6cwc - Revision history

OCA at 14:31, 13 March 2024

2024-03-13T14:31:04Z

←Older revision		Revision as of 14:31, 13 March 2024
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	<StructureSection load='6cwc' size='340' side='right'caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6cwc' size='340' side='right'caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_6344 Atcc 6344~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CWC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_alvei Paenibacillus alvei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CWC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6cwf\|6cwf]], [[6cwh\|6cwh]], [[6cwi\|6cwi]], [[6cwl\|6cwl]], [[6cwm\|6cwm]], [[6cwn\|6cwn]], [[6cwr\|6cwr]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">spaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44250 ATCC 6344])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cwc OCA], [https://pdbe.org/6cwc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cwc RCSB], [https://www.ebi.ac.uk/pdbsum/6cwc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cwc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cwc OCA], [~~http~~://pdbe.org/6cwc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cwc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cwc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cwc ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/C1JZ07_PAEAL C1JZ07_PAEAL]
-	Self-assembling protein surface (S-) layers are common cell envelope structures of prokaryotes and have critical roles from structural maintenance to virulence. S-layers of Gram-positive bacteria are often attached through the interaction of S-layer homology (SLH) domain trimers with peptidoglycan-linked secondary cell wall polymers (SCWPs). Here we present an in-depth characterization of this interaction, with co-crystal structures of the three consecutive SLH domains from the Paenibacillus alvei S-layer protein SpaA with defined SCWP ligands. The most highly conserved SLH domain residue SLH-Gly29 is shown to enable a peptide backbone flip essential for SCWP binding in both biophysical and cellular experiments. Furthermore, we find that a significant domain movement mediates binding by two different sites in the SLH domain trimer, which may allow anchoring readjustment to relieve S-layer strain caused by cell growth and division.	+
-		+
-	Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei.,Blackler RJ, Lopez-Guzman A, Hager FF, Janesch B, Martinz G, Gagnon SML, Haji-Ghassemi O, Kosma P, Messner P, Schaffer C, Evans SV Nat Commun. 2018 Aug 7;9(1):~~3120. doi: 10.1038~~/~~s41467-018-05471-3. PMID:30087354<ref>PMID:30087354<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6cwc" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 6344]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Blackler, R J~~]]	+	[[Category: Paenibacillus alvei]]
-	[[Category: ~~Evans, S V~~]]	+	[[Category: Blackler RJ]]
-	[[Category: ~~S-layer]]~~	+	[[Category: Evans SV]]
-	~~[[Category: Slh]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Surface layer homology domain~~]]	+

OCA at 08:47, 8 January 2020

2020-01-08T08:47:18Z

←Older revision		Revision as of 08:47, 8 January 2020
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	==Crystal structure of SpaA-SLH==		==Crystal structure of SpaA-SLH==
-	<StructureSection load='6cwc' size='340' side='right' caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='6cwc' size='340' side='right'caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_6344 Atcc 6344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_6344 Atcc 6344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWC FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 6344]]		[[Category: Atcc 6344]]
		+	[[Category: Large Structures]]
	[[Category: Blackler, R J]]		[[Category: Blackler, R J]]
	[[Category: Evans, S V]]		[[Category: Evans, S V]]

OCA at 06:34, 22 August 2018

2018-08-22T06:34:25Z

←Older revision		Revision as of 06:34, 22 August 2018
Line 3:		Line 3:
	<StructureSection load='6cwc' size='340' side='right' caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6cwc' size='340' side='right' caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_6344 Atcc 6344]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6cwf\|6cwf]], [[6cwh\|6cwh]], [[6cwi\|6cwi]], [[6cwl\|6cwl]], [[6cwm\|6cwm]], [[6cwn\|6cwn]], [[6cwr\|6cwr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6cwf\|6cwf]], [[6cwh\|6cwh]], [[6cwi\|6cwi]], [[6cwl\|6cwl]], [[6cwm\|6cwm]], [[6cwn\|6cwn]], [[6cwr\|6cwr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44250 ATCC 6344])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cwc OCA], [http://pdbe.org/6cwc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cwc RCSB], [http://www.ebi.ac.uk/pdbsum/6cwc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cwc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cwc OCA], [http://pdbe.org/6cwc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cwc RCSB], [http://www.ebi.ac.uk/pdbsum/6cwc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cwc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Self-assembling protein surface (S-) layers are common cell envelope structures of prokaryotes and have critical roles from structural maintenance to virulence. S-layers of Gram-positive bacteria are often attached through the interaction of S-layer homology (SLH) domain trimers with peptidoglycan-linked secondary cell wall polymers (SCWPs). Here we present an in-depth characterization of this interaction, with co-crystal structures of the three consecutive SLH domains from the Paenibacillus alvei S-layer protein SpaA with defined SCWP ligands. The most highly conserved SLH domain residue SLH-Gly29 is shown to enable a peptide backbone flip essential for SCWP binding in both biophysical and cellular experiments. Furthermore, we find that a significant domain movement mediates binding by two different sites in the SLH domain trimer, which may allow anchoring readjustment to relieve S-layer strain caused by cell growth and division.
		+
		+	Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei.,Blackler RJ, Lopez-Guzman A, Hager FF, Janesch B, Martinz G, Gagnon SML, Haji-Ghassemi O, Kosma P, Messner P, Schaffer C, Evans SV Nat Commun. 2018 Aug 7;9(1):3120. doi: 10.1038/s41467-018-05471-3. PMID:30087354<ref>PMID:30087354</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6cwc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 6344]]
	[[Category: Blackler, R J]]		[[Category: Blackler, R J]]
	[[Category: Evans, S V]]		[[Category: Evans, S V]]

OCA at 16:14, 15 August 2018

2018-08-15T16:14:14Z

←Older revision		Revision as of 16:14, 15 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6cwc is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of SpaA-SLH==
-		+	<StructureSection load='6cwc' size='340' side='right' caption='[[6cwc]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6cwc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CWC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CWC FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6cwf\|6cwf]], [[6cwh\|6cwh]], [[6cwi\|6cwi]], [[6cwl\|6cwl]], [[6cwm\|6cwm]], [[6cwn\|6cwn]], [[6cwr\|6cwr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cwc OCA], [http://pdbe.org/6cwc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cwc RCSB], [http://www.ebi.ac.uk/pdbsum/6cwc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cwc ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blackler, R J]]
		+	[[Category: Evans, S V]]
		+	[[Category: S-layer]]
		+	[[Category: Slh]]
		+	[[Category: Sugar binding protein]]
		+	[[Category: Surface layer homology domain]]

OCA at 05:56, 23 May 2018

2018-05-23T05:56:26Z

←Older revision		Revision as of 05:56, 23 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6cwc is ON HOLD	+	The entry 6cwc is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6cwc" [edit=sysop:move=sysop]

2018-04-11T12:28:57Z

Protected "6cwc" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:28, 11 April 2018

OCA: New page: '''Unreleased structure''' The entry 6cwc is ON HOLD Authors: Description: Category: Unreleased Structures

2018-04-11T12:28:55Z

New page: '''Unreleased structure''' The entry 6cwc is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6cwc is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]