6d6i - Revision history

OCA at 15:19, 4 October 2023

2023-10-04T15:19:16Z

←Older revision		Revision as of 15:19, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='6d6i' size='340' side='right'caption='[[6d6i]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='6d6i' size='340' side='right'caption='[[6d6i]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d6i]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6D6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d6i]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D6I FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>UBE2V1, CROC1, UBE2V, UEV1, P/OKcl.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=9606 HUMAN]), UBE2N, BLU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Uba52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.551Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/E2_ubiquitin~~-~~conjugating_enzyme E2 ubiquitin-conjugating enzyme]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.~~3.2.23 2.3.2.23] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6i OCA], [https://pdbe.org/6d6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d6i RCSB], [https://www.ebi.ac.uk/pdbsum/6d6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6i ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6d6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6i OCA], [~~http~~://pdbe.org/6d6i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6d6i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6d6i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6i ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref> [[http://www.uniprot.org/uniprot/UBE2N_HUMAN UBE2N_HUMAN]] The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).<ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:19269966</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>	+	[https://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6d6i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6d6i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of ubiquitin\|3D structures of ubiquitin]]
		+	*[[3D structures of ubiquitin conjugating enzyme\|3D structures of ubiquitin conjugating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~E2 ubiquitin-conjugating enzyme]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ceccarelli~~, D F~~]]	+	[[Category: Ceccarelli DF]]
-	[[Category: Garg, P]]	+	[[Category: Garg P]]
-	[[Category: Keszei, A]]	+	[[Category: Keszei A]]
-	[[Category: Sicheri, F]]	+	[[Category: Sicheri F]]
-	[[Category: Sidhu, S]]	+	[[Category: Sidhu S]]
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Ube2v1]]~~	+
-	~~[[Category: Ubiquitin]]~~	+
-	~~[[Category: Ubiquitin conjugating enzyme]]~~	+
-	~~[[Category: Ubiquitin variant~~]]	+

OCA at 07:46, 6 November 2019

2019-11-06T07:46:03Z

←Older revision		Revision as of 07:46, 6 November 2019
Line 3:		Line 3:
	<StructureSection load='6d6i' size='340' side='right'caption='[[6d6i]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='6d6i' size='340' side='right'caption='[[6d6i]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d6i]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d6i]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6I FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/E2_ubiquitin-conjugating_enzyme E2 ubiquitin-conjugating enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.23 2.3.2.23] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1, P/OKcl.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), UBE2N, BLU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Uba52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/E2_ubiquitin-conjugating_enzyme E2 ubiquitin-conjugating enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.23 2.3.2.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6i OCA], [http://pdbe.org/6d6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6i RCSB], [http://www.ebi.ac.uk/pdbsum/6d6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6i OCA], [http://pdbe.org/6d6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6i RCSB], [http://www.ebi.ac.uk/pdbsum/6d6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref> [[http://www.uniprot.org/uniprot/UBE2N_HUMAN UBE2N_HUMAN]] The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).<ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:19269966</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>		[[http://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref> [[http://www.uniprot.org/uniprot/UBE2N_HUMAN UBE2N_HUMAN]] The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).<ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:19269966</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ubiquitin-conjugating E2 enzymes are central to the ubiquitination cascade and have been implicated in cancer and other diseases. Despite strong interest in developing specific E2 inhibitors, the shallow and exposed active site has proven recalcitrant to targeting with reversible small-molecule inhibitors. Here, we used phage display to generate highly potent and selective ubiquitin variants (UbVs) that target the E2 backside, which is located opposite to the active site. A UbV targeting Ube2D1 did not affect charging but greatly attenuated chain elongation. Likewise, a UbV targeting the E2 variant Ube2V1 did not interfere with the charging of its partner E2 enzyme but inhibited formation of di-ubiquitin. In contrast, a UbV that bound to the backside of Ube2G1 impeded the generation of thioester-linked ubiquitin to the active site cysteine of Ube2G1 by the E1 enzyme. Crystal structures of UbVs in complex with three E2 proteins revealed distinctive molecular interactions in each case, but they also highlighted a common backside pocket that the UbVs utilized for enhanced affinity and specificity. These findings validate the E2 backside as a target for inhibition and provide structural insights to aid inhibitor design and screening efforts.
		+
		+	Structural and Functional Analysis of Ubiquitin-based inhibitors that Target the Backsides of E2 Enzymes.,Garg P, Ceccarelli DF, Keszei AF, Kourinov I, Sicheri F, Sidhu SS J Mol Biol. 2019 Oct 18. pii: S0022-2836(18)30280-8. doi:, 10.1016/j.jmb.2019.09.024. PMID:31634471<ref>PMID:31634471</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6d6i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 14:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: E2 ubiquitin-conjugating enzyme]]		[[Category: E2 ubiquitin-conjugating enzyme]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ceccarelli, D F]]		[[Category: Ceccarelli, D F]]

OCA at 10:36, 17 July 2019

2019-07-17T10:36:30Z

←Older revision		Revision as of 10:36, 17 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6d6i is ~~ON HOLD until Paper Publication~~	+	==Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13==
-		+	<StructureSection load='6d6i' size='340' side='right'caption='[[6d6i]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~Authors~~: ~~Ceccarelli~~, D.F.~~, Garg, P~~., ~~Keszei~~, A.~~, Sidhu, S~~., ~~Sicheri~~, F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6d6i]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D6I FirstGlance]. <br>
-	~~Description~~: ~~Ube2V1~~ in complex with ~~ubiquitin variant Ubv~~.V1.1 and ~~Ube2N~~/~~Ubc13~~	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/E2_ubiquitin-conjugating_enzyme E2 ubiquitin-conjugating enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.23 2.3.2.23] </span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d6i OCA], [http://pdbe.org/6d6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d6i RCSB], [http://www.ebi.ac.uk/pdbsum/6d6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d6i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref> [[http://www.uniprot.org/uniprot/UBE2N_HUMAN UBE2N_HUMAN]] The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).<ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:19269966</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: E2 ubiquitin-conjugating enzyme]]
		+	[[Category: Large Structures]]
		+	[[Category: Ceccarelli, D F]]
		+	[[Category: Garg, P]]
	[[Category: Keszei, A]]		[[Category: Keszei, A]]
-	[[Category: Garg, P]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]
	[[Category: Sidhu, S]]		[[Category: Sidhu, S]]
-	[[Category: ~~Ceccarelli, D.F~~]]	+	[[Category: Transferase]]
		+	[[Category: Ube2v1]]
		+	[[Category: Ubiquitin]]
		+	[[Category: Ubiquitin conjugating enzyme]]
		+	[[Category: Ubiquitin variant]]

OCA at 10:18, 16 January 2019

2019-01-16T10:18:11Z

←Older revision		Revision as of 10:18, 16 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6d6i is ON HOLD	+	The entry 6d6i is ON HOLD until Paper Publication

	Authors: Ceccarelli, D.F., Garg, P., Keszei, A., Sidhu, S., Sicheri, F.		Authors: Ceccarelli, D.F., Garg, P., Keszei, A., Sidhu, S., Sicheri, F.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Keszei, A]]		[[Category: Keszei, A]]
		+	[[Category: Garg, P]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]
	[[Category: Sidhu, S]]		[[Category: Sidhu, S]]
	[[Category: Ceccarelli, D.F]]		[[Category: Ceccarelli, D.F]]
-	[[Category: Garg, P]]

OCA at 06:06, 26 September 2018

2018-09-26T06:06:01Z

←Older revision		Revision as of 06:06, 26 September 2018
Line 3:		Line 3:
	The entry 6d6i is ON HOLD		The entry 6d6i is ON HOLD

-	Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.	+	Authors: Ceccarelli, D.F., Garg, P., Keszei, A., Sidhu, S., Sicheri, F.

	Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13		Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Keszei, A]]
	[[Category: Sicheri, F]]		[[Category: Sicheri, F]]
	[[Category: Sidhu, S]]		[[Category: Sidhu, S]]
	[[Category: Ceccarelli, D.F]]		[[Category: Ceccarelli, D.F]]
	[[Category: Garg, P]]		[[Category: Garg, P]]

OCA at 18:04, 19 September 2018

2018-09-19T18:04:01Z

←Older revision		Revision as of 18:04, 19 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6d6i is ON HOLD ~~until sometime in the future~~	+	The entry 6d6i is ON HOLD

	Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.		Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.

OCA at 04:01, 16 May 2018

2018-05-16T04:01:17Z

←Older revision		Revision as of 04:01, 16 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6d6i is ON HOLD	+	The entry 6d6i is ON HOLD until sometime in the future

	Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.		Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.
Line 7:		Line 7:
	Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13		Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Sicheri, F]]
	[[Category: Sidhu, S]]		[[Category: Sidhu, S]]
	[[Category: Ceccarelli, D.F]]		[[Category: Ceccarelli, D.F]]
-	[[Category: Sicheri, F]]
	[[Category: Garg, P]]		[[Category: Garg, P]]

OCA: Protected "6d6i" [edit=sysop:move=sysop]

2018-05-02T06:44:37Z

Protected "6d6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 2 May 2018

OCA: New page: '''Unreleased structure''' The entry 6d6i is ON HOLD Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F. Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2...

2018-05-02T06:44:35Z

New page: '''Unreleased structure''' The entry 6d6i is ON HOLD Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F. Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2...

New page

'''Unreleased structure'''

The entry 6d6i is ON HOLD

Authors: Ceccarelli, D.F., Garg, P., Sidhu, S., Sicheri, F.

Description: Ube2V1 in complex with ubiquitin variant Ubv.V1.1 and Ube2N/Ubc13
[[Category: Unreleased Structures]]
[[Category: Sidhu, S]]
[[Category: Ceccarelli, D.F]]
[[Category: Sicheri, F]]
[[Category: Garg, P]]