6dq1 - Revision history

OCA at 06:12, 11 October 2023

2023-10-11T06:12:29Z

←Older revision		Revision as of 06:12, 11 October 2023
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	<StructureSection load='6dq1' size='340' side='right'caption='[[6dq1]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='6dq1' size='340' side='right'caption='[[6dq1]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dq1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQ1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6DQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DQ1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.598Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=~~PPN~~:~~PARA-NITROPHENYLALANINE~~'>~~PPN~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[6dq0\|6dq0]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dq1 OCA], [https://pdbe.org/6dq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dq1 RCSB], [https://www.ebi.ac.uk/pdbsum/6dq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dq1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dq1 OCA], [~~http~~://pdbe.org/6dq1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6dq1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6dq1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6dq1 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lee, B]]	+	[[Category: Lee B]]
-	[[Category: Phillips-Piro~~, C M~~]]	+	[[Category: Phillips-Piro CM]]
-	[[Category: Savidge, N]]	+	[[Category: Savidge N]]
-	~~[[Category: 4-nitro-l-phenylalanine]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Super folder gfp]]~~	+
-	~~[[Category: Unnatural amino acid~~]]	+

OCA at 08:33, 27 November 2019

2019-11-27T08:33:31Z

←Older revision		Revision as of 08:33, 27 November 2019
Line 1:		Line 1:

	==sfGFP N149 mutated to 4-nitro-L-phenylalanine==		==sfGFP N149 mutated to 4-nitro-L-phenylalanine==
-	<StructureSection load='6dq1' size='340' side='right' caption='[[6dq1]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='6dq1' size='340' side='right'caption='[[6dq1]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dq1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DQ1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 6dq1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6dq1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
		+	[[Category: Large Structures]]
	[[Category: Lee, B]]		[[Category: Lee, B]]
	[[Category: Phillips-Piro, C M]]		[[Category: Phillips-Piro, C M]]

OCA at 07:58, 17 October 2018

2018-10-17T07:58:36Z

←Older revision		Revision as of 07:58, 17 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6dq1 is ~~ON HOLD until Paper~~ Publication	+	==sfGFP N149 mutated to 4-nitro-L-phenylalanine==
		+	<StructureSection load='6dq1' size='340' side='right' caption='[[6dq1]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6dq1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DQ1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6dq0\|6dq0]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dq1 OCA], [http://pdbe.org/6dq1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/6dq1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dq1 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The X-ray crystal structures of two superfolder green fluorescent protein (sfGFP) constructs containing a genetically incorporated spectroscopic reporter unnatural amino acid, 4-nitro-L-phenylalanine (pNO2F), at two unique sites in the protein have been determined. Amber codon-suppression methodology was used to site-specifically incorporate pNO2F at a solvent-accessible (Asp133) and a partially buried (Asn149) site in sfGFP. The Asp133pNO2F sfGFP construct crystallized with two molecules per asymmetric unit in space group P3221 and the crystal structure was refined to 2.05 A resolution. Crystals of Asn149pNO2F sfGFP contained one molecule of sfGFP per asymmetric unit in space group P4122 and the structure was refined to 1.60 A resolution. The alignment of Asp133pNO2F or Asn149pNO2F sfGFP with wild-type sfGFP resulted in small root-mean-square deviations, illustrating that these residues do not significantly alter the protein structure and supporting the use of pNO2F as an effective spectroscopic reporter of local protein structure and dynamics.

-	~~Authors: Phillips~~-~~Piro, C.M~~., ~~Savidge~~, N., Lee, B.	+	Crystal structures of green fluorescent protein with the unnatural amino acid 4-nitro-L-phenylalanine.,Maurici N, Savidge N, Lee BU, Brewer SH, Phillips-Piro CM Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):650-655. doi:, 10.1107/S2053230X1801169X. Epub 2018 Sep 19. PMID:30279317<ref>PMID:30279317</ref>

-	~~Description: sfGFP N149 mutated to 4~~-~~nitro~~-~~L-phenylalanine~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Phillips-Piro, C.M]]~~	+	<div class="pdbe-citations 6dq1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lee, B]]		[[Category: Lee, B]]
		+	[[Category: Phillips-Piro, C M]]
	[[Category: Savidge, N]]		[[Category: Savidge, N]]
		+	[[Category: 4-nitro-l-phenylalanine]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Super folder gfp]]
		+	[[Category: Unnatural amino acid]]

OCA at 05:57, 12 September 2018

2018-09-12T05:57:40Z

←Older revision		Revision as of 05:57, 12 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dq1 is ON HOLD	+	The entry 6dq1 is ON HOLD until Paper Publication

	Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.		Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.

OCA at 14:44, 15 August 2018

2018-08-15T14:44:48Z

←Older revision		Revision as of 14:44, 15 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dq1 is ON HOLD ~~until Paper Publication~~	+	The entry 6dq1 is ON HOLD

	Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.		Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.

OCA at 05:39, 4 July 2018

2018-07-04T05:39:44Z

←Older revision		Revision as of 05:39, 4 July 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dq1 is ON HOLD	+	The entry 6dq1 is ON HOLD until Paper Publication

	Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.		Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.

OCA: Protected "6dq1" [edit=sysop:move=sysop]

2018-06-20T04:06:55Z

Protected "6dq1" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 20 June 2018

OCA: New page: '''Unreleased structure''' The entry 6dq1 is ON HOLD Authors: Phillips-Piro, C.M., Savidge, N., Lee, B. Description: sfGFP N149 mutated to 4-nitro-L-phenylalanine [[Category: Unrelease...

2018-06-20T04:06:54Z

New page: '''Unreleased structure''' The entry 6dq1 is ON HOLD Authors: Phillips-Piro, C.M., Savidge, N., Lee, B. Description: sfGFP N149 mutated to 4-nitro-L-phenylalanine [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 6dq1 is ON HOLD

Authors: Phillips-Piro, C.M., Savidge, N., Lee, B.

Description: sfGFP N149 mutated to 4-nitro-L-phenylalanine
[[Category: Unreleased Structures]]
[[Category: Phillips-Piro, C.M]]
[[Category: Lee, B]]
[[Category: Savidge, N]]