6dxd - Revision history

OCA at 08:36, 27 November 2019

2019-11-27T08:36:00Z

←Older revision		Revision as of 08:36, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of chalcone synthase from Arabidopsis thaliana - C347S mutant==		==Crystal structure of chalcone synthase from Arabidopsis thaliana - C347S mutant==
-	<StructureSection load='6dxd' size='340' side='right' caption='[[6dxd]], [[Resolution\|resolution]] 1.59Å' scene=''>	+	<StructureSection load='6dxd' size='340' side='right'caption='[[6dxd]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6dxd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DXD FirstGlance]. <br>		<table><tr><td colspan='2'>[[6dxd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DXD FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Chiang, Y C]]		[[Category: Chiang, Y C]]

OCA at 08:52, 19 December 2018

2018-12-19T08:52:12Z

←Older revision		Revision as of 08:52, 19 December 2018
Line 3:		Line 3:
	<StructureSection load='6dxd' size='340' side='right' caption='[[6dxd]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='6dxd' size='340' side='right' caption='[[6dxd]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dxd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DXD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dxd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DXD FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHS, TT4, At5g13930, MAC12.11, MAC12.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dxd OCA], [http://pdbe.org/6dxd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dxd RCSB], [http://www.ebi.ac.uk/pdbsum/6dxd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dxd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dxd OCA], [http://pdbe.org/6dxd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dxd RCSB], [http://www.ebi.ac.uk/pdbsum/6dxd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dxd ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Chiang, Y C]]		[[Category: Chiang, Y C]]

OCA at 07:59, 17 October 2018

2018-10-17T07:59:32Z

←Older revision		Revision as of 07:59, 17 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6dxd is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of chalcone synthase from Arabidopsis thaliana - C347S mutant==
		+	<StructureSection load='6dxd' size='340' side='right' caption='[[6dxd]], [[Resolution\|resolution]] 1.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6dxd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DXD FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dxd OCA], [http://pdbe.org/6dxd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dxd RCSB], [http://www.ebi.ac.uk/pdbsum/6dxd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dxd ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHSY_ARATH CHSY_ARATH]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavonoids are important polyphenolic natural products, ubiquitous in land plants, that play diverse functions in plants' survival in their ecological niches, including UV protection, pigmentation for attracting pollinators, symbiotic nitrogen fixation, and defense against herbivores. Chalcone synthase (CHS) catalyzes the first committed step in plant flavonoid biosynthesis and is highly conserved in all land plants. In several previously reported crystal structures of CHSs from flowering plants, the catalytic cysteine is oxidized to sulfinic acid, indicating enhanced nucleophilicity in this residue associated with its increased susceptibility to oxidation. In this study, we report a set of new crystal structures of CHSs representing all five major lineages of land plants (bryophytes, lycophytes, monilophytes, gymnosperms, and angiosperms), spanning 500 million years of evolution. We reveal that the structures of CHS from a lycophyte and a moss species preserve the catalytic cysteine in a reduced state, in contrast to the cysteine sulfinic acid seen in all euphyllophyte CHS structures. In vivo complementation, in vitro biochemical and mutagenesis analyses, and molecular dynamics simulations identified a set of residues that differ between basal-plant and euphyllophyte CHSs and modulate catalytic cysteine reactivity. We propose that the CHS active-site environment has evolved in euphyllophytes to further enhance the nucleophilicity of the catalytic cysteine since the divergence of euphyllophytes from other vascular plant lineages 400 million years ago. These changes in CHS could have contributed to the diversification of flavonoid biosynthesis in euphyllophytes, which in turn contributed to their dominance in terrestrial ecosystems.

-	~~Authors: Liou~~, G., Chiang~~, Y.C.~~, Wang, Y., Weng, J.K.	+	Mechanistic basis for the evolution of chalcone synthase catalytic cysteine reactivity in land plants.,Liou G, Chiang YC, Wang Y, Weng JK J Biol Chem. 2018 Oct 5. pii: RA118.005695. doi: 10.1074/jbc.RA118.005695. PMID:30291143<ref>PMID:30291143</ref>

-	~~Description: Crystal structure~~ of chalcone synthase ~~from Arabidopsis thaliana -C347S mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6dxd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Naringenin-chalcone synthase]]
		+	[[Category: Chiang, Y C]]
	[[Category: Liou, G]]		[[Category: Liou, G]]
-	[[Category: Weng, J.K]]
-	[[Category: Chiang, Y.C]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
		+	[[Category: Weng, J K]]
		+	[[Category: Flavonoid]]
		+	[[Category: Polyketide synthase]]
		+	[[Category: Thiolase]]
		+	[[Category: Transferase]]

OCA at 17:00, 1 August 2018

2018-08-01T17:00:15Z

←Older revision		Revision as of 17:00, 1 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dxd is ON HOLD	+	The entry 6dxd is ON HOLD until Paper Publication

	Authors: Liou, G., Chiang, Y.C., Wang, Y., Weng, J.K.		Authors: Liou, G., Chiang, Y.C., Wang, Y., Weng, J.K.

OCA: Protected "6dxd" [edit=sysop:move=sysop]

2018-07-04T05:50:10Z

Protected "6dxd" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 4 July 2018

OCA: New page: '''Unreleased structure''' The entry 6dxd is ON HOLD Authors: Liou, G., Chiang, Y.C., Wang, Y., Weng, J.K. Description: Crystal structure of chalcone synthase from Arabidopsis thaliana...

2018-07-04T05:50:08Z

New page: '''Unreleased structure''' The entry 6dxd is ON HOLD Authors: Liou, G., Chiang, Y.C., Wang, Y., Weng, J.K. Description: Crystal structure of chalcone synthase from Arabidopsis thaliana...

New page

'''Unreleased structure'''

The entry 6dxd is ON HOLD

Authors: Liou, G., Chiang, Y.C., Wang, Y., Weng, J.K.

Description: Crystal structure of chalcone synthase from Arabidopsis thaliana -C347S mutant
[[Category: Unreleased Structures]]
[[Category: Liou, G]]
[[Category: Weng, J.K]]
[[Category: Chiang, Y.C]]
[[Category: Wang, Y]]