6cj0 - Revision history

OCA at 07:05, 3 April 2024

2024-04-03T07:05:13Z

←Older revision		Revision as of 07:05, 3 April 2024
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	==Chromosomal trehalose-6-phosphate phosphatase from P. aeruginosa==		==Chromosomal trehalose-6-phosphate phosphatase from P. aeruginosa==
-	<StructureSection load='6cj0' size='340' side='right' caption='[[6cj0]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='6cj0' size='340' side='right'caption='[[6cj0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cj0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJ0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CJ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CJ0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CO3:CARBONATE+ION'>CO3</scene~~>, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CAZ10_36670, PACL_0415, PAMH19_6415 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cj0 OCA], [https://pdbe.org/6cj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cj0 RCSB], [https://www.ebi.ac.uk/pdbsum/6cj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cj0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cj0 OCA], [~~http~~://pdbe.org/6cj0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cj0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cj0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cj0 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/B3G2E1_PSEAI B3G2E1_PSEAI]
-	The opportunistic bacterium Pseudomonas aeruginosa has been recognized as an important pathogen of clinical relevance and is a leading cause of hospital-acquired infections. The presence of a glycolytic enzyme in Pseudomonas, which is known to be inhibited by trehalose 6-phosphate (T6P) in other organisms, suggests that these bacteria may be vulnerable to the detrimental effects of intracellular T6P accumulation. In the present study, we explored the structural and functional properties of trehalose 6-phosphate phosphatase (TPP) in P. aeruginosa in support of future target-based drug discovery. A survey of genomes revealed the existence of 2 TPP genes with either chromosomal or extrachromosomal location. Both TPPs were produced as recombinant proteins, and characterization of their enzymatic properties confirmed specific, magnesium-dependent catalytic hydrolysis of T6P. The 3-dimensional crystal structure of the chromosomal TPP revealed a protein dimer arising through beta-sheet expansion of the individual monomers, which possess the overall fold of halo-acid dehydrogenases.-Cross, M., Biberacher, S., Park, S.-Y., Rajan, S., Korhonen, P., Gasser, R. B., Kim, J.-S., Coster, M. J., Hofmann, A. Trehalose 6-phosphate phosphatases of Pseudomonas aeruginosa.	+
-		+
-	~~Trehalose 6-phosphate phosphatases of Pseudomonas aeruginosa.,Cross M, Biberacher S, Park SY, Rajan S, Korhonen P, Gasser RB, Kim JS, Coster MJ, Hofmann A FASEB J. 2018 Apr 24~~:~~fj201800500R. doi: 10.1096~~/~~fj.201800500R. PMID:29688811<ref>PMID:29688811<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6cj0" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cross, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hofmann, A~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Park, S Y~~]]	+	[[Category: Cross M]]
-	[[Category: ~~Had superfamily/rossmann fold~~]]	+	[[Category: Hofmann A]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Park S-Y]]
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Phosphohydrolase]]~~	+
-	~~[[Category: Trehalose~~-~~6-phosphate~~]]	+

OCA at 08:30, 10 October 2018

2018-10-10T08:30:28Z

←Older revision		Revision as of 08:30, 10 October 2018
Line 3:		Line 3:
	<StructureSection load='6cj0' size='340' side='right' caption='[[6cj0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6cj0' size='340' side='right' caption='[[6cj0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cj0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CJ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cj0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CJ0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CAZ10_36670, PACL_0415, PAMH19_6415 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cj0 OCA], [http://pdbe.org/6cj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6cj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cj0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cj0 OCA], [http://pdbe.org/6cj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6cj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cj0 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:18, 9 May 2018

2018-05-09T06:18:27Z

←Older revision		Revision as of 06:18, 9 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6cj0 is ~~ON HOLD until Paper~~ Publication	+	==Chromosomal trehalose-6-phosphate phosphatase from P. aeruginosa==
		+	<StructureSection load='6cj0' size='340' side='right' caption='[[6cj0]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6cj0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CJ0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cj0 OCA], [http://pdbe.org/6cj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6cj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cj0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The opportunistic bacterium Pseudomonas aeruginosa has been recognized as an important pathogen of clinical relevance and is a leading cause of hospital-acquired infections. The presence of a glycolytic enzyme in Pseudomonas, which is known to be inhibited by trehalose 6-phosphate (T6P) in other organisms, suggests that these bacteria may be vulnerable to the detrimental effects of intracellular T6P accumulation. In the present study, we explored the structural and functional properties of trehalose 6-phosphate phosphatase (TPP) in P. aeruginosa in support of future target-based drug discovery. A survey of genomes revealed the existence of 2 TPP genes with either chromosomal or extrachromosomal location. Both TPPs were produced as recombinant proteins, and characterization of their enzymatic properties confirmed specific, magnesium-dependent catalytic hydrolysis of T6P. The 3-dimensional crystal structure of the chromosomal TPP revealed a protein dimer arising through beta-sheet expansion of the individual monomers, which possess the overall fold of halo-acid dehydrogenases.-Cross, M., Biberacher, S., Park, S.-Y., Rajan, S., Korhonen, P., Gasser, R. B., Kim, J.-S., Coster, M. J., Hofmann, A. Trehalose 6-phosphate phosphatases of Pseudomonas aeruginosa.

-	~~Authors~~:	+	Trehalose 6-phosphate phosphatases of Pseudomonas aeruginosa.,Cross M, Biberacher S, Park SY, Rajan S, Korhonen P, Gasser RB, Kim JS, Coster MJ, Hofmann A FASEB J. 2018 Apr 24:fj201800500R. doi: 10.1096/fj.201800500R. PMID:29688811<ref>PMID:29688811</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6cj0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cross, M]]
		+	[[Category: Hofmann, A]]
		+	[[Category: Park, S Y]]
		+	[[Category: Had superfamily/rossmann fold]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lyase]]
		+	[[Category: Phosphohydrolase]]
		+	[[Category: Trehalose-6-phosphate]]

OCA at 06:06, 21 March 2018

2018-03-21T06:06:47Z

←Older revision		Revision as of 06:06, 21 March 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6cj0 is ON HOLD	+	The entry 6cj0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6cj0" [edit=sysop:move=sysop]

2018-03-08T03:47:14Z

Protected "6cj0" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:47, 8 March 2018

OCA: New page: '''Unreleased structure''' The entry 6cj0 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-03-08T03:47:13Z

New page: '''Unreleased structure''' The entry 6cj0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6cj0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]