6d6h - Revision history

OCA at 10:56, 1 January 2020

2020-01-01T10:56:35Z

←Older revision		Revision as of 10:56, 1 January 2020
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	==Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant==		==Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant==
-	<StructureSection load='6d6h' size='340' side='right' caption='[[6d6h]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6d6h' size='340' side='right'caption='[[6d6h]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6d6h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6H FirstGlance]. <br>		<table><tr><td colspan='2'>[[6d6h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6H FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6d6h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6d6h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Juers, D H]]		[[Category: Juers, D H]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:13, 31 October 2018

2018-10-31T07:13:08Z

←Older revision		Revision as of 07:13, 31 October 2018
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cryocooling of macromolecular crystals is commonly employed to limit radiation damage during X-ray diffraction data collection. However, cooling itself affects macromolecular conformation and often damages crystals via poorly understood processes. Here, the effects of cryosolution thermal contraction on macromolecular conformation and crystal order in crystals ranging from 32 to 67% solvent content are systematically investigated. It is found that the solution thermal contraction affects macromolecule configurations and volumes, unit-cell volumes, crystal packing and crystal order. The effects occur through not only thermal contraction, but also pressure caused by the mismatched contraction of cryosolvent and pores. Higher solvent-content crystals are more affected. In some cases the solvent contraction can be adjusted to reduce mosaicity and increase the strength of diffraction. Ice formation in some crystals is found to cause damage via a reduction in unit-cell volume, which is interpreted through solvent transport out of unit cells during cooling. The results point to more deductive approaches to cryoprotection optimization by adjusting the cryosolution composition to reduce thermal contraction-induced stresses in the crystal with cooling.
		+
		+	The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order.,Juers DH, Farley CA, Saxby CP, Cotter RA, Cahn JKB, Holton-Burke RC, Harrison K, Wu Z Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):922-938. doi:, 10.1107/S2059798318008793. Epub 2018 Sep 5. PMID:30198901<ref>PMID:30198901</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6d6h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:49, 19 September 2018

2018-09-19T19:49:09Z

←Older revision		Revision as of 19:49, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry 6d6h is ON HOLD until Paper Publication~~	+	==Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant==
-		+	<StructureSection load='6d6h' size='340' side='right' caption='[[6d6h]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Juers, D.H.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6d6h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6H FirstGlance]. <br>
-	~~Description:~~ Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	[[Category: Juers, D.H]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6h OCA], [http://pdbe.org/6d6h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6h RCSB], [http://www.ebi.ac.uk/pdbsum/6d6h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6h ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
		+	[[Category: Juers, D H]]
		+	[[Category: Glycosidase]]
		+	[[Category: Hydrolase]]

OCA at 05:46, 25 July 2018

2018-07-25T05:46:54Z

←Older revision		Revision as of 05:46, 25 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6d6h is ON HOLD	+	The entry 6d6h is ON HOLD until Paper Publication

	Authors: Juers, D.H.		Authors: Juers, D.H.

OCA: Protected "6d6h" [edit=sysop:move=sysop]

2018-05-02T06:44:34Z

Protected "6d6h" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 2 May 2018

OCA: New page: '''Unreleased structure''' The entry 6d6h is ON HOLD Authors: Juers, D.H. Description: Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant [[Category: Unreleased Stru...

2018-05-02T06:44:32Z

New page: '''Unreleased structure''' The entry 6d6h is ON HOLD Authors: Juers, D.H. Description: Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 6d6h is ON HOLD

Authors: Juers, D.H.

Description: Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant
[[Category: Unreleased Structures]]
[[Category: Juers, D.H]]