6ee5 - Revision history

OCA at 06:24, 11 October 2023

2023-10-11T06:24:29Z

←Older revision		Revision as of 06:24, 11 October 2023
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	<StructureSection load='6ee5' size='340' side='right'caption='[[6ee5]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='6ee5' size='340' side='right'caption='[[6ee5]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ee5]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ee5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EE5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee5 OCA], [~~http~~://pdbe.org/6ee5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ee5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ee5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.48Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee5 OCA], [https://pdbe.org/6ee5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ee5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ee5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Buckle~~, A M~~]]	+	[[Category: Buckle AM]]
-	[[Category: Marijanovic~~, E M~~]]	+	[[Category: Marijanovic EM]]
-	[[Category: McGowan, S]]	+	[[Category: McGowan S]]
-	[[Category: Porebski~~, B T]]~~	+	[[Category: Porebski BT]]
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Protein dynamic]]~~	+
-	~~[[Category: Protein engineering~~]]	+

OCA at 08:22, 20 March 2019

2019-03-20T08:22:18Z

←Older revision		Revision as of 08:22, 20 March 2019
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	==Reactive centre loop dynamics and serpin specificity==		==Reactive centre loop dynamics and serpin specificity==
-	<StructureSection load='6ee5' size='340' side='right' caption='[[6ee5]], [[Resolution\|resolution]] 2.48Å' scene=''>	+	<StructureSection load='6ee5' size='340' side='right'caption='[[6ee5]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ee5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ee5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EE5 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee5 OCA], [http://pdbe.org/6ee5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ee5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ee5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee5 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee5 OCA], [http://pdbe.org/6ee5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ee5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ee5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee5 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serine proteinase inhibitors (serpins), typically fold to a metastable native state and undergo a major conformational change in order to inhibit target proteases. However, conformational lability of the native serpin fold renders them susceptible to misfolding and aggregation, and underlies misfolding diseases such as alpha1-antitrypsin deficiency. Serpin specificity towards its protease target is dictated by its flexible and solvent exposed reactive centre loop (RCL), which forms the initial interaction with the target protease during inhibition. Previous studies have attempted to alter the specificity by mutating the RCL to that of a target serpin, but the rules governing specificity are not understood well enough yet to enable specificity to be engineered at will. In this paper, we use conserpin, a synthetic, thermostable serpin, as a model protein with which to investigate the determinants of serpin specificity by engineering its RCL. Replacing the RCL sequence with that from alpha1-antitrypsin fails to restore specificity against trypsin or human neutrophil elastase. Structural determination of the RCL-engineered conserpin and molecular dynamics simulations indicate that, although the RCL sequence may partially dictate specificity, local electrostatics and RCL dynamics may dictate the rate of insertion during protease inhibition, and thus whether it behaves as an inhibitor or a substrate. Engineering serpin specificity is therefore substantially more complex than solely manipulating the RCL sequence, and will require a more thorough understanding of how conformational dynamics achieves the delicate balance between stability, folding and function required by the exquisite serpin mechanism of action.
		+
		+	Reactive centre loop dynamics and serpin specificity.,Marijanovic EM, Fodor J, Riley BT, Porebski BT, Costa MGS, Kass I, Hoke DE, McGowan S, Buckle AM Sci Rep. 2019 Mar 7;9(1):3870. doi: 10.1038/s41598-019-40432-w. PMID:30846766<ref>PMID:30846766</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ee5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Buckle, A M]]		[[Category: Buckle, A M]]
	[[Category: Marijanovic, E M]]		[[Category: Marijanovic, E M]]

OCA at 07:29, 29 August 2018

2018-08-29T07:29:35Z

←Older revision		Revision as of 07:29, 29 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ee5 ~~is ON HOLD~~	+	==Reactive centre loop dynamics and serpin specificity==
-		+	<StructureSection load='6ee5' size='340' side='right' caption='[[6ee5]], [[Resolution\|resolution]] 2.48Å' scene=''>
-	~~Authors~~: ~~Marijanovic~~, E.M., ~~Porebski~~, B.T., ~~McGowan, S~~., ~~Buckle, A~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ee5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EE5 FirstGlance]. <br>
-	~~Description: Reactive centre loop dynamics and serpin specificity~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ee5 OCA], [http://pdbe.org/6ee5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ee5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ee5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ee5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Buckle~~, A.M]]	+	__TOC__
-	[[Category: ~~Marijanovic~~, ~~E.M~~]]	+	</StructureSection>
-	[[Category: Porebski, B.T]]	+	[[Category: Buckle, A M]]
-	[[Category: ~~Mcgowan, S~~]]	+	[[Category: Marijanovic, E M]]
		+	[[Category: McGowan, S]]
		+	[[Category: Porebski, B T]]
		+	[[Category: De novo protein]]
		+	[[Category: Protein dynamic]]
		+	[[Category: Protein engineering]]

OCA: Protected "6ee5" [edit=sysop:move=sysop]

2018-08-22T04:44:20Z

Protected "6ee5" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:44, 22 August 2018

OCA: New page: '''Unreleased structure''' The entry 6ee5 is ON HOLD Authors: Marijanovic, E.M., Porebski, B.T., McGowan, S., Buckle, A.M. Description: Reactive centre loop dynamics and serpin specifi...

2018-08-22T04:44:19Z

New page: '''Unreleased structure''' The entry 6ee5 is ON HOLD Authors: Marijanovic, E.M., Porebski, B.T., McGowan, S., Buckle, A.M. Description: Reactive centre loop dynamics and serpin specifi...

New page

'''Unreleased structure'''

The entry 6ee5 is ON HOLD

Authors: Marijanovic, E.M., Porebski, B.T., McGowan, S., Buckle, A.M.

Description: Reactive centre loop dynamics and serpin specificity
[[Category: Unreleased Structures]]
[[Category: Buckle, A.M]]
[[Category: Marijanovic, E.M]]
[[Category: Porebski, B.T]]
[[Category: Mcgowan, S]]