6dx9 - Revision history

OCA at 08:35, 27 November 2019

2019-11-27T08:35:37Z

←Older revision		Revision as of 08:35, 27 November 2019
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	==Crystal structure of chalcone synthase from Equisetum arvense==		==Crystal structure of chalcone synthase from Equisetum arvense==
-	<StructureSection load='6dx9' size='340' side='right' caption='[[6dx9]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='6dx9' size='340' side='right'caption='[[6dx9]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6dx9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_horsetail Common horsetail]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DX9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6dx9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_horsetail Common horsetail]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DX9 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Common horsetail]]		[[Category: Common horsetail]]
		+	[[Category: Large Structures]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Chiang, Y C]]		[[Category: Chiang, Y C]]

OCA at 08:51, 19 December 2018

2018-12-19T08:51:52Z

←Older revision		Revision as of 08:51, 19 December 2018
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	<StructureSection load='6dx9' size='340' side='right' caption='[[6dx9]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6dx9' size='340' side='right' caption='[[6dx9]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dx9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dx9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_horsetail Common horsetail]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DX9 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3258 Common horsetail])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dx9 OCA], [http://pdbe.org/6dx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dx9 RCSB], [http://www.ebi.ac.uk/pdbsum/6dx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dx9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dx9 OCA], [http://pdbe.org/6dx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dx9 RCSB], [http://www.ebi.ac.uk/pdbsum/6dx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dx9 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Common horsetail]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Chiang, Y C]]		[[Category: Chiang, Y C]]

OCA at 07:59, 17 October 2018

2018-10-17T07:59:12Z

←Older revision		Revision as of 07:59, 17 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6dx9 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of chalcone synthase from Equisetum arvense==
		+	<StructureSection load='6dx9' size='340' side='right' caption='[[6dx9]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6dx9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DX9 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dx9 OCA], [http://pdbe.org/6dx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dx9 RCSB], [http://www.ebi.ac.uk/pdbsum/6dx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dx9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHSY_EQUAR CHSY_EQUAR]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavonoids are important polyphenolic natural products, ubiquitous in land plants, that play diverse functions in plants' survival in their ecological niches, including UV protection, pigmentation for attracting pollinators, symbiotic nitrogen fixation, and defense against herbivores. Chalcone synthase (CHS) catalyzes the first committed step in plant flavonoid biosynthesis and is highly conserved in all land plants. In several previously reported crystal structures of CHSs from flowering plants, the catalytic cysteine is oxidized to sulfinic acid, indicating enhanced nucleophilicity in this residue associated with its increased susceptibility to oxidation. In this study, we report a set of new crystal structures of CHSs representing all five major lineages of land plants (bryophytes, lycophytes, monilophytes, gymnosperms, and angiosperms), spanning 500 million years of evolution. We reveal that the structures of CHS from a lycophyte and a moss species preserve the catalytic cysteine in a reduced state, in contrast to the cysteine sulfinic acid seen in all euphyllophyte CHS structures. In vivo complementation, in vitro biochemical and mutagenesis analyses, and molecular dynamics simulations identified a set of residues that differ between basal-plant and euphyllophyte CHSs and modulate catalytic cysteine reactivity. We propose that the CHS active-site environment has evolved in euphyllophytes to further enhance the nucleophilicity of the catalytic cysteine since the divergence of euphyllophytes from other vascular plant lineages 400 million years ago. These changes in CHS could have contributed to the diversification of flavonoid biosynthesis in euphyllophytes, which in turn contributed to their dominance in terrestrial ecosystems.

-	~~Authors: Weng, J.K~~., Liou, G., Chiang, Y.C.~~, Wang, Y~~.	+	Mechanistic basis for the evolution of chalcone synthase catalytic cysteine reactivity in land plants.,Liou G, Chiang YC, Wang Y, Weng JK J Biol Chem. 2018 Oct 5. pii: RA118.005695. doi: 10.1074/jbc.RA118.005695. PMID:30291143<ref>PMID:30291143</ref>

-	~~Description: Crystal structure~~ of chalcone synthase ~~from Equisetum arvense~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6dx9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Naringenin-chalcone synthase]]
		+	[[Category: Chiang, Y C]]
	[[Category: Liou, G]]		[[Category: Liou, G]]
-	[[Category: Weng, J.K]]
-	[[Category: Chiang, Y.C]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
		+	[[Category: Weng, J K]]
		+	[[Category: Flavonoid]]
		+	[[Category: Polyketide synthase]]
		+	[[Category: Thiolase]]
		+	[[Category: Transferase]]

OCA at 17:00, 1 August 2018

2018-08-01T17:00:02Z

←Older revision		Revision as of 17:00, 1 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dx9 is ON HOLD	+	The entry 6dx9 is ON HOLD until Paper Publication

	Authors: Weng, J.K., Liou, G., Chiang, Y.C., Wang, Y.		Authors: Weng, J.K., Liou, G., Chiang, Y.C., Wang, Y.

OCA: Protected "6dx9" [edit=sysop:move=sysop]

2018-07-04T05:49:58Z

Protected "6dx9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 4 July 2018

OCA: New page: '''Unreleased structure''' The entry 6dx9 is ON HOLD Authors: Weng, J.K., Liou, G., Chiang, Y.C., Wang, Y. Description: Crystal structure of chalcone synthase from Equisetum arvense [[...

2018-07-04T05:49:57Z

New page: '''Unreleased structure''' The entry 6dx9 is ON HOLD Authors: Weng, J.K., Liou, G., Chiang, Y.C., Wang, Y. Description: Crystal structure of chalcone synthase from Equisetum arvense [[...

New page

'''Unreleased structure'''

The entry 6dx9 is ON HOLD

Authors: Weng, J.K., Liou, G., Chiang, Y.C., Wang, Y.

Description: Crystal structure of chalcone synthase from Equisetum arvense
[[Category: Unreleased Structures]]
[[Category: Liou, G]]
[[Category: Weng, J.K]]
[[Category: Chiang, Y.C]]
[[Category: Wang, Y]]