6e7e - Revision history

OCA at 14:39, 13 March 2024

2024-03-13T14:39:49Z

←Older revision		Revision as of 14:39, 13 March 2024
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	<StructureSection load='6e7e' size='340' side='right'caption='[[6e7e]], [[Resolution\|resolution]] 1.12Å' scene=''>		<StructureSection load='6e7e' size='340' side='right'caption='[[6e7e]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e7e]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"chlamydozoon_trachomatis"_(busacca_1935)_moshkovski_1945 "chlamydozoon~~ trachomatis~~" (busacca 1935) moshkovski 1945~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E7E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6E7E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e7e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E7E FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>incA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~813~~ "~~Chlamydozoon trachomatis~~" ~~(Busacca 1935) Moshkovski 1945~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.12Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6e7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e7e OCA], [~~http~~://pdbe.org/6e7e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6e7e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6e7e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6e7e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e7e OCA], [https://pdbe.org/6e7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e7e RCSB], [https://www.ebi.ac.uk/pdbsum/6e7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e7e ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style="background-color:#fffaf0;">~~	+	== Function ==
-	== ~~Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/INCA_CHLTR INCA_CHLTR] Chlamydia replicate within an intracellular vacuole, termed an inclusion. IncA is probably involved in the homotypic fusion of inclusions.[UniProtKB:A0A0H3MD02]
-	~~Many intracellular bacteria, including Chlamydia, establish a parasitic membrane-bound organelle inside the host cell that is essential for the bacteria's survival~~. Chlamydia ~~trachomatis forms inclusions that are decorated with poorly characterized membrane proteins known as Incs. The prototypical Inc~~, ~~called IncA, enhances Chlamydia pathogenicity by promoting the homotypic fusion of inclusions and shares structural and functional similarity to eukaryotic SNAREs~~. ~~Here, we present the atomic structure of the cytoplasmic domain of~~ IncA~~, which reveals a non-canonical four-helix bundle. Structure-based mutagenesis, molecular dynamics simulation, and functional cellular assays identify an intramolecular clamp that~~ is ~~essential for IncA-mediated homotypic membrane fusion during infection.~~	+
-		+
-	~~Structural basis for~~ the homotypic fusion of ~~chlamydial~~ inclusions ~~by the SNARE-like protein IncA~~.~~,Cingolani G, McCauley M, Lobley A, Bryer AJ, Wesolowski J, Greco DL, Lokareddy RK, Ronzone E, Perilla JR, Paumet F Nat Commun. 2019 Jun 21;10(1)~~:~~2747. doi: 10.1038/s41467-019-10806-9. PMID:31227715<ref>PMID:31227715</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6e7e" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlamydia trachomatis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cingolani, G]]	+	[[Category: Cingolani G]]
-	[[Category: Paumet, F]]	+	[[Category: Paumet F]]
-	~~[[Category: Chlamydia]]~~	+
-	~~[[Category: Inclusion]]~~	+
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Snare-like protein~~]]	+

OCA at 10:12, 4 December 2019

2019-12-04T10:12:43Z

←Older revision		Revision as of 10:12, 4 December 2019
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	<StructureSection load='6e7e' size='340' side='right'caption='[[6e7e]], [[Resolution\|resolution]] 1.12Å' scene=''>		<StructureSection load='6e7e' size='340' side='right'caption='[[6e7e]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e7e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E7E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e7e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"chlamydozoon_trachomatis"_(busacca_1935)_moshkovski_1945 "chlamydozoon trachomatis" (busacca 1935) moshkovski 1945]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E7E FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e7e OCA], [http://pdbe.org/6e7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e7e RCSB], [http://www.ebi.ac.uk/pdbsum/6e7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e7e ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">incA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 "Chlamydozoon trachomatis" (Busacca 1935) Moshkovski 1945])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e7e OCA], [http://pdbe.org/6e7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e7e RCSB], [http://www.ebi.ac.uk/pdbsum/6e7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e7e ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:41, 10 July 2019

2019-07-10T05:41:32Z

←Older revision		Revision as of 05:41, 10 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6e7e is ~~ON HOLD~~	+	==High resolution crystal structure of IncA soluble domain==
		+	<StructureSection load='6e7e' size='340' side='right'caption='[[6e7e]], [[Resolution\|resolution]] 1.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6e7e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E7E FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e7e OCA], [http://pdbe.org/6e7e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e7e RCSB], [http://www.ebi.ac.uk/pdbsum/6e7e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e7e ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many intracellular bacteria, including Chlamydia, establish a parasitic membrane-bound organelle inside the host cell that is essential for the bacteria's survival. Chlamydia trachomatis forms inclusions that are decorated with poorly characterized membrane proteins known as Incs. The prototypical Inc, called IncA, enhances Chlamydia pathogenicity by promoting the homotypic fusion of inclusions and shares structural and functional similarity to eukaryotic SNAREs. Here, we present the atomic structure of the cytoplasmic domain of IncA, which reveals a non-canonical four-helix bundle. Structure-based mutagenesis, molecular dynamics simulation, and functional cellular assays identify an intramolecular clamp that is essential for IncA-mediated homotypic membrane fusion during infection.

-	~~Authors: Cingolani~~, G., ~~Paumet~~, F.	+	Structural basis for the homotypic fusion of chlamydial inclusions by the SNARE-like protein IncA.,Cingolani G, McCauley M, Lobley A, Bryer AJ, Wesolowski J, Greco DL, Lokareddy RK, Ronzone E, Perilla JR, Paumet F Nat Commun. 2019 Jun 21;10(1):2747. doi: 10.1038/s41467-019-10806-9. PMID:31227715<ref>PMID:31227715</ref>

-	~~Description: High resolution crystal structure~~ of ~~IncA soluble domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6e7e" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Paumet, F]]		[[Category: Paumet, F]]
		+	[[Category: Chlamydia]]
		+	[[Category: Inclusion]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Protein binding]]
		+	[[Category: Snare-like protein]]

OCA at 04:00, 19 June 2019

2019-06-19T04:00:39Z

←Older revision		Revision as of 04:00, 19 June 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6e7e is ON HOLD ~~until Jul 26 2020~~	+	The entry 6e7e is ON HOLD

	Authors: Cingolani, G., Paumet, F.		Authors: Cingolani, G., Paumet, F.

OCA at 04:39, 22 August 2018

2018-08-22T04:39:50Z

←Older revision		Revision as of 04:39, 22 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6e7e is ON HOLD	+	The entry 6e7e is ON HOLD until Jul 26 2020

	Authors: Cingolani, G., Paumet, F.		Authors: Cingolani, G., Paumet, F.

OCA: Protected "6e7e" [edit=sysop:move=sysop]

2018-08-01T17:09:09Z

Protected "6e7e" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:09, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6e7e is ON HOLD Authors: Cingolani, G., Paumet, F. Description: High resolution crystal structure of IncA soluble domain [[Category: Unreleased Str...

2018-08-01T17:09:07Z

New page: '''Unreleased structure''' The entry 6e7e is ON HOLD Authors: Cingolani, G., Paumet, F. Description: High resolution crystal structure of IncA soluble domain [[Category: Unreleased Str...

New page

'''Unreleased structure'''

The entry 6e7e is ON HOLD

Authors: Cingolani, G., Paumet, F.

Description: High resolution crystal structure of IncA soluble domain
[[Category: Unreleased Structures]]
[[Category: Cingolani, G]]
[[Category: Paumet, F]]