]]	+
-	[[Category: ~~Methyltransferase]]~~	+
-	~~[[Category: Polyketide assembly line]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:06, 1 May 2019

2019-05-01T09:06:01Z

←Older revision		Revision as of 09:06, 1 May 2019
Line 1:		Line 1:

	==Crystal structure of DszA carbon methyltransferase==		==Crystal structure of DszA carbon methyltransferase==
-	<StructureSection load='6cca' size='340' side='right' caption='[[6cca]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='6cca' size='340' side='right'caption='[[6cca]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"polyangium_compositum"_thaxter_1904 "polyangium compositum" thaxter 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dszA, disA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=56 "Polyangium compositum" Thaxter 1904])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Polyangium compositum thaxter 1904]]
		+	[[Category: Large Structures]]
	[[Category: Keatinge-Clay, A T]]		[[Category: Keatinge-Clay, A T]]
	[[Category: Meinke, J L]]		[[Category: Meinke, J L]]

OCA at 06:33, 5 December 2018

2018-12-05T06:33:21Z

←Older revision		Revision as of 06:33, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6cca is ~~ON HOLD~~	+	==Crystal structure of DszA carbon methyltransferase==
		+	<StructureSection load='6cca' size='340' side='right' caption='[[6cca]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6cca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCA FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cca OCA], [http://pdbe.org/6cca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cca RCSB], [http://www.ebi.ac.uk/pdbsum/6cca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cca ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The methyl substituents in products of trans-acyltransferase assembly lines are usually incorporated by S-adenosyl-methionine (SAM)-dependent methyltransferase (MT) domains. The gem-dimethyl moieties within the polyketide disorazol are installed through the iterative action of an MT in the third module of its assembly line. The 1.75-A-resolution crystal structure of this MT helps elucidate how it catalyzes the addition of two methyl groups. Activity assays of point mutants on beta-ketoacyl chains linked to an acyl carrier protein and N-acetylcysteamine provide additional insights into the roles of active site residues. The replacement of an alanine with a phenylalanine at an apparent gatekeeping position resulted in more monomethylation than dimethylation. MTs may form an interface with ketoreductases (KRs) and even mediate the docking of trans-acyltransferase assembly line polypeptides through this association.

-	~~Authors~~:	+	Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.,Meinke JL, Mehaffey MR, Wagner DT, Sun N, Zhang Z, Brodbelt JS, Keatinge-Clay AT ACS Chem Biol. 2018 Nov 9. doi: 10.1021/acschembio.8b00733. PMID:30371052<ref>PMID:30371052</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6cca" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Keatinge-Clay, A T]]
		+	[[Category: Meinke, J L]]
		+	[[Category: Disorazol]]
		+	[[Category: Gem-dimethyl]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Polyketide assembly line]]
		+	[[Category: Transferase]]

OCA: Protected "6cca" [edit=sysop:move=sysop]

2018-02-15T05:22:09Z

Protected "6cca" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 15 February 2018

OCA: New page: '''Unreleased structure''' The entry 6cca is ON HOLD Authors: Description: Category: Unreleased Structures

2018-02-15T05:22:08Z

New page: '''Unreleased structure''' The entry 6cca is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6cca is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]