6dba - Revision history

OCA at 15:21, 4 October 2023

2023-10-04T15:21:17Z

←Older revision		Revision as of 15:21, 4 October 2023
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	<StructureSection load='6dba' size='340' side='right'caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='6dba' size='340' side='right'caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arabian_camel Arabian camel~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6DBA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DBA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dba OCA], [~~http~~://pdbe.org/6dba PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6dba RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6dba PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6dba ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dba OCA], [https://pdbe.org/6dba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dba RCSB], [https://www.ebi.ac.uk/pdbsum/6dba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dba ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabian camel~~]]	+	[[Category: Camelus dromedarius]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brooks~~, C L~~]]	+	[[Category: Brooks CL]]
-	[[Category: Huh, I]]	+	[[Category: Huh I]]
-	[[Category: King, M ~~Toride]]~~	+	[[Category: Toride King M]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Nanobody vhh listeria~~]]	+

OCA at 07:46, 6 November 2019

2019-11-06T07:46:05Z

←Older revision		Revision as of 07:46, 6 November 2019
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	==Crystal Structure of VHH R303==		==Crystal Structure of VHH R303==
-	<StructureSection load='6dba' size='340' side='right' caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='6dba' size='340' side='right'caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBA FirstGlance]. <br>		<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBA FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Arabian camel]]		[[Category: Arabian camel]]
		+	[[Category: Large Structures]]
	[[Category: Brooks, C L]]		[[Category: Brooks, C L]]
	[[Category: Huh, I]]		[[Category: Huh, I]]

OCA at 07:36, 25 July 2018

2018-07-25T07:36:16Z

←Older revision		Revision as of 07:36, 25 July 2018
Line 3:		Line 3:
	<StructureSection load='6dba' size='340' side='right' caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='6dba' size='340' side='right' caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dba OCA], [http://pdbe.org/6dba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dba RCSB], [http://www.ebi.ac.uk/pdbsum/6dba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dba ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dba OCA], [http://pdbe.org/6dba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dba RCSB], [http://www.ebi.ac.uk/pdbsum/6dba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dba ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Listeria monocytogenes causes Listeriosis, a potentially fatal food borne disease. The condition is especially harmful to pregnant women. Listeria outbreaks can originate from diverse foods, highlighting the need for novel strategies to improve food safety. The first step in Listeria invasion is internalization of the bacteria, which is mediated by the interaction of the internalin family of virulence factors with host cell receptors. A crucial interaction for Listeria invasion of the placenta, and thus is a target for therapeutic intervention, is between Internalin B (InlB) and the receptor c-Met.. Single-domain antibodies (VHH, also called nanobodies, or sdAbs) from camel heavy-chain antibodies are a novel solution for preventing Listeria infections. The VHH R303, R330 and R326 all bind InlB with high affinity, however the molecular mechanism behind their mode of action was unknown. We demonstrate that despite a high degree of sequence and structural diversity, the VHH bind a single epitope on InlB. A combination of gentamicin protection assays and florescent microscopy establish that InlB specific VHH inhibit Listeria invasion of HeLa cells. A high resolution X-ray structure of VHH R303 in complex with InlB showed that the VHH binds at the c-Met interaction site on InlB, thereby acting as a competitive inhibitor preventing bacterial invasion. These results point to the potential of VHH as a novel class of therapeutics for the prevention of Listeriosis.
		+
		+	Structural Basis of VHH mediated neutralization of the foodborne pathogen Listeria monocytogenes.,Toride King M, Huh I, Shenai A, Brooks TM, Brooks CL J Biol Chem. 2018 Jul 5. pii: RA118.003888. doi: 10.1074/jbc.RA118.003888. PMID:29976754<ref>PMID:29976754</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6dba" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabian camel]]
	[[Category: Brooks, C L]]		[[Category: Brooks, C L]]
	[[Category: Huh, I]]		[[Category: Huh, I]]

OCA at 07:38, 18 July 2018

2018-07-18T07:38:04Z

←Older revision		Revision as of 07:38, 18 July 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6dba ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of VHH R303==
-		+	<StructureSection load='6dba' size='340' side='right' caption='[[6dba]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~Authors~~: ~~Brooks~~, C.L., ~~Toride King~~, M., ~~Huh~~, I.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6dba]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DBA FirstGlance]. <br>
-	~~Description: Crystal Structure of VHH R303~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dba OCA], [http://pdbe.org/6dba PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dba RCSB], [http://www.ebi.ac.uk/pdbsum/6dba PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dba ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brooks, C L]]
	[[Category: Huh, I]]		[[Category: Huh, I]]
-	[[Category: ~~Brooks~~, ~~C.L~~]]	+	[[Category: King, M Toride]]
-	[[Category: ~~Toride King, M~~]]	+	[[Category: Immune system]]
		+	[[Category: Nanobody vhh listeria]]

OCA at 04:03, 16 May 2018

2018-05-16T04:03:46Z

←Older revision		Revision as of 04:03, 16 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dba is ON HOLD	+	The entry 6dba is ON HOLD until Paper Publication

	Authors: Brooks, C.L., Toride King, M., Huh, I.		Authors: Brooks, C.L., Toride King, M., Huh, I.

OCA: Protected "6dba" [edit=sysop:move=sysop]

2018-05-09T04:57:47Z

Protected "6dba" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 9 May 2018

OCA: New page: '''Unreleased structure''' The entry 6dba is ON HOLD Authors: Brooks, C.L., Toride King, M., Huh, I. Description: Crystal Structure of VHH R303 Category: Unreleased Structures [[Ca...

2018-05-09T04:57:46Z

New page: '''Unreleased structure''' The entry 6dba is ON HOLD Authors: Brooks, C.L., Toride King, M., Huh, I. Description: Crystal Structure of VHH R303 Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 6dba is ON HOLD

Authors: Brooks, C.L., Toride King, M., Huh, I.

Description: Crystal Structure of VHH R303
[[Category: Unreleased Structures]]
[[Category: Huh, I]]
[[Category: Brooks, C.L]]
[[Category: Toride King, M]]