6e0d - Revision history

OCA at 09:08, 11 November 2020

2020-11-11T09:08:58Z

←Older revision		Revision as of 09:08, 11 November 2020
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	==X-ray structure of a complex of thaumatin with xylene cyanol==		==X-ray structure of a complex of thaumatin with xylene cyanol==
-	<StructureSection load='6e0d' size='340' side='right' caption='[[6e0d]], [[Resolution\|resolution]] 2.24Å' scene=''>	+	<StructureSection load='6e0d' size='340' side='right'caption='[[6e0d]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e0d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0D OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6E0D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e0d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6E0D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>, <scene name='pdbligand=XXX:(2R,3S,4R,5R,6R)-6-((1R,2R,3S,4R,6S)-4,6-DIAMINO-2,3-DIHYDROXYCYCLOHEXYLOXY)-5-AMINO-2-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-3,4-DIOL'>XXX</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>, <scene name='pdbligand=XXX:(2R,3S,4R,5R,6R)-6-((1R,2R,3S,4R,6S)-4,6-DIAMINO-2,3-DIHYDROXYCYCLOHEXYLOXY)-5-AMINO-2-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-3,4-DIOL'>XXX</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6e0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0d OCA], [http://pdbe.org/6e0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0d RCSB], [http://www.ebi.ac.uk/pdbsum/6e0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6e0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0d OCA], [http://pdbe.org/6e0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0d RCSB], [http://www.ebi.ac.uk/pdbsum/6e0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thaumatococcus daniellii]]		[[Category: Thaumatococcus daniellii]]
	[[Category: McPherson, A]]		[[Category: McPherson, A]]

OCA at 19:51, 19 September 2018

2018-09-19T19:51:23Z

←Older revision		Revision as of 19:51, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6e0d is ~~ON HOLD until Paper~~ Publication	+	==X-ray structure of a complex of thaumatin with xylene cyanol==
		+	<StructureSection load='6e0d' size='340' side='right' caption='[[6e0d]], [[Resolution\|resolution]] 2.24Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6e0d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0D FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>, <scene name='pdbligand=XXX:(2R,3S,4R,5R,6R)-6-((1R,2R,3S,4R,6S)-4,6-DIAMINO-2,3-DIHYDROXYCYCLOHEXYLOXY)-5-AMINO-2-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-3,4-DIOL'>XXX</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0d OCA], [http://pdbe.org/6e0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0d RCSB], [http://www.ebi.ac.uk/pdbsum/6e0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0d ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THM1_THADA THM1_THADA]] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It was found that the crystals of at least a dozen different proteins could be thoroughly stained to an intense color with a panel of dyes. Many, if not most, of the stained protein crystals retained the dyes almost indefinitely when placed in large volumes of dye-free mother liquor. Dialysis experiments showed that most of the dyes that were retained in crystals also bound to the protein when free in solution; less frequently, some dyes bound only in the crystal. The experiments indicated a strong association of the dyes with the proteins. Four protein crystals were investigated by X-ray diffraction to ascertain the mode of binding. These were crystals of lysozyme, thaumatin, trypsin inhibited with benzamidine and satellite tobacco mosaic virus. In 30 X-ray analyses of protein crystal-dye complexes, in only three difference Fourier maps was any difference electron density present that was consistent with the binding of dye molecules, and even in these three cases (thaumatin plus thioflavin T, xylene cyanol and m-cresol purple) the amount of dye observed was inadequate to explain the intense color of the crystals. It was concluded that the dye molecules, which are clearly inside the crystals, are disordered but are paradoxically tightly bound to the protein. It is speculated that the dyes, which exhibit large hydrophobic cores and peripheral charged groups, may interact with the crystalline proteins in the manner of conventional detergents.

-	~~Authors: McPherson~~, A.	+	Investigation into the binding of dyes within protein crystals.,McPherson A, Larson SB Acta Crystallogr F Struct Biol Commun. 2018 Sep 1;74(Pt 9):593-602. doi:, 10.1107/S2053230X18010300. Epub 2018 Sep 3. PMID:30198893<ref>PMID:30198893</ref>

-	~~Description: X-ray structure of~~ a ~~complex~~ of ~~thaumatin with xylene cyanol~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Mcpherson~~, A]]	+	<div class="pdbe-citations 6e0d" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Thaumatococcus daniellii]]
		+	[[Category: McPherson, A]]
		+	[[Category: Disulfide]]
		+	[[Category: Dye binding]]
		+	[[Category: Plant protein]]
		+	[[Category: Plant protein-antibiotic complex]]
		+	[[Category: Sweet tasting protein]]
		+	[[Category: Xylene cyanol]]

OCA at 17:00, 1 August 2018

2018-08-01T17:00:58Z

←Older revision		Revision as of 17:00, 1 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6e0d is ON HOLD	+	The entry 6e0d is ON HOLD until Paper Publication

	Authors: McPherson, A.		Authors: McPherson, A.

OCA: Protected "6e0d" [edit=sysop:move=sysop]

2018-07-18T05:30:51Z

Protected "6e0d" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6e0d is ON HOLD Authors: McPherson, A. Description: X-ray structure of a complex of thaumatin with xylene cyanol [[Category: Unreleased Structures]...

2018-07-18T05:30:48Z

New page: '''Unreleased structure''' The entry 6e0d is ON HOLD Authors: McPherson, A. Description: X-ray structure of a complex of thaumatin with xylene cyanol [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 6e0d is ON HOLD

Authors: McPherson, A.

Description: X-ray structure of a complex of thaumatin with xylene cyanol
[[Category: Unreleased Structures]]
[[Category: Mcpherson, A]]