6eb0 - Revision history

OCA at 06:22, 11 October 2023

2023-10-11T06:22:12Z

←Older revision		Revision as of 06:22, 11 October 2023
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	<StructureSection load='6eb0' size='340' side='right'caption='[[6eb0]], [[Resolution\|resolution]] 2.37Å' scene=''>		<StructureSection load='6eb0' size='340' side='right'caption='[[6eb0]], [[Resolution\|resolution]] 2.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eb0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EB0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EB0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eb0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EB0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand~~=~~ACT:ACETATE+ION'>ACT</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.37Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6b1b\|6b1b]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eb0 OCA], [~~http~~://pdbe.org/6eb0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6eb0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6eb0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6eb0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eb0 OCA], [https://pdbe.org/6eb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eb0 RCSB], [https://www.ebi.ac.uk/pdbsum/6eb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eb0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A140NG21_ECOBD A0A140NG21_ECOBD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6eb0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6eb0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kandavelu, P]]	+	[[Category: Kandavelu P]]
-	[[Category: Rose, J]]	+	[[Category: Rose J]]
-	[[Category: Wang~~, B C~~]]	+	[[Category: Wang BC]]
-	[[Category: Yan, Y]]	+	[[Category: Yan Y]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	[[Category: Zhou, D]]	+	[[Category: Zhou D]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxygenase component]]~~	+
-	~~[[Category: Protein engineering: 4-hydroxyphenylacetate 3-monooxygenase~~]]	+

OCA at 06:44, 23 May 2019

2019-05-23T06:44:51Z

←Older revision		Revision as of 06:44, 23 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6eb0 is ~~ON HOLD until Paper~~ Publication	+	==STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI==
		+	<StructureSection load='6eb0' size='340' side='right'caption='[[6eb0]], [[Resolution\|resolution]] 2.37Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6eb0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EB0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b1b\|6b1b]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eb0 OCA], [http://pdbe.org/6eb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eb0 RCSB], [http://www.ebi.ac.uk/pdbsum/6eb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eb0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	4-Hydroxyphenylacetate 3-hydroxylase (EcHpaB) from Escherichia coli is capable of efficient ortho-hydroxylation of a wide range of phenolic compounds and demonstrates great potential for broad chemoenzymatic applications. To understand the structural and mechanistic basis of its catalytic versatility, we elucidated the crystal structure of EcHpaB by X-ray crystallography, which revealed a unique loop structure covering the active site. We further performed mutagenesis studies of this loop to probe its role in substrate specificity and catalytic activity. Our results not only showed the loop has great plasticity and strong tolerance towards extensive mutagenesis, but also suggested a flexible loop that enables the entrance and stable binding of substrates into the active site is the key factor to the enzyme catalytic versatility. These findings lay the groundwork for editing the loop sequence and structure for generation of EcHpaB mutants with improved performance for broader laboratory and industrial use.

-	~~Authors~~:	+	Structural Insights into Catalytic Versatility of the Flavin-dependent Hydroxylase (HpaB) from Escherichia coli.,Shen X, Zhou D, Lin Y, Wang J, Gao S, Kandavelu P, Zhang H, Zhang R, Wang BC, Rose J, Yuan Q, Yan Y Sci Rep. 2019 May 8;9(1):7087. doi: 10.1038/s41598-019-43577-w. PMID:31068633<ref>PMID:31068633</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6eb0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kandavelu, P]]
		+	[[Category: Rose, J]]
		+	[[Category: Wang, B C]]
		+	[[Category: Yan, Y]]
		+	[[Category: Zhang, H]]
		+	[[Category: Zhou, D]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxygenase component]]
		+	[[Category: Protein engineering: 4-hydroxyphenylacetate 3-monooxygenase]]

OCA at 05:42, 29 August 2018

2018-08-29T05:42:57Z

←Older revision		Revision as of 05:42, 29 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6eb0 is ON HOLD	+	The entry 6eb0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6eb0" [edit=sysop:move=sysop]

2018-08-15T14:48:26Z

Protected "6eb0" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:48, 15 August 2018

OCA: New page: '''Unreleased structure''' The entry 6eb0 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-08-15T14:48:24Z

New page: '''Unreleased structure''' The entry 6eb0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6eb0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]